Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress
Abstract
:1. Introduction
2. Results
2.1. Expression Profiles of StCDPKs in Potato Plants under Water Deficiency and Osmotic Stress, and Analysis of CDPK28 Protein
2.2. Subcellular Localization of the StCDPK28 Protein and Construction of Potato Plants with Over- or Under-Expression of StCDPK28
2.3. StCDPK28 Was Responsive to Water Deficiency and Osmotic Stress in Photosynthesis
2.4. StCDPK28 Modulated the Contents of H2O2, Malondialdehyde (MDA), and Proline for Tolerance to Water Deficiency and Osmotic Stress
2.5. Catalase (CAT), Superoxide Dismutase (SOD) and Peroxidase (POD) Activities in StCDPK28-Overexpressing and StCDPK28-KnockDown Plants under Water Deficiency and Osmotic Stress
2.6. Sequence Accession Numbers
3. Discussion
4. Materials and Methods
4.1. Plasmid Construction and Transformation
4.2. Water Deficiency and Osmotic Stress Treatment
4.3. Validation of the Transgenic Potato Plants by PCR
4.4. Quantification of Gene Expression by qRT-PCR
4.5. Subcellular Location of StCDPK28
4.6. Photosynthesis, Transpiration, Stomatal Conductance and Water Use Efficiency
4.7. CAT, SOD and POD Activity
4.8. H2O2, MDA and Proline Contents
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhu, X.; Wang, F.; Li, S.; Feng, Y.; Yang, J.; Zhang, N.; Si, H. Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress. Int. J. Mol. Sci. 2022, 23, 8795. https://doi.org/10.3390/ijms23158795
Zhu X, Wang F, Li S, Feng Y, Yang J, Zhang N, Si H. Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress. International Journal of Molecular Sciences. 2022; 23(15):8795. https://doi.org/10.3390/ijms23158795
Chicago/Turabian StyleZhu, Xi, Fangfang Wang, Shigui Li, Ya Feng, Jiangwei Yang, Ning Zhang, and Huaijun Si. 2022. "Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress" International Journal of Molecular Sciences 23, no. 15: 8795. https://doi.org/10.3390/ijms23158795