Identification and Characterization of the Core Region of ZmDi19-5 Promoter Activity and Its Upstream Regulatory Proteins
Abstract
:1. Introduction
2. Results
2.1. Identification of ZmDi19 Genes
2.2. Phylogenetic Relationships, Gene Structure, Chromosomal Location, and Gene Duplication
2.3. Expression Pattern Analysis of ZmDi19 Genes at Different Developmental Stages
2.4. Sequence Analysis and Expressed Activity of ZmDi19-5 Promoter under Drought Stress
2.5. Identification of Core Region of ZmDi19-5 Promoter
2.6. Identification of Upstream Regulatory Proteins of ZmDi19-5
3. Discussion
4. Materials and Methods
4.1. Identification and Sequence Analysis of Di19 Genes in Maize
4.2. Phylogenetic Analysis
4.3. Expression Pattern Analysis Using Transcriptome Data
4.4. Cloning and Sequence Analysis of ZmDi19-5 Promoter
4.5. Transient Transformation in Tobacco
4.6. Determination of LUC Activity and Identification of Promoter Core Region
4.7. Yeast One-Hybrid Library Screening
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, Y.; Xu, L.; Huang, Y.; Wu, H.; Zhang, X.; Hu, X.; Ma, Q. Identification and Characterization of the Core Region of ZmDi19-5 Promoter Activity and Its Upstream Regulatory Proteins. Int. J. Mol. Sci. 2022, 23, 7390. https://doi.org/10.3390/ijms23137390
Zhao Y, Xu L, Huang Y, Wu H, Zhang X, Hu X, Ma Q. Identification and Characterization of the Core Region of ZmDi19-5 Promoter Activity and Its Upstream Regulatory Proteins. International Journal of Molecular Sciences. 2022; 23(13):7390. https://doi.org/10.3390/ijms23137390
Chicago/Turabian StyleZhao, Yang, Lijuan Xu, Yuanxiang Huang, Hongying Wu, Xingen Zhang, Xiaolin Hu, and Qing Ma. 2022. "Identification and Characterization of the Core Region of ZmDi19-5 Promoter Activity and Its Upstream Regulatory Proteins" International Journal of Molecular Sciences 23, no. 13: 7390. https://doi.org/10.3390/ijms23137390