Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process

Nishi, Ryota; Oda, Yudai; Morikura, Takashi; Miyata, Shogo

doi:10.3390/ijms23137091

Open AccessArticle

Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process

¹

Graduate School of Science and Technology, Keio University, Yokohama 223-8522, Japan

²

Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2022, 23(13), 7091; https://doi.org/10.3390/ijms23137091

Submission received: 12 June 2022 / Accepted: 23 June 2022 / Published: 25 June 2022

(This article belongs to the Special Issue Cancer Cell Metabolism 2.0)

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Abstract

In this study, we proposed an in vitro tumor model to simulate the mechanical microenvironment and investigate the effect of compressive stress on the invasion process of malignant tumors. It has been pointed out that the biomechanical environment, as well as the biochemical environment, could affect the transformation of cancer cell migration, invasion, and metastasis. We hypothesized that the solid stress caused by the exclusion of surrounding tissue could transform tumor cells from noninvasive to invasive phenotypes. Colorectal cell spheroids were embedded and cultured in agarose gels of varying concentrations to simulate the earliest stages of tumor formation and invasion. The spheroids embedded in gels at higher concentrations showed peculiar growth after 72 h of culture, and the external compressive loading imposed on them caused peculiar growth even in the gels at lower concentrations. In conclusion, the mechanical microenvironment caused the transformation of tumor cell phenotypes, promoting the growth and invasion of tumor cell spheroids.

Keywords: mechanobiology; tumor; spheroid; colorectal cancer; invasion

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MDPI and ACS Style

Nishi, R.; Oda, Y.; Morikura, T.; Miyata, S. Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process. Int. J. Mol. Sci. 2022, 23, 7091. https://doi.org/10.3390/ijms23137091

AMA Style

Nishi R, Oda Y, Morikura T, Miyata S. Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process. International Journal of Molecular Sciences. 2022; 23(13):7091. https://doi.org/10.3390/ijms23137091

Chicago/Turabian Style

Nishi, Ryota, Yudai Oda, Takashi Morikura, and Shogo Miyata. 2022. "Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process" International Journal of Molecular Sciences 23, no. 13: 7091. https://doi.org/10.3390/ijms23137091

APA Style

Nishi, R., Oda, Y., Morikura, T., & Miyata, S. (2022). Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process. International Journal of Molecular Sciences, 23(13), 7091. https://doi.org/10.3390/ijms23137091

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Effect of Compressive Stress in Tumor Microenvironment on Malignant Tumor Spheroid Invasion Process

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