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Article

Mathematical Modeling Reveals That Sucrose Regulates Leaf Senescence via Dynamic Sugar Signaling Pathways

1
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, 666 Wusu Street, Hangzhou 311300, China
3
Graduate School of Chinese Academy of Agricultural Science, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Iwona Morkunas, Philippe Jeandet, Mateusz Labudda, Magda Formela-Luboińska and Soulaiman Sakr
Int. J. Mol. Sci. 2022, 23(12), 6498; https://doi.org/10.3390/ijms23126498
Received: 6 April 2022 / Revised: 30 May 2022 / Accepted: 7 June 2022 / Published: 10 June 2022
Sucrose (Suc) accumulation is one of the key indicators of leaf senescence onset, but little is known about its regulatory role. Here, we found that application of high (120–150 mM) and low levels (60 mM) of Suc to young leaf (YL) and fully expanded leaf (FEL) discs, respectively, decreased chlorophyll content and maximum photosynthetic efficiency. Electrolyte leakage and malondialdehyde levels increased at high Suc concentrations (90–120 mM in YL and 60 and 150 mM in FEL discs). In FEL discs, the senescence-associated gene NtSAG12 showed a gradual increase in expression with increased Suc application; in contrast, in YL discs, NtSAG12 was upregulated with low Suc treatment (60 mM) but downregulated at higher levels of Suc. In YL discs, trehalose-6-phosphate (T6P) accumulated at a low half-maximal effective concentration (EC50) of Suc (1.765 mM). However, T6P levels declined as trehalose 6 phosphate synthase (TPS) content decreased, resulting in the maximum velocity of sucrose non-fermenting-1-related protein kinase (SnRK) and hexokinase (HXK) occurring at higher level of Suc. We therefore speculated that senescence was induced by hexose accumulation. In FEL discs, the EC50 of T6P occurred at a low concentration of Suc (0.9488 mM); T6P levels progressively increased with higher TPS content, which inhibited SnRK activity with a dissociation constant (Kd) of 0.001475 U/g. This confirmed that the T6P–SnRK complex induced senescence in detached FEL discs. View Full-Text
Keywords: sucrose concentration; sugar signaling dynamics; glucose; trehalose-6-phosphate; SnRK1; senescence sucrose concentration; sugar signaling dynamics; glucose; trehalose-6-phosphate; SnRK1; senescence
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MDPI and ACS Style

Asim, M.; Hussain, Q.; Wang, X.; Sun, Y.; Liu, H.; Khan, R.; Du, S.; Shi, Y.; Zhang, Y. Mathematical Modeling Reveals That Sucrose Regulates Leaf Senescence via Dynamic Sugar Signaling Pathways. Int. J. Mol. Sci. 2022, 23, 6498. https://doi.org/10.3390/ijms23126498

AMA Style

Asim M, Hussain Q, Wang X, Sun Y, Liu H, Khan R, Du S, Shi Y, Zhang Y. Mathematical Modeling Reveals That Sucrose Regulates Leaf Senescence via Dynamic Sugar Signaling Pathways. International Journal of Molecular Sciences. 2022; 23(12):6498. https://doi.org/10.3390/ijms23126498

Chicago/Turabian Style

Asim, Muhammad, Quaid Hussain, Xiaolin Wang, Yanguo Sun, Haiwei Liu, Rayyan Khan, Shasha Du, Yi Shi, and Yan Zhang. 2022. "Mathematical Modeling Reveals That Sucrose Regulates Leaf Senescence via Dynamic Sugar Signaling Pathways" International Journal of Molecular Sciences 23, no. 12: 6498. https://doi.org/10.3390/ijms23126498

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