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Article

Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus)

1
South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic
2
Laboratory of Gene Expression, Institute of Biotechnology—Biocev, Academy of Science of Czech Republic, 252 50 Vestec, Czech Republic
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Institute of Aquaculture and Protection of Waters, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Husova tř. 458/102, 370 05 České Budějovice, Czech Republic
4
Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Claudio Luparello
Int. J. Mol. Sci. 2022, 23(12), 6392; https://doi.org/10.3390/ijms23126392
Received: 12 April 2022 / Revised: 3 June 2022 / Accepted: 3 June 2022 / Published: 7 June 2022
(This article belongs to the Special Issue Molecular Aspects in Fish and Amphibian Reproduction and Development)
DNA damage during early life stages may have a negative effect on embryo development, inducing mortality and malformations that have long-lasting effects during adult life. Therefore, in the current study, we analyzed the effect of DNA damage induced by genotoxicants (camptothecin (CPT) and olaparib) at different stages of embryo development. The survival, DNA fragmentation, transcriptome, and proteome of the endangered sturgeon Acipenser ruthenus were analyzed. Sturgeons are non-model fish species that can provide new insights into the DNA damage response and embryo development. The transcriptomic and proteomic patterns changed significantly after exposure to genotoxicants in a stage-dependent manner. The results of this study indicate a correlation between phenotype formation and changes in transcriptomic and proteomic profiles. CPT and olaparib downregulated oxidative phosphorylation and metabolic pathways, and upregulated pathways involved in nucleotide excision repair, base excision repair, and homologous recombination. We observed the upregulated expression of zona pellucida sperm-binding proteins in all treatment groups, as well as the upregulation of several glycolytic enzymes. The analysis of gene expression revealed several markers of DNA damage response and adaptive stress response, which could be applied in toxicological studies on fish embryos. This study is the first complex analysis of the DNA damage response in endangered sturgeons. View Full-Text
Keywords: fish; sturgeon; embryo; DNA damage response; omics fish; sturgeon; embryo; DNA damage response; omics
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MDPI and ACS Style

Gazo, I.; Naraine, R.; Lebeda, I.; Tomčala, A.; Dietrich, M.; Franěk, R.; Pšenička, M.; Šindelka, R. Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus). Int. J. Mol. Sci. 2022, 23, 6392. https://doi.org/10.3390/ijms23126392

AMA Style

Gazo I, Naraine R, Lebeda I, Tomčala A, Dietrich M, Franěk R, Pšenička M, Šindelka R. Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus). International Journal of Molecular Sciences. 2022; 23(12):6392. https://doi.org/10.3390/ijms23126392

Chicago/Turabian Style

Gazo, Ievgeniia, Ravindra Naraine, Ievgen Lebeda, Aleš Tomčala, Mariola Dietrich, Roman Franěk, Martin Pšenička, and Radek Šindelka. 2022. "Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus)" International Journal of Molecular Sciences 23, no. 12: 6392. https://doi.org/10.3390/ijms23126392

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