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Article

Ancient Origins of Cytoskeletal Crosstalk: Spectraplakin-like Proteins Precede the Emergence of Cortical Microtubule Stabilization Complexes as Crosslinkers

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Laboratory for Biotechnology in Aquaculture, Division of Materials Chemistry, Ruđer Bošković Institute, 10000 Zagreb, Croatia
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Center of Excellence for Marine Bioprospecting (BioProCro), Ruđer Bošković Institute, 10000 Zagreb, Croatia
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Laboratory for Metabolism and Aging, Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia
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Laboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, 10000 Zagreb, Croatia
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Laboratory of Experimental Therapy, Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia
*
Authors to whom correspondence should be addressed.
Academic Editor: Malgorzata Kloc
Int. J. Mol. Sci. 2022, 23(10), 5594; https://doi.org/10.3390/ijms23105594
Received: 28 March 2022 / Revised: 12 May 2022 / Accepted: 12 May 2022 / Published: 17 May 2022
(This article belongs to the Section Biochemistry)
Adhesion between cells and the extracellular matrix (ECM) is one of the prerequisites for multicellularity, motility, and tissue specialization. Focal adhesions (FAs) are defined as protein complexes that mediate signals from the ECM to major components of the cytoskeleton (microtubules, actin, and intermediate filaments), and their mutual communication determines a variety of cellular processes. In this study, human cytoskeletal crosstalk proteins were identified by comparing datasets with experimentally determined cytoskeletal proteins. The spectraplakin dystonin was the only protein found in all datasets. Other proteins (FAK, RAC1, septin 9, MISP, and ezrin) were detected at the intersections of FAs, microtubules, and actin cytoskeleton. Homology searches for human crosstalk proteins as queries were performed against a predefined dataset of proteomes. This analysis highlighted the importance of FA communication with the actin and microtubule cytoskeleton, as these crosstalk proteins exhibit the highest degree of evolutionary conservation. Finally, phylogenetic analyses elucidated the early evolutionary history of spectraplakins and cortical microtubule stabilization complexes (CMSCs) as model representatives of the human cytoskeletal crosstalk. While spectraplakins probably arose at the onset of opisthokont evolution, the crosstalk between FAs and microtubules is associated with the emergence of metazoans. The multiprotein complexes contributing to cytoskeletal crosstalk in animals gradually gained in complexity from the onset of metazoan evolution. View Full-Text
Keywords: cytoskeletal crosstalk; spectraplakin; cortical microtubule stabilization complex; focal adhesion; actin; microtubule; intermediate filaments; evolution; dystonin cytoskeletal crosstalk; spectraplakin; cortical microtubule stabilization complex; focal adhesion; actin; microtubule; intermediate filaments; evolution; dystonin
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MDPI and ACS Style

Paradžik, T.; Podgorski, I.I.; Vojvoda Zeljko, T.; Paradžik, M. Ancient Origins of Cytoskeletal Crosstalk: Spectraplakin-like Proteins Precede the Emergence of Cortical Microtubule Stabilization Complexes as Crosslinkers. Int. J. Mol. Sci. 2022, 23, 5594. https://doi.org/10.3390/ijms23105594

AMA Style

Paradžik T, Podgorski II, Vojvoda Zeljko T, Paradžik M. Ancient Origins of Cytoskeletal Crosstalk: Spectraplakin-like Proteins Precede the Emergence of Cortical Microtubule Stabilization Complexes as Crosslinkers. International Journal of Molecular Sciences. 2022; 23(10):5594. https://doi.org/10.3390/ijms23105594

Chicago/Turabian Style

Paradžik, Tina, Iva I. Podgorski, Tanja Vojvoda Zeljko, and Mladen Paradžik. 2022. "Ancient Origins of Cytoskeletal Crosstalk: Spectraplakin-like Proteins Precede the Emergence of Cortical Microtubule Stabilization Complexes as Crosslinkers" International Journal of Molecular Sciences 23, no. 10: 5594. https://doi.org/10.3390/ijms23105594

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