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Article

Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro

1
Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, Poland
2
Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, Poland
3
Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), 02-787 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Considered the senior author.
Academic Editor: Isabella Russo
Int. J. Mol. Sci. 2022, 23(10), 5504; https://doi.org/10.3390/ijms23105504
Received: 14 April 2022 / Revised: 11 May 2022 / Accepted: 12 May 2022 / Published: 14 May 2022
(This article belongs to the Special Issue Recent Advances in Biological Functions of Platelet)
Eukaryotic and prokaryotic cells in physiological and pathological conditions form membrane-bound extracellular vesicles, known as EVs. The ability of these submicron structures to transfer their cargoes (miRNA, DNA, protein, cytokines, receptors, etc.) into recipient cells is described. Recent data revealed that platelet-derived extracellular vesicles (PEVs) crosstalk promotes cancer growth and metastasis formation. Moreover, they exert immunosuppressive activities on phagocytes. This EV subpopulation is the most abundant amongst all types in circulation. According to the authors’ best knowledge, there is no information regarding the impact of PEVs on canine lymphocytes. The aim of this study was to evaluate the influence of PEVs on lymphocyte proliferation, phenotype and cytokine production in vitro. In the study, it was demonstrated (i) that PEVs interact differently with lymphocyte subsets and are preferentially associated with T-lymphocytes PBMC, while (ii) they are rarely detected in association with B-lymphocytes, and there is evidence that (iii) PEV uptake is observed after 7 h of co-culturing with lymphocytes. In addition, obtained data support the notion that PEVs do not influence in vitro lymphocyte proliferation, differentiation and cytokine production in a canine model. View Full-Text
Keywords: extracellular vesicles; exosomes; platelets; lymphocytes; dog; T-cells; B-cells; proliferation; IFN-gamma; IL-17 extracellular vesicles; exosomes; platelets; lymphocytes; dog; T-cells; B-cells; proliferation; IFN-gamma; IL-17
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MDPI and ACS Style

Żmigrodzka, M.; Witkowska-Piłaszewicz, O.; Pingwara, R.; Winnicka, A. Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro. Int. J. Mol. Sci. 2022, 23, 5504. https://doi.org/10.3390/ijms23105504

AMA Style

Żmigrodzka M, Witkowska-Piłaszewicz O, Pingwara R, Winnicka A. Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro. International Journal of Molecular Sciences. 2022; 23(10):5504. https://doi.org/10.3390/ijms23105504

Chicago/Turabian Style

Żmigrodzka, Magdalena, Olga Witkowska-Piłaszewicz, Rafał Pingwara, and Anna Winnicka. 2022. "Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro" International Journal of Molecular Sciences 23, no. 10: 5504. https://doi.org/10.3390/ijms23105504

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