Early Life Stress Alters Expression of Glucocorticoid Stress Response Genes and Trophic Factor Transcripts in the Rodent Basal Ganglia
Abstract
:1. Introduction
2. Results
2.1. Expression of Stress-Related Factors in the Substantia Nigra, Ventral Tegmental Area and Dorsal and Ventral Striatum
2.1.1. Effects of Early Life Stress on Expression of Stress-Related Transcripts, Seen in Both Females and Males
2.1.2. Sex Differences in the Effects of Early Life Stress on Expression of Stress-Related Transcripts
2.2. Expression of Bdnf Transcripts in the Substantia Nigra, Ventral Tegmental Area and Dorsal and Ventral Striatum
2.2.1. Effects of Early Life Stress on Expression of Bdnf Transcripts, Seen in Both Females and Males
2.2.2. Sex Differences in the Effects of Early Life Stress on Expression of Bdnf Transcripts
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. RNA Extraction
4.3. cDNA Synthesis
4.4. Quantitative Polymerase Chain Reaction (qPCR)
4.5. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Transcript | Outliers (Removed from Analysis) | N (after Removing Outliers) | ANOVA (Early Life Stress) | ANOVA (Sex) | ANOVA (Early Life Stress × Sex Interaction) |
---|---|---|---|---|---|
Substantia Nigra | |||||
Nr3c1 (GR) | - | 46 | F(1,42) = 3.78, p = 0.06 | F(1,42) = 0.17, p = 0.68 | F(1,42) = 0.03, p = 0.87 |
Fkbp5 | Stress (1) | 45 | F(1,41) = 0.70, p = 0.41 | F(1,41) = 4.17, p = 0.05 | F(1,41) = 6.33, p = 0.02 |
Fkbp4 | Control (1) | 45 | F(1,41) = 2.70, p = 0.11 | F(1,41) = 6.38, p = 0.02 | F(1,41) = 0.12, p = 0.73 |
Ptges3 | Control (3) | 43 | F(1,39) = 1.41, p = 0.24 | F(1,39) = 2.33, p = 0.14 | F(1,39) = 0.40, p = 0.53 |
Bag1 | - | 46 | F(1,42) = 2.18, p = 0.15 | F(1,42) = 1.16, p = 0.29 | F(1,42) = 1.19, p = 0.28 |
VTA | |||||
Nr3c1 (GR) | - | 46 | F(1,42) = 1.64, p = 0.21 | F(1,42) = 0.19, p = 0.67 | F(1,42) = 7.87, p= 0.008 |
Fkbp5 | Stress (2) | 44 | F(1,40) = 7.27, p = 0.01 | F(1,40) = 0.40, p = 0.53 | F(1,40) = 0.16, p = 0.69 |
Fkbp4 | Stress (1) | 45 | F(1,41) = 0.11, p = 0.75 | F(1,41) = 0.03, p = 0.85 | F(1,41) = 0.02, p = 0.90 |
Ptges3 | - | 46 | F(1,42) = 4.24, p = 0.05 | F(1,42) = 1.20, p = 0.28 | F(1,42) = 1.13, p = 0.29 |
Bag1 | - | 46 | F(1,42) = 10.25, p = 0.003 | F(1,42) = 0.82, p = 0.37 | F(1,42) = 7.66, p = 0.008 |
Dorsal Striatum | |||||
Nr3c1 (GR) | - | 46 | F(1,42) = 0.71, p = 0.40 | F(1,42) = 0.007, p = 0.93 | F(1,42) = 0.90, p = 0.35 |
Fkbp5 | - | 46 | F(1,42) = 1.28, p = 0.27 | F(1,42) = 0.36, p = 0.55 | F(1,42) = 0.03, p = 0.86 |
Fkbp4 | - | 46 | F(1,42) = 1.80, p = 0.19 | F(1,42) = 7.55, p = 0.009 | F(1,42) = 0.23, p = 0.63 |
Ptges3 | Stress (1) | 45 | F(1,41) = 0.28, p = 0.60 | F(1,41) = 0.001, p = 0.98 | F(1,41) = 0.03, p = 0.86 |
Bag1 * | - | 46 | F(1,42) = 0.23, p = 0.64 | F(1,42) = 0.08, p = 0.78 | F(1,42) = 2.07, p = 0.16 |
Ventral Striatum | |||||
Nr3c1 (GR) | - | 46 | F(1,42) = 0.009, p= 0.93 | F(1,42) = 0.04, p = 0.85 | F(1,42) = 2.78, p = 0.10 |
Fkbp5 | - | 46 | F(1,42) = 1.39, p = 0.25 | F(1,42) = 0.76, p = 0.39 | F(1,42) = 0.14, p = 0.72 |
Fkbp4 | Control (1) | 45 | F(1,41) = 0.001, p = 0.98 | F(1,41) = 1.77, p = 0.19 | F(1,41) = 1.78, p = 0.19 |
Ptges3 | Stress (2) | 44 | F(1,40) = 1.23, p = 0.28 | F(1,40) = 0.67, p = 0.42 | F(1,40) = 0.11, p = 0.74 |
Bag1 | - | 46 | F(1,42) = 2.60, p = 0.11 | F(1,42) = 0.25, p = 0.62 | F(1,42) = 0.32, p = 0.57 |
Transcripts | Outliers (Removed from Analyses) | N (after Removing Outliers) | ANOVA (Early Life Stress) | ANOVA (Sex) | ANOVA (Early Life Stress × Sex Interaction) |
---|---|---|---|---|---|
Substantia Nigra | |||||
Bdnf I | - | 46 | F(1,42) = 0.34, p = 0.56 | F(1,42) = 21.51, p < 0.001 | F(1,42) = 1.86, p = 0.18 |
Bdnf IIA | - | 46 | F(1,42) = 10.93, p = 0.002 | F(1,42) = 1.88, p = 0.18 | F(1,42) = 0.45, p = 0.50 |
Bdnf IIC | - | 46 | F(1,42) = 0.32, p = 0.58 | F(1,42) = 8.52, p = 0.006 | F(1,42) = 0.005, p = 0.94 |
Bdnf III | - | 46 | F(1,42) = 22.54, p < 0.001 | F(1,42) = 0.41, p = 0.53 | F(1,42) = 0.41, p = 0.52 |
Bdnf IV | - | 46 | F(1,42) = 0.51, p = 0.48 | F(1,42) = 3.11, p = 0.09 | F(1,42) = 2.28, p = 0.14 |
Bdnf VI | - | 46 | F(1,42) = 0.06, p = 0.82 | F(1,42) = 7.12, p = 0.01 | F(1,42) = 0.27, p = 0.60 |
(VTA | |||||
Bdnf I * | - | 46 | F(1,42) = 2.02, p = 0.16 | F(1,42) = 10.46, p = 0.002 | F(1,42) = 1.64, p = 0.21 |
Bdnf IIA | - | 46 | F(1,42) = 11.61, p = 0.001 | F(1,42) = 0.65, p = 0.43 | F(1,42) = 0.91, p = 0.34 |
Bdnf IIC | - | 46 | F(1,42) = 1.51, p = 0.23 | F(1,42) = 4.08, p = 0.05 | F(1,42) = 1.05, p = 0.31 |
Bdnf III | - | 46 | F(1,42) = 11.18, p = 0.002 | F(1,42) = 1.25, p = 0.27 | F(1,42) = 0.17, p = 0.69 |
Bdnf IV | Stress (1) | 45 | F(1,41) = 1.59, p = 0.21 | F(1,41) = 1.79, p = 0.19 | F(1,41) = 2.58, p = 0.12 |
Bdnf VI | Stress (1) | 45 | F(1,41) = 0.89, p = 0.35 | F(1,41) = 0.41, p = 0.53 | F(1,41) = 3.17, p = 0.08 |
Dorsal Striatum | |||||
Bdnf I * | Stress (1) | 45 | F(1,41) = 0.27, p = 0.61 | F(1,41) = 0.19, p = 0.67 | F(1,41) = 1.02, p = 0.32 |
Bdnf IIA | Stress (3), Control (1) | 42 | F(1,38) = 0.22, p = 0.65 | F(1,38) = 0.09, p = 0.77 | F(1,38) = 0.32, p = 0.57 |
Bdnf IIC | Stress (1), Control (1) | 44 | F(1,40) = 0.05, p = 0.83 | F(1,40) = 1.97, p = 0.17 | F(1,40) = 0.87, p = 0.36 |
Bdnf III | Stress (1) | 45 | F(1,41) = 0.45, p = 0.51 | F(1,41) = 1.20, p = 0.28 | F(1,41) = 1.61, p = 0.21 |
Bdnf IV | Stress (2) | 44 | F(1,40) = 0.02, p = 0.89 | F(1,40) = 1.19, p = 0.28 | F(1,40) = 0.98, p = 0.33 |
Bdnf VI* | Stress (1) | 45 | F(1,41) = 0.28, p = 0.60 | F(1,41) = 2.58, p = 0.12 | F(1,41) = 0.57, p = 0.45 |
Ventral Striatum | |||||
Bdnf I | Stress (2) | 44 | F(1,40) = 10.08, p = 0.003 | F(1,40) = 1.36, p = 0.25 | F(1,40) = 1.22, p = 0.28 |
Bdnf IIA * | Stress (1) | 45 | F(1,41) = 5.48, p = 0.02 | F(1,41) < 0.001, p = 0.98 | F(1,41) = 0.14, p = 0.72 |
Bdnf IIC | Stress (1) | 45 | F(1,41) = 6.48, p = 0.02 | F(1,41) = 0.18, p = 0.68 | F(1,41) = 0.05, p = 0.83 |
Bdnf III | Stress (2) | 44 | F(1,40) = 3.11, p = 0.09 | F(1,40) = 0.79, p = 0.38 | F(1,40) = 0.07, p = 0.80 |
Bdnf IV * | - | 46 | F(1,42) = 6.26, p = 0.02 | F(1,42) = 2.09, p = 0.16 | F(1,42) = 0.24, p = 0.63 |
Bdnf VI | Stress (1) | 45 | F(1,41) = 7.43, p = 0.009 | F(1,41) = 0.76, p = 0.39 | F(1,41) = 0.84, p = 0.37 |
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Tran, C.H.; Shannon Weickert, C.; Weickert, T.W.; Sinclair, D. Early Life Stress Alters Expression of Glucocorticoid Stress Response Genes and Trophic Factor Transcripts in the Rodent Basal Ganglia. Int. J. Mol. Sci. 2022, 23, 5333. https://doi.org/10.3390/ijms23105333
Tran CH, Shannon Weickert C, Weickert TW, Sinclair D. Early Life Stress Alters Expression of Glucocorticoid Stress Response Genes and Trophic Factor Transcripts in the Rodent Basal Ganglia. International Journal of Molecular Sciences. 2022; 23(10):5333. https://doi.org/10.3390/ijms23105333
Chicago/Turabian StyleTran, Cynthia Haidee, Cynthia Shannon Weickert, Thomas Wesley Weickert, and Duncan Sinclair. 2022. "Early Life Stress Alters Expression of Glucocorticoid Stress Response Genes and Trophic Factor Transcripts in the Rodent Basal Ganglia" International Journal of Molecular Sciences 23, no. 10: 5333. https://doi.org/10.3390/ijms23105333