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Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis

Food Biochemistry Group, Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, C/Eduardo Cabello, 6, CP 36208 Vigo, Spain
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Academic Editor: Mike Barbeck
Int. J. Mol. Sci. 2022, 23(1), 32; https://doi.org/10.3390/ijms23010032
Received: 16 November 2021 / Revised: 16 December 2021 / Accepted: 19 December 2021 / Published: 21 December 2021
(This article belongs to the Section Materials Science)
High molecular weight (Mw) collagen hydrolysates have been demonstrated to produce a higher synthesis of collagen type I mRNA. Mw determination is a key factor maximizing the effect of collagen hydrolysates on collagen type I synthesis by fibroblasts. This work aimed to achieve a high average Mw in Blue Shark Collagen Hydrolysate, studying different hydrolysis parameters by GPC-LS analysis and testing its effect on mRNA Type I collagen expression. Analysis revealed differences in blue shark collagen hydrolysates Mw depending on hydrolysis conditions. Papain leads to obtaining a significantly higher Mw hydrolysate than Alcalase at different times of hydrolysis and at different enzyme/substrate ratios. Besides, the time of the hydrolysis factor is more determinant than the enzyme/substrate ratio factor for obtaining a higher or lower hydrolysate Mw when using Papain as the enzyme. Contrary, Alcalase hydrolysates resulted in similar Mw with no significant differences between different conditions of hydrolysis assayed. Blue shark collagen hydrolysate showing the highest Mw showed neither cytotoxic nor proliferation effect on fibroblast cell culture. Besides, it exhibited an increasing effect on both mRNA expression and pro-collagen I production. View Full-Text
Keywords: fish by-products; collagen; fish protein hydrolysates; molecular weight; GPC-LS; fibroblast; mRNA collagen expression; pro-collagen fish by-products; collagen; fish protein hydrolysates; molecular weight; GPC-LS; fibroblast; mRNA collagen expression; pro-collagen
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MDPI and ACS Style

Blanco, M.; Sanz, N.; Sánzhez, A.C.; Correa, B.; Pérez-Martín, R.I.; Sotelo, C.G. Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis. Int. J. Mol. Sci. 2022, 23, 32. https://doi.org/10.3390/ijms23010032

AMA Style

Blanco M, Sanz N, Sánzhez AC, Correa B, Pérez-Martín RI, Sotelo CG. Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis. International Journal of Molecular Sciences. 2022; 23(1):32. https://doi.org/10.3390/ijms23010032

Chicago/Turabian Style

Blanco, María, Noelia Sanz, Ana C. Sánzhez, Begoña Correa, Ricardo I. Pérez-Martín, and Carmen G. Sotelo. 2022. "Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis" International Journal of Molecular Sciences 23, no. 1: 32. https://doi.org/10.3390/ijms23010032

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