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Article

Hot Spot Mutagenesis Improves the Functional Expression of Unique Mammalian Odorant Receptors

1
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
2
Instrumentation Analysis Center, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan
3
Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan
4
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Masoud Jelokhani-Niaraki and Edgar Soria-Gomez
Int. J. Mol. Sci. 2022, 23(1), 277; https://doi.org/10.3390/ijms23010277
Received: 19 November 2021 / Revised: 15 December 2021 / Accepted: 24 December 2021 / Published: 28 December 2021
(This article belongs to the Special Issue Membrane Proteins: Structure, Function and Motion)
Vertebrate animals detect odors through olfactory receptors (ORs), members of the G protein-coupled receptor (GPCR) family. Due to the difficulty in the heterologous expression of ORs, studies of their odor molecule recognition mechanisms have progressed poorly. Functional expression of most ORs in heterologous cells requires the co-expression of their chaperone proteins, receptor transporting proteins (RTPs). Yet, some ORs were found to be functionally expressed without the support of RTP (RTP-independent ORs). In this study, we investigated whether amino acid residues highly conserved among RTP-independent ORs improve the functional expression of ORs in heterologous cells. We found that a single amino acid substitution at one of two sites (NBW3.39 and 3.43) in their conserved residues (E and L, respectively) significantly improved the functional expression of ORs in heterologous cells. E3.39 and L3.43 also enhanced the membrane expression of RTP-dependent ORs in the absence of RTP. These changes did not alter the odorant responsiveness of the tested ORs. Our results showed that specific sites within transmembrane domains regulate the membrane expression of some ORs. View Full-Text
Keywords: odorant receptor; chemosensory; membrane traffic; heterologous expression odorant receptor; chemosensory; membrane traffic; heterologous expression
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MDPI and ACS Style

Fukutani, Y.; Nakamura, Y.; Muto, N.; Miyanaga, S.; Kanemaki, R.; Ikegami, K.; Noguchi, K.; Ohsawa, I.; Matsunami, H.; Yohda, M. Hot Spot Mutagenesis Improves the Functional Expression of Unique Mammalian Odorant Receptors. Int. J. Mol. Sci. 2022, 23, 277. https://doi.org/10.3390/ijms23010277

AMA Style

Fukutani Y, Nakamura Y, Muto N, Miyanaga S, Kanemaki R, Ikegami K, Noguchi K, Ohsawa I, Matsunami H, Yohda M. Hot Spot Mutagenesis Improves the Functional Expression of Unique Mammalian Odorant Receptors. International Journal of Molecular Sciences. 2022; 23(1):277. https://doi.org/10.3390/ijms23010277

Chicago/Turabian Style

Fukutani, Yosuke, Yuko Nakamura, Nonoko Muto, Shunta Miyanaga, Reina Kanemaki, Kentaro Ikegami, Keiichi Noguchi, Ikuroh Ohsawa, Hiroaki Matsunami, and Masafumi Yohda. 2022. "Hot Spot Mutagenesis Improves the Functional Expression of Unique Mammalian Odorant Receptors" International Journal of Molecular Sciences 23, no. 1: 277. https://doi.org/10.3390/ijms23010277

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