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Article

First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique

by
Linan He
1,
Jinqiao Shi
1,
Zhibo Zhao
1,2,
Fei Ran
1,
Feixu Mo
1,
Youhua Long
1,2,*,
Xianhui Yin
1,2,
Wenzhi Li
1,
Tingting Chen
1 and
Jia Chen
1
1
Research Center for Engineering Technology of Kiwifruit, Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang 550025, China
2
Teaching Experimental Field of Guizhou University, Guizhou University, Guiyang 550025, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(1), 207; https://doi.org/10.3390/ijms23010207
Submission received: 10 November 2021 / Revised: 19 December 2021 / Accepted: 22 December 2021 / Published: 24 December 2021
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Kiwifruit is moderately sweet and sour and quite popular among consumers; it has been widely planted in some areas of the world. In 2019, the crown gall disease of kiwifruit was discovered in the main kiwifruit-producing area of Guizhou Province, China. This disease can weaken and eventually cause the death of the tree. The phylogeny, morphological and biological characteristics of the bacteria were described, and were related to diseases. The pathogenicity of this species follows the Koch hypothesis, confirming that A. fabacearum is the pathogen of crown gall disease of kiwifruit in China. In this study, Loop-mediated isothermal amplification (LAMP) analysis for genome-specific gene sequences was developed for the specific detection of A. fabacearum. The detection limit of the LAMP method is 5 × 10−7 ng/μL, which has high sensitivity. At the same time, the amplified product is stained with SYBR Green I after the reaction is completed, so that the amplification can be detected with the naked eye. LAMP analysis detected the presence of A. fabacearum in the roots and soil samples of the infected kiwifruit plant. The proposed LAMP detection technology in this study offers the advantages of ease of operation, visibility of results, rapidity, accuracy and high sensitivity, making it suitable for the early diagnosis of crown gall disease of kiwifruit.
Keywords: crown gall; kiwifruit; A. fabacearum; LAMP crown gall; kiwifruit; A. fabacearum; LAMP

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MDPI and ACS Style

He, L.; Shi, J.; Zhao, Z.; Ran, F.; Mo, F.; Long, Y.; Yin, X.; Li, W.; Chen, T.; Chen, J. First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique. Int. J. Mol. Sci. 2022, 23, 207. https://doi.org/10.3390/ijms23010207

AMA Style

He L, Shi J, Zhao Z, Ran F, Mo F, Long Y, Yin X, Li W, Chen T, Chen J. First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique. International Journal of Molecular Sciences. 2022; 23(1):207. https://doi.org/10.3390/ijms23010207

Chicago/Turabian Style

He, Linan, Jinqiao Shi, Zhibo Zhao, Fei Ran, Feixu Mo, Youhua Long, Xianhui Yin, Wenzhi Li, Tingting Chen, and Jia Chen. 2022. "First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique" International Journal of Molecular Sciences 23, no. 1: 207. https://doi.org/10.3390/ijms23010207

APA Style

He, L., Shi, J., Zhao, Z., Ran, F., Mo, F., Long, Y., Yin, X., Li, W., Chen, T., & Chen, J. (2022). First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique. International Journal of Molecular Sciences, 23(1), 207. https://doi.org/10.3390/ijms23010207

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