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Article

Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse

1
Department of Anatomy, Albert Szent-Györgyi Faculty of Medicine, University of Szeged, Kossuth L. sgt. 38, H-6724 Szeged, Hungary
2
Department of Medical Chemistry, University of Szeged, Dóm tér. 8, H-6720 Szeged, Hungary
3
Professional Pedagogical Service of Csongrád-Csanád County, Űrhajós u. 4, H-6723 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Jarogniew J. Łuszczki
Int. J. Mol. Sci. 2022, 23(1), 204; https://doi.org/10.3390/ijms23010204
Received: 5 December 2021 / Revised: 21 December 2021 / Accepted: 23 December 2021 / Published: 24 December 2021
(This article belongs to the Special Issue Advances in Epilepsy and Antiepileptic Drugs)
The present experiments reveal the alterations of the hippocampal neuronal populations in chronic epilepsy. The mice were injected with a single dose of pilocarpine. They had status epilepticus and spontaneously recurrent motor seizures. Three months after pilocarpine treatment, the animals were investigated with the Barnes maze to determine their learning and memory capabilities. Their hippocampi were analyzed 2 weeks later (at 3.5 months) with standard immunohistochemical methods and cell counting. Every animal displayed hippocampal sclerosis. The neuronal loss was evaluated with neuronal-N immunostaining, and the activation of the microglia was measured with Iba1 immunohistochemistry. The neuropeptide Y, parvalbumin, and calretinin immunoreactive structures were qualitatively and quantitatively analyzed in the hippocampal formation. The results were compared statistically to the results of the control mice. We detected neuronal loss and strongly activated microglia populations. Neuropeptide Y was significantly upregulated in the sprouting axons. The number of parvalbumin- and calretinin-containing interneurons decreased significantly in the Ammon’s horn and dentate gyrus. The epileptic animals displayed significantly worse learning and memory functions. We concluded that degeneration of the principal neurons, a numerical decrease of PV-containing GABAergic neurons, and strong peptidergic axonal sprouting were responsible for the loss of the hippocampal learning and memory functions. View Full-Text
Keywords: hippocampus; epilepsy; pilocarpine; mouse; neuropeptide-Y; parvalbumin; calretinin; learning hippocampus; epilepsy; pilocarpine; mouse; neuropeptide-Y; parvalbumin; calretinin; learning
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MDPI and ACS Style

Mátyás, A.; Borbély, E.; Mihály, A. Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse. Int. J. Mol. Sci. 2022, 23, 204. https://doi.org/10.3390/ijms23010204

AMA Style

Mátyás A, Borbély E, Mihály A. Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse. International Journal of Molecular Sciences. 2022; 23(1):204. https://doi.org/10.3390/ijms23010204

Chicago/Turabian Style

Mátyás, Adrienne, Emőke Borbély, and András Mihály. 2022. "Hippocampal Sclerosis in Pilocarpine Epilepsy: Survival of Peptide-Containing Neurons and Learning and Memory Disturbances in the Adult NMRI Strain Mouse" International Journal of Molecular Sciences 23, no. 1: 204. https://doi.org/10.3390/ijms23010204

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