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Article

Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons

1
Department of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics, Karolinska Institutet, 171 64 Solna, Sweden
2
SciLifeLab, Department of Applied Physics, Biophysics, Royal Institute of Technology, 171 65 Solna, Sweden
3
Theme Inflammation and Aging, Karolinska University Hospital, 141 86 Huddinge, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Marco Diociaiuti
Int. J. Mol. Sci. 2021, 22(9), 4530; https://doi.org/10.3390/ijms22094530
Received: 15 March 2021 / Revised: 22 April 2021 / Accepted: 24 April 2021 / Published: 26 April 2021
Amyloid β-peptide (Aβ) oligomerization is believed to contribute to the neuronal dysfunction in Alzheimer disease (AD). Despite decades of research, many details of Aβ oligomerization in neurons still need to be revealed. Förster resonance energy transfer (FRET) is a simple but effective way to study molecular interactions. Here, we used a confocal microscope with a sensitive Airyscan detector for FRET detection. By live cell FRET imaging, we detected Aβ42 oligomerization in primary neurons. The neurons were incubated with fluorescently labeled Aβ42 in the cell culture medium for 24 h. Aβ42 were internalized and oligomerized in the lysosomes/late endosomes in a concentration-dependent manner. Both the cellular uptake and intracellular oligomerization of Aβ42 were significantly higher than for Aβ40. These findings provide a better understanding of Aβ42 oligomerization in neurons. View Full-Text
Keywords: amyloid β-peptide; oligomerization; aggregation; FRET amyloid β-peptide; oligomerization; aggregation; FRET
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MDPI and ACS Style

Gao, Y.; Wennmalm, S.; Winblad, B.; Schedin-Weiss, S.; Tjernberg, L.O. Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons. Int. J. Mol. Sci. 2021, 22, 4530. https://doi.org/10.3390/ijms22094530

AMA Style

Gao Y, Wennmalm S, Winblad B, Schedin-Weiss S, Tjernberg LO. Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons. International Journal of Molecular Sciences. 2021; 22(9):4530. https://doi.org/10.3390/ijms22094530

Chicago/Turabian Style

Gao, Yang, Stefan Wennmalm, Bengt Winblad, Sophia Schedin-Weiss, and Lars O. Tjernberg. 2021. "Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons" International Journal of Molecular Sciences 22, no. 9: 4530. https://doi.org/10.3390/ijms22094530

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