Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis, Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions
Abstract
:1. Introduction
2. Results
2.1. Identification of CAT Genes Family in Rapeseed
2.2. Phylogenetic Relationships of CAT Genes
2.3. Synteny Analysis of CAT Genes
2.4. Analysis of BnCAT Gene Structures and Conserved Motifs
2.5. Cis-Elements in Promoters of BnCAT Genes
2.6. Genome-Wide Analysis of miRNA Targeting BnCAT Genes
2.7. Functional Annotation Analysis of BnCAT Genes
2.8. Expression Profiling of BnCAT Genes in Various Tissues
2.9. Expression Profiles of BnCAT Genes under Phytohormones and Abiotic Stress Conditions
3. Discussion
3.1. Characterization of CAT Gene Family in Rapeseed
3.2. The Critical Role of CAT Genes in ROS Metabolism, Hormone, and Abiotic Stress Responses
3.3. Expression Pattern of CAT Genes in Various Tissues
3.4. miRNA: Key Players in the Regulation of Stress Responses
4. Materials and Methods
4.1. Identification of CAT Family Genes
4.2. Characterization of CAT Family Genes
4.3. Phylogenetic Tree and Synteny Analysis of BnCAT Family Proteins
4.4. Cis-Elements Analysis in the BnCAT Gene Promoters
4.5. Prediction of Putative miRNA Targeting BnCAT Genes and Functional Annotation Analysis
4.6. Expression Analysis of BnCAT Genes in Different Tissues
4.7. Plant Material and Stress Conditions
4.8. RNA Extraction and qRT-PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Gene Name | Genomic Position (bp) | Gene Length (bp) | CDS Length (bp) | Exon | Protein Length (Amino Acids) | Molecular Weight (kDa) | Isoelectric Point (pI) | Sub-Cellular Localization |
---|---|---|---|---|---|---|---|---|---|
BnaA01T0032600ZS | BnCAT1 | A01:1801796-1803991 + | 2195 | 1488 | 4 | 495 | 57.30 | 6.5 | Cytoskeleton |
BnaA03T0547900ZS | BnCAT2 | A03:31026573-31028922 − | 2349 | 1479 | 5 | 492 | 56.84 | 6.63 | Peroxisome |
BnaA06T0144600ZS | BnCAT3 | A06:8685726-8691515 + | 5789 | 2925 | 12 | 974 | 112.30 | 6.78 | Peroxisome |
BnaA07T0132000ZS | BnCAT4 | A07:17121532-17124087 − | 2555 | 1479 | 5 | 492 | 56.90 | 6.83 | Cytoplasm |
BnaA07T0132100ZS | BnCAT5 | A07:17124704-17130749 − | 6045 | 1671 | 6 | 556 | 64.83 | 8.42 | Chloroplast |
BnaA08T0131200ZS | BnCAT6 | A08:18424311-18426301 + | 1990 | 1470 | 4 | 489 | 565.40 | 6.9 | Peroxisome |
BnaA08T0247000ZS | BnCAT7 | A08:25020319-25023539 − | 3220 | 1479 | 5 | 492 | 56.80 | 7.69 | Peroxisome |
BnaC03T0740800ZS | BnCAT8 | C03:71851456-71853466 + | 2010 | 1470 | 4 | 489 | 56.52 | 6.77 | Peroxisome |
BnaC05T0176000ZS | BnCAT9 | C05:11786858-11795441 + | 8583 | 2949 | 12 | 982 | 113.09 | 6.94 | Mitochondrion |
BnaC07T0194100ZS | BnCAT10 | C07:32771906-32781676 − | 9770 | 3123 | 15 | 1040 | 120.71 | 7.34 | Chloroplast |
BnaC07T0524400ZS | BnCAT11 | C07:59429784-59432167 − | 2383 | 1479 | 4 | 492 | 56.78 | 6.63 | Peroxisome |
BnaC08T0260800ZS | BnCAT12 | C08:34921906-34925039 + | 3133 | 1479 | 5 | 492 | 56.80 | 7.69 | Peroxisome |
Bnascaffold0025T0038400ZS | BnCAT13 | 0025:3787780-3790910 − | 3130 | 1434 | 5 | 477 | 55.19 | 7.72 | Peroxisome |
Bnascaffold0026T0027200ZS | BnCAT14 | 0026:2854478-2857610 + | 3132 | 1479 | 5 | 492 | 56.80 | 7.69 | Peroxisome |
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Raza, A.; Su, W.; Gao, A.; Mehmood, S.S.; Hussain, M.A.; Nie, W.; Lv, Y.; Zou, X.; Zhang, X. Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis, Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions. Int. J. Mol. Sci. 2021, 22, 4281. https://doi.org/10.3390/ijms22084281
Raza A, Su W, Gao A, Mehmood SS, Hussain MA, Nie W, Lv Y, Zou X, Zhang X. Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis, Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions. International Journal of Molecular Sciences. 2021; 22(8):4281. https://doi.org/10.3390/ijms22084281
Chicago/Turabian StyleRaza, Ali, Wei Su, Ang Gao, Sundas Saher Mehmood, Muhammad Azhar Hussain, Wenlong Nie, Yan Lv, Xiling Zou, and Xuekun Zhang. 2021. "Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis, Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions" International Journal of Molecular Sciences 22, no. 8: 4281. https://doi.org/10.3390/ijms22084281