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Article

Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach

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iMed., ULisboa, Research Institute for Medicines, Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal
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Centro de Química Estrutural, Instituto Superior Técnico, Departamento de Engenharia Química, Universidade de Lisboa, 1049-001 Lisboa, Portugal
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Centro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
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Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, 1794-024 Lisboa, Portugal
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CBIOS-Research Center for Biosciences & Health Technologies, Universidade Lusófona de Humanidades e Tecnologias, 1794-024 Lisboa, Portugal
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Centro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
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Department of Biomedical Sciences, Faculty of Pharmacy, University of Alcalá, Campus Universitario, 28871 Alcalá de Henares, Spain
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IBEB, Biophysics and Biomedical Engineering, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
Academic Editor: David A. Hart
Int. J. Mol. Sci. 2021, 22(8), 4087; https://doi.org/10.3390/ijms22084087
Received: 6 March 2021 / Revised: 10 April 2021 / Accepted: 12 April 2021 / Published: 15 April 2021
(This article belongs to the Special Issue Therapeutic Polymers for Drug Delivery)
Insulin is a peptide hormone with many physiological functions, besides its use in diabetes treatment. An important role of insulin is related to the wound healing process—however, insulin itself is too sensitive to the external environment requiring the protective of a nanocarrier. Polymer-based nanoparticles can protect, deliver, and retain the protein in the target area. This study aims to produce and characterize a topical treatment for wound healing consisting of insulin-loaded poly-DL-lactide/glycolide (PLGA) nanoparticles. Insulin-loaded nanoparticles present a mean size of approximately 500 nm and neutral surface charge. Spherical shaped nanoparticles are observed by scanning electron microscopy and confirmed by atomic force microscopy. SDS-PAGE and circular dichroism analysis demonstrated that insulin preserved its integrity and secondary structure after the encapsulation process. In vitro release studies suggested a controlled release profile. Safety of the formulation was confirmed using cell lines, and cell viability was concentration and time-dependent. Preliminary safety in vivo assays also revealed promising results. View Full-Text
Keywords: wound healing; insulin; nanoparticles; PLGA; topical administration wound healing; insulin; nanoparticles; PLGA; topical administration
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MDPI and ACS Style

Quitério, M.; Simões, S.; Ascenso, A.; Carvalheiro, M.; Leandro, A.P.; Correia, I.; Viana, A.S.; Faísca, P.; Ascensão, L.; Molpeceres, J.; Gaspar, M.M.; Reis, C.P. Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach. Int. J. Mol. Sci. 2021, 22, 4087. https://doi.org/10.3390/ijms22084087

AMA Style

Quitério M, Simões S, Ascenso A, Carvalheiro M, Leandro AP, Correia I, Viana AS, Faísca P, Ascensão L, Molpeceres J, Gaspar MM, Reis CP. Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach. International Journal of Molecular Sciences. 2021; 22(8):4087. https://doi.org/10.3390/ijms22084087

Chicago/Turabian Style

Quitério, Maria, Sandra Simões, Andreia Ascenso, Manuela Carvalheiro, Ana P. Leandro, Isabel Correia, Ana S. Viana, Pedro Faísca, Lia Ascensão, Jesús Molpeceres, Maria M. Gaspar, and Catarina P. Reis 2021. "Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach" International Journal of Molecular Sciences 22, no. 8: 4087. https://doi.org/10.3390/ijms22084087

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