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Open AccessArticle

Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus

1
CHU de Québec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec, QC G1V 4G2, Canada
2
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3B 3M9, Canada
3
Division of Microbiology, Department of Laboratory Medicine & Molecular Diagnostics, Sunnybrook Health Sciences Centre, Toronto, ON M4N 3M5, Canada
4
Département de Microbiologie Médicale, Université du Manitoba, Winnipeg, MB R3E 0J9, Canada
5
CHUQ Research Center/CHUL Pavilion, 2705 Blvd Laurier, Room T1-65, Quebec, QC G1V 4G2, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Andreas Burkovski
Int. J. Mol. Sci. 2021, 22(7), 3792; https://doi.org/10.3390/ijms22073792
Received: 1 March 2021 / Revised: 27 March 2021 / Accepted: 30 March 2021 / Published: 6 April 2021
(This article belongs to the Special Issue Host–Pathogen Interaction 2.0)
Ebola virus (EBOV) is a virulent pathogen, notorious for inducing life-threatening hemorrhagic fever, that has been responsible for several outbreaks in Africa and remains a public health threat. Yet, its pathogenesis is still not completely understood. Although there have been numerous studies on host transcriptional response to EBOV, with an emphasis on the clinical features, the impact of EBOV infection on post-transcriptional regulatory elements, such as microRNAs (miRNAs), remains largely unexplored. MiRNAs are involved in inflammation and immunity and are believed to be important modulators of the host response to viral infection. Here, we have used small RNA sequencing (sRNA-Seq), qPCR and functional analyses to obtain the first comparative miRNA transcriptome (miRNome) of a human liver cell line (Huh7) infected with one of the following three EBOV strains: Mayinga (responsible for the first Zaire outbreak in 1976), Makona (responsible for the West Africa outbreak in 2013–2016) and the epizootic Reston (presumably innocuous to humans). Our results highlight specific miRNA-based immunity pathways and substantial differences between the strains beyond their clinical manifestation and pathogenicity. These analyses shed new light into the molecular signature of liver cells upon EBOV infection and reveal new insights into miRNA-based virus attack and host defense strategy. View Full-Text
Keywords: Ebola virus; microRNA; liver cell; transcriptome; small RNA sequencing Ebola virus; microRNA; liver cell; transcriptome; small RNA sequencing
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MDPI and ACS Style

Diallo, I.; Ho, J.; Laffont, B.; Laugier, J.; Benmoussa, A.; Lambert, M.; Husseini, Z.; Soule, G.; Kozak, R.; Kobinger, G.P.; Provost, P. Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus. Int. J. Mol. Sci. 2021, 22, 3792. https://doi.org/10.3390/ijms22073792

AMA Style

Diallo I, Ho J, Laffont B, Laugier J, Benmoussa A, Lambert M, Husseini Z, Soule G, Kozak R, Kobinger GP, Provost P. Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus. International Journal of Molecular Sciences. 2021; 22(7):3792. https://doi.org/10.3390/ijms22073792

Chicago/Turabian Style

Diallo, Idrissa; Ho, Jeffrey; Laffont, Benoit; Laugier, Jonathan; Benmoussa, Abderrahim; Lambert, Marine; Husseini, Zeinab; Soule, Geoff; Kozak, Robert; Kobinger, Gary P.; Provost, Patrick. 2021. "Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus" Int. J. Mol. Sci. 22, no. 7: 3792. https://doi.org/10.3390/ijms22073792

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