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Article

EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers

1
Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland
2
Department of Plant Physiology, Institute of Biology, Warsaw University of Life Sciences-SGGW (WULS-SGGW), Nowoursynowska 159 Street, 02-776 Warsaw, Poland
3
Department of Plant Physiology and Biotechnology, University of Gdańsk, 59 Wita Stwosza, 80-308 Gdańsk, Poland
4
Department of Plant Cytology and Embryology, University of Gdańsk, 59 Wita Stwosza, 80-308 Gdańsk, Poland
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Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdańsk, Abrahama 58, 80-307 Gdańsk, Poland
6
UMR DIADE, IRD Centre de Montpellier, Institut de Recherche pour le Développement, Université de Montpellier, 911 Avenue Agropolis BP 64501, 34394 CEDEX 5 Montpellier, France
*
Author to whom correspondence should be addressed.
Academic Editor: Silvia Coimbra
Int. J. Mol. Sci. 2021, 22(6), 3001; https://doi.org/10.3390/ijms22063001
Received: 2 February 2021 / Revised: 8 March 2021 / Accepted: 12 March 2021 / Published: 16 March 2021
(This article belongs to the Special Issue Signaling in Plant Reproduction)
Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells? Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ. EPIP stimulates the downstream elements of the pathway—HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation. The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase. A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism. Notably, the formation of a signaling molecule—phosphatidic acid is induced locally in EPIP-treated AZ. Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission. View Full-Text
Keywords: cell wall; crops; fatty acids; flower abscission zone; oxidative stress; yellow lupine cell wall; crops; fatty acids; flower abscission zone; oxidative stress; yellow lupine
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MDPI and ACS Style

Wilmowicz, E.; Kućko, A.; Pokora, W.; Kapusta, M.; Jasieniecka-Gazarkiewicz, K.; Tranbarger, T.J.; Wolska, M.; Panek, K. EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. Int. J. Mol. Sci. 2021, 22, 3001. https://doi.org/10.3390/ijms22063001

AMA Style

Wilmowicz E, Kućko A, Pokora W, Kapusta M, Jasieniecka-Gazarkiewicz K, Tranbarger TJ, Wolska M, Panek K. EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. International Journal of Molecular Sciences. 2021; 22(6):3001. https://doi.org/10.3390/ijms22063001

Chicago/Turabian Style

Wilmowicz, Emilia, Agata Kućko, Wojciech Pokora, Małgorzata Kapusta, Katarzyna Jasieniecka-Gazarkiewicz, Timothy J. Tranbarger, Magdalena Wolska, and Katarzyna Panek. 2021. "EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers" International Journal of Molecular Sciences 22, no. 6: 3001. https://doi.org/10.3390/ijms22063001

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