6-Azauridine Induces Autophagy-Mediated Cell Death via a p53- and AMPK-Dependent Pathway
Abstract
:1. Introduction
2. Results
2.1. 6-AZA Activates Autophagic Flux in Cancer Cells
2.2. 6-AZA Activates Lysosomal Function in Cancer Cells
2.3. 6-AZA Treatment Induces Autophagy-Mediated Cell Death
2.4. 6-AZA Shows Diverse Cytotoxicity to Various Cancer Cells
2.5. p53 and AMPK Affect Cellular Response to 6-AZA Treatment
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Cell Proliferation Assay
4.2. Western Blot
4.3. Immunofluorescence Staining and Confocal Microscopy
4.4. Flow Cytometry for Cell Cycle and Lysosome Analysis
4.5. Quantitative Reverse Transcription-PCR
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Cha, Y.-E.; Park, R.; Jang, M.; Park, Y.-I.; Yamamoto, A.; Oh, W.K.; Lee, E.-J.; Park, J. 6-Azauridine Induces Autophagy-Mediated Cell Death via a p53- and AMPK-Dependent Pathway. Int. J. Mol. Sci. 2021, 22, 2947. https://doi.org/10.3390/ijms22062947
Cha Y-E, Park R, Jang M, Park Y-I, Yamamoto A, Oh WK, Lee E-J, Park J. 6-Azauridine Induces Autophagy-Mediated Cell Death via a p53- and AMPK-Dependent Pathway. International Journal of Molecular Sciences. 2021; 22(6):2947. https://doi.org/10.3390/ijms22062947
Chicago/Turabian StyleCha, Yeo-Eun, Rackhyun Park, Minsu Jang, Yea-In Park, Ayane Yamamoto, Won Keun Oh, Eun-Ju Lee, and Junsoo Park. 2021. "6-Azauridine Induces Autophagy-Mediated Cell Death via a p53- and AMPK-Dependent Pathway" International Journal of Molecular Sciences 22, no. 6: 2947. https://doi.org/10.3390/ijms22062947