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Article

Analysis of blaCHDL Genes and Insertion Sequences Related to Carbapenem Resistance in Acinetobacter baumannii Clinical Strains Isolated in Warsaw, Poland

1
Department of Pharmaceutical Microbiology, Medical University of Warsaw, PL 02-097 Warsaw, Poland
2
Public Health England, National Infection Service, Porton Down, Salisbury SP4 0JG, UK
3
Department of Antibiotics and Microbiology, National Medicines Institute, PL 00-725 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Cristina Marzano
Int. J. Mol. Sci. 2021, 22(5), 2486; https://doi.org/10.3390/ijms22052486
Received: 8 February 2021 / Revised: 18 February 2021 / Accepted: 23 February 2021 / Published: 2 March 2021
(This article belongs to the Special Issue Multi-Drug Resistant Bacteria—A Global Problem)
Acinetobacter baumannii is an important cause of nosocomial infections worldwide. The elucidation of the carbapenem resistance mechanisms of hospital strains is necessary for the effective treatment and prevention of resistance gene transmission. The main mechanism of carbapenem resistance in A. baumannii is carbapenemases, whose expressions are affected by the presence of insertion sequences (ISs) upstream of blaCHDL genes. In this study, 61 imipenem-nonsusceptible A. baumannii isolates were characterized using phenotypic (drug-susceptibility profile using CarbaAcineto NP) and molecular methods. Pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) methods were utilized for the genotyping. The majority of isolates (59/61) carried one of the following acquired blaCHDL genes: blaOXA-24-like (39/59), ISAba1-blaOXA-23-like (14/59) or ISAba3-blaOXA-58-like (6/59). Whole genome sequence analysis of 15 selected isolates identified the following intrinsic blaOXA-66 (OXA-51-like; n = 15) and acquired class D β-lactamases (CHDLs): ISAba1-blaOXA-23 (OXA-23-like; n = 7), ISAba3-blaOXA-58-ISAba3 (OXA-58-like; n = 2) and blaOXA-72 (OXA-24-like; n = 6). The isolates were classified into 21 pulsotypes using PFGE, and the representative 15 isolates were found to belong to sequence type ST2 of the Pasteur MLST scheme from the global IC2 clone. The Oxford MLST scheme revealed the diversity among these studied isolates, and identified five sequence types (ST195, ST208, ST208/ST1806, ST348 and ST425). CHDL-type carbapenemases and insertion elements upstream of the blaCHDL genes were found to be widespread among Polish A. baumannii clinical isolates, and this contributed to their carbapenem resistance. View Full-Text
Keywords: multidrug resistance; carbapenem resistance; CHDL enzymes; insertion sequences; CarbAcineto NP test; PFGE; MLST multidrug resistance; carbapenem resistance; CHDL enzymes; insertion sequences; CarbAcineto NP test; PFGE; MLST
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MDPI and ACS Style

Słoczyńska, A.; Wand, M.E.; Tyski, S.; Laudy, A.E. Analysis of blaCHDL Genes and Insertion Sequences Related to Carbapenem Resistance in Acinetobacter baumannii Clinical Strains Isolated in Warsaw, Poland. Int. J. Mol. Sci. 2021, 22, 2486. https://doi.org/10.3390/ijms22052486

AMA Style

Słoczyńska A, Wand ME, Tyski S, Laudy AE. Analysis of blaCHDL Genes and Insertion Sequences Related to Carbapenem Resistance in Acinetobacter baumannii Clinical Strains Isolated in Warsaw, Poland. International Journal of Molecular Sciences. 2021; 22(5):2486. https://doi.org/10.3390/ijms22052486

Chicago/Turabian Style

Słoczyńska, Alicja, Matthew E. Wand, Stefan Tyski, and Agnieszka E. Laudy. 2021. "Analysis of blaCHDL Genes and Insertion Sequences Related to Carbapenem Resistance in Acinetobacter baumannii Clinical Strains Isolated in Warsaw, Poland" International Journal of Molecular Sciences 22, no. 5: 2486. https://doi.org/10.3390/ijms22052486

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