OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice
Abstract
:1. Introduction
2. Results
2.1. Identification of Cold Stress Mutant Oscbe1
2.2. OsCBE1 Negatively Regulates Stress Response and ABA Signaling
2.3. Expression of OsCBE1 Positively Related to Crop Productivity
2.4. Functional Role of OsCBE1 and In Silico Identification of the OsCBE1 Binding Partners
2.5. Physical Interaction of OsCBE1 with OsDDB1 and OsC3H32
2.6. Oleosin Genes and an ABA Responsive Gene Are Negatively Regulated by OsCBE1
3. Discussion
4. Materials and Methods
4.1. Plant Growth and Stress Tolerance Test
4.2. Analysis of Chlorophyll Content and Fluorescence
4.3. PCR and RT-PCR Analysis
4.4. Vector Construction
4.5. Generation of OsCBE1 Overexpression Lines
4.6. Germination Test
4.7. Evaluation of Agronomic Traits
4.8. In Silico Analysis
4.9. Co-IP Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Choi, J.; Lee, W.; An, G.; Kim, S.-R. OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice. Int. J. Mol. Sci. 2021, 22, 2487. https://doi.org/10.3390/ijms22052487
Choi J, Lee W, An G, Kim S-R. OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice. International Journal of Molecular Sciences. 2021; 22(5):2487. https://doi.org/10.3390/ijms22052487
Chicago/Turabian StyleChoi, Juyoung, Wonkyung Lee, Gynheung An, and Seong-Ryong Kim. 2021. "OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice" International Journal of Molecular Sciences 22, no. 5: 2487. https://doi.org/10.3390/ijms22052487