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Commentary

COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies

1
Medical Technology Research Centre, Anglia Ruskin University, Chelmsford CM1 1SQ, UK
2
RM Consulting, San Diego, CA 92037, USA
3
Shipley Consulting, Vancouver, WA 98682, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Luisa Barzon
Int. J. Mol. Sci. 2021, 22(5), 2459; https://doi.org/10.3390/ijms22052459
Received: 20 January 2021 / Revised: 24 February 2021 / Accepted: 24 February 2021 / Published: 28 February 2021
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use. View Full-Text
Keywords: COVID-19; SARS-CoV-2; molecular diagnostics; quantification cycle; RT-qPCR COVID-19; SARS-CoV-2; molecular diagnostics; quantification cycle; RT-qPCR
MDPI and ACS Style

Bustin, S.; Mueller, R.; Shipley, G.; Nolan, T. COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies. Int. J. Mol. Sci. 2021, 22, 2459. https://doi.org/10.3390/ijms22052459

AMA Style

Bustin S, Mueller R, Shipley G, Nolan T. COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies. International Journal of Molecular Sciences. 2021; 22(5):2459. https://doi.org/10.3390/ijms22052459

Chicago/Turabian Style

Bustin, Stephen; Mueller, Reinhold; Shipley, Gregory; Nolan, Tania. 2021. "COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies" Int. J. Mol. Sci. 22, no. 5: 2459. https://doi.org/10.3390/ijms22052459

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