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Article

Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications

1
Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel
2
The Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv 6997801, Israel
3
Department of Physical Chemistry, The School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Tel Aviv 6997801, Israel
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Ron Orbach
Int. J. Mol. Sci. 2021, 22(5), 2425; https://doi.org/10.3390/ijms22052425
Received: 11 February 2021 / Revised: 24 February 2021 / Accepted: 10 March 2021 / Published: 12 March 2021
(This article belongs to the Special Issue Molecular Recognition in Biological and Bioengineered Systems)
Bone tissue engineering is a rapidly developing, minimally invasive technique for regenerating lost bone with the aid of biomaterial scaffolds that mimic the structure and function of the extracellular matrix (ECM). Recently, scaffolds made of electrospun fibers have aroused interest due to their similarity to the ECM, and high porosity. Hyaluronic acid (HA) is an abundant component of the ECM and an attractive material for use in regenerative medicine; however, its processability by electrospinning is poor, and it must be used in combination with another polymer. Here, we used electrospinning to fabricate a composite scaffold with a core/shell morphology composed of polycaprolactone (PCL) polymer and HA and incorporating a short self-assembling peptide. The peptide includes the arginine-glycine-aspartic acid (RGD) motif and supports cellular attachment based on molecular recognition. Electron microscopy imaging demonstrated that the fibrous network of the scaffold resembles the ECM structure. In vitro biocompatibility assays revealed that MC3T3-E1 preosteoblasts adhered well to the scaffold and proliferated, with significant osteogenic differentiation and calcium mineralization. Our work emphasizes the potential of this multi-component approach by which electrospinning, molecular self-assembly, and molecular recognition motifs are combined, to generate a leading candidate to serve as a scaffold for bone tissue engineering. View Full-Text
Keywords: bone tissue engineering; electrospinning; self-assembly; short peptide; hyaluronic acid; scaffolds bone tissue engineering; electrospinning; self-assembly; short peptide; hyaluronic acid; scaffolds
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MDPI and ACS Style

Rachmiel, D.; Anconina, I.; Rudnick-Glick, S.; Halperin-Sternfeld, M.; Adler-Abramovich, L.; Sitt, A. Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications. Int. J. Mol. Sci. 2021, 22, 2425. https://doi.org/10.3390/ijms22052425

AMA Style

Rachmiel D, Anconina I, Rudnick-Glick S, Halperin-Sternfeld M, Adler-Abramovich L, Sitt A. Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications. International Journal of Molecular Sciences. 2021; 22(5):2425. https://doi.org/10.3390/ijms22052425

Chicago/Turabian Style

Rachmiel, Dana, Inbar Anconina, Safra Rudnick-Glick, Michal Halperin-Sternfeld, Lihi Adler-Abramovich, and Amit Sitt. 2021. "Hyaluronic Acid and a Short Peptide Improve the Performance of a PCL Electrospun Fibrous Scaffold Designed for Bone Tissue Engineering Applications" International Journal of Molecular Sciences 22, no. 5: 2425. https://doi.org/10.3390/ijms22052425

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