Next Article in Journal
Acute Tubulointerstitial Nephritis in Clinical Oncology: A Comprehensive Review
Previous Article in Journal
Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration
Open AccessArticle

The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro

1
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel
2
The Center of Advanced Research and Education in Reproduction (CARER), Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel
3
Department of OB/GYN, Soroka Medical Center, Beer Sheva 8410501, Israel
4
Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Masashi Tanaka
Int. J. Mol. Sci. 2021, 22(5), 2325; https://doi.org/10.3390/ijms22052325
Received: 29 December 2020 / Revised: 12 February 2021 / Accepted: 21 February 2021 / Published: 26 February 2021
(This article belongs to the Section Molecular Endocrinology and Metabolism)
Spermatogenesis is a complex process, in which spermatogonial cells proliferate and differentiate in the seminiferous tubules of the testis to generate sperm. This process is under the regulation of endocrine and testicular paracrine/autocrine factors. In the present study, we demonstrated that colony stimulating factor-1 (CSF-1) is produced by mouse testicular macrophages, Leydig, Sertoli, peritubular cells and spermatogonial cells (such as CDH1-positively stained cells; a marker of spermatogonial cells). In addition, we demonstrated the presence of CSF-1 and its receptor (CSF-1R) in testicular macrophages, Leydig, Sertoli, peritubular cells and spermatogonial cells of human testis. We also show that the protein levels of CSF-1 were the highest in testis of 1-week-old mice and significantly decreased with age (2–12-week-old). However, the transcriptome levels of CSF-1 significantly increased in 2–3-week-old compared to 1-week-old, and thereafter significantly decreased with age. On the other hand, the transcriptome levels of CSF-1R was significantly higher in mouse testicular tissue of all examined ages (2–12-week-old) compared to 1-week-old. Our results demonstrate the involvement of CSF-1 in the induction the proliferation and differentiation of spermatogonial cells to meiotic and postmeiotic stages (BOULE- and ACROSIN-positive cells) under in vitro culture conditions, using methylcellulose culture system (MCS). Thus, it is possible to suggest that CSF-1 system, as a testicular paracrine/autocrine system, is involved in the development of different stages of spermatogenesis and may be used in the development of future therapeutic strategies for treatment of male infertility. View Full-Text
Keywords: testis; spermatogenesis; in vitro culture of spermatogonial cells; testicular autocrine/paracrine factors; Colony stimulating factor-1 (CSF-1) testis; spermatogenesis; in vitro culture of spermatogonial cells; testicular autocrine/paracrine factors; Colony stimulating factor-1 (CSF-1)
Show Figures

Figure 1

MDPI and ACS Style

Sawaied, A.; Arazi, E.; AbuElhija, A.; Lunenfeld, E.; Huleihel, M. The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro. Int. J. Mol. Sci. 2021, 22, 2325. https://doi.org/10.3390/ijms22052325

AMA Style

Sawaied A, Arazi E, AbuElhija A, Lunenfeld E, Huleihel M. The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro. International Journal of Molecular Sciences. 2021; 22(5):2325. https://doi.org/10.3390/ijms22052325

Chicago/Turabian Style

Sawaied, Alaa; Arazi, Eden; AbuElhija, Ahmad; Lunenfeld, Eitan; Huleihel, Mahmoud. 2021. "The Presence of Colony-Stimulating Factor-1 and Its Receptor in Different Cells of the Testis; It Involved in the Development of Spermatogenesis In Vitro" Int. J. Mol. Sci. 22, no. 5: 2325. https://doi.org/10.3390/ijms22052325

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop