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Article

Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation

1
Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Vídeňská 1083, CZ 14220 Praha 4, Czech Republic
2
Department of Physiology, Faculty of Science, Charles University, Viničná 7, CZ 12844 Praha 2, Czech Republic
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Faculty of Biomedical Engineering, Czech Technical University in Prague, CZ 27201 Kladno, Czech Republic
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Laboratory of Biotransformation, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ 14220 Praha 4, Czech Republic
5
Department of Biochemistry, University of Chemistry and Technology Prague, Technická 6, CZ 16628 Praha 6, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editor: Claudiu T. Supuran
Int. J. Mol. Sci. 2021, 22(4), 1843; https://doi.org/10.3390/ijms22041843
Received: 19 January 2021 / Revised: 9 February 2021 / Accepted: 10 February 2021 / Published: 12 February 2021
(This article belongs to the Collection 21st Anniversary of IJMS: Advances in Biochemistry)
Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A165 on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A165 have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering. View Full-Text
Keywords: heterologous expression; recombinant vascular endothelial growth factor (VEGF); basic fibroblast growth factor (bFGF); adult stem cells; endothelial cells; cell adhesion; cell proliferation; tissue engineering; regenerative medicine; vascular replacements heterologous expression; recombinant vascular endothelial growth factor (VEGF); basic fibroblast growth factor (bFGF); adult stem cells; endothelial cells; cell adhesion; cell proliferation; tissue engineering; regenerative medicine; vascular replacements
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MDPI and ACS Style

Sedlář, A.; Trávníčková, M.; Matějka, R.; Pražák, Š.; Mészáros, Z.; Bojarová, P.; Bačáková, L.; Křen, V.; Slámová, K. Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation. Int. J. Mol. Sci. 2021, 22, 1843. https://doi.org/10.3390/ijms22041843

AMA Style

Sedlář A, Trávníčková M, Matějka R, Pražák Š, Mészáros Z, Bojarová P, Bačáková L, Křen V, Slámová K. Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation. International Journal of Molecular Sciences. 2021; 22(4):1843. https://doi.org/10.3390/ijms22041843

Chicago/Turabian Style

Sedlář, Antonín, Martina Trávníčková, Roman Matějka, Šimon Pražák, Zuzana Mészáros, Pavla Bojarová, Lucie Bačáková, Vladimír Křen, and Kristýna Slámová. 2021. "Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation" International Journal of Molecular Sciences 22, no. 4: 1843. https://doi.org/10.3390/ijms22041843

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