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Article

Synthesis and Biological Evaluation of Oleanolic Acid Derivatives as Selective Vascular Endothelial Growth Factor Promoter i-Motif Ligands

by
Huang Zeng
1,†,
Shuangshuang Kang
1,†,
Yu Zhang
1,
Ke Liu
1,
Qian Yu
1,
Ding Li
1,* and
Lin-Kun An
1,2,*
1
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
2
Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(4), 1711; https://doi.org/10.3390/ijms22041711
Submission received: 30 December 2020 / Revised: 4 February 2021 / Accepted: 4 February 2021 / Published: 8 February 2021
(This article belongs to the Section Biochemistry)
Browse Figures
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Abstract

:
Vascular endothelial growth factor (VEGF) is an angiogenic growth factor and plays a key role in tumor progression. The C-rich DNA sequence of VEGF promoter can form i-motif structure, which is a potential target for the development of novel anticancer agents. However, there is a limited number of chemotypes as the selective ligands of VEGF promoter i-motif, which leaves much room for development. Herein, we report the discovery of the natural oleanolic acid scaffold as a novel chemotype for the development of selective ligands of VEGF i-motif. A series of oleanolic acid derivatives as VEGF promoter i-motif ligands were synthesized. Subsequent evaluations showed that 3c could selectively bind to and stabilize VEGF promoter i-motif without significant binding to G-quadruplex, duplex DNA, and other oncogene i-motifs. Cell-based assays indicated that 3c could effectively downregulate VEGF gene transcription and expression in MCF-7 cells, inhibit tumor cells proliferation and migration, and induce cancer cells apoptosis. This work provides evidence of VEGF promoter i-motif as an anticancer target and will facilitate future efforts for the discovery of oleanolic acid-based selective ligands of VEGF promoter i-motif.

Graphical Abstract

1. Introduction

Vascular endothelial growth factor (VEGF) is an angiogenic growth factor, which plays a key role in the process of new blood vessel formation through sprouting or splitting from pre-existing vessels, resulting in stimulation of proliferation, migration, survival, and permeability of endothelial cells [1,2,3]. VEGF is commonly overexpressed in a variety of cancer cells and associated with tumor progress and viability rate. VEGF receptor and VEGF proteins have been targeted by small molecule inhibitors and monoclonal antibodies for clinically beneficial effect against a variety of tumors [4,5,6]. However, none of these strategies could affect VEGF overexpression. Therefore, development of strategy for downregulating VEGF overexpression in tumor cells could possibly provide a new approach for anti-cancer therapy.
I-motif structure consists of two parallel duplexes combined in an antiparallel manner via intercalating hemiprotonated cytosine+–cytosine base pairs (C+–C). It has been previously suggested that i-motif structure can be formed only under acidic condition in vitro. However, recent studies have shown that i-motif can exist under neutral or physiological pH under molecular crowding conditions or in the presence of some cations [7,8]. I-motif structure might play important regulatory roles in biological processes, such as DNA replication, gene transcription, and maintenance of genomic stability [9]. Some i-motif structures in oncogene promoters have been indicated for regulating gene transcriptions, including PDGFR-β, c-myc, BCL2, and KRAS [10,11,12,13,14]. It has been shown that small molecules IMC-48 and PBP1 (Figure 1) interacted with BCL2 gene promoter i-motif resulting in up-regulation of gene transcription [12,15], while B19 and a9 (Figure 1) interacted with c-myc gene promoter i-motif causing downregulation of gene transcription [14,16]. These findings suggested that i-motif structure could be targeted for regulating gene transcription.
The proximal promoter region of VEGF gene has guanine-rich and cytosine-rich strands (−85 to −50 bp relative to the initiation of transcription), which contains at least three Sp1 binding sites and controls about 90% VEGF expression. The polyG/polyC strands on this tract are very dynamic and capable of forming non-B-DNA conformations, characterized as G-quadruplexes and i-motifs, respectively [17]. Several small molecule ligands targeting four-stranded G-quadruplex structures have already been evaluated for their therapeutic potential [18,19,20]. However, the selective binder to VEGF promoter i-motif has not been developed so far.
It has been shown that steroids IMC-48 and IMC-76 could selectively bind to i-motif structure without significant interaction to G-quadruplex [12]. It is possible that the selectivity of these polycyclic structures was due to their lack of polyaromatic planar chromophore leading to decreased π–π stacking interaction to G-quartet [15,21,22]. However, their binding affinity and stability to i-motif structure is not significant, which requires further improvement [21,23,24,25]. Natural products and their derivatives are an important source for the discovery of lead compounds in drug discovery and development [26]. In our initial screening from our compound libraries, we found that oleanolic acid derivatives OA-His [27] (Figure S1A) could bind to and stabilize VEGF gene promoter i-motif (Figure S1B–D). However, the binding affinity of OA-His to VEGF promoter i-motif was still not strong enough (KD = 13.3 μM) and the stabilization effect was not significant enough (ΔTm = 6.2 ℃), which prompted us to synthesize the triterpene oleanolic acid derivatives.
The basic conformational characteristics of i-motif contain cytosine+-cytosine base pairs, loops, grooves and phosphate backbone. The lack of four-stranded planar guanine quartets in i-motif is one of the evidential features distinguished from G-quadruplexes. Based on these characteristics, we hypothesized that the ligand without large planar aromatic scaffold could have selective interaction with i-motif structure versus G-quadruplex structure. Herein, we designed and synthesized three types of oleanolic acid derivatives. To evaluate the effect of the side chains at C-28 on the binding affinity to VEGF i-motif structure, Type I compounds were synthesized as shown in Scheme 1 (3a–3q) and Scheme 2 (4a–4o). To evaluate the effect of A-ring on the binding affinity, the opened A-ring Type II compounds with a side chain were also synthesized as shown in Scheme S1 (8a–8h). To evaluate the effect of the side chain on A-ring on the binding affinity, type III compounds with various amide side chains at C-2 position were synthesized as shown in Scheme S2 (10a–10d). The synthesized compounds were initially screened by using surface plasmon resonance (SPR) assay. The active compounds with the KD value less than 50 μM were further determined by thiazole orange (TO) displacement assay and circular dichroism (CD) melting assay. The further experiments in vitro and in cells suggested that compound 3c could selectively bind to VEGF promoter i-motif, resulting in downregulation of VEGF gene transcription and proliferation inhibition of tumor cells.

2. Results

2.1. Chemistry

The type I derivatives were synthesized as shown in Scheme 1 and Scheme 2. Compounds 3a–3q were synthesized via condensation reaction of oleanolic acid (1) with diverse amine side chains following previously reported procedure (Scheme 1) [28]. The alkyne 3a could further react with various azide materials through copper (I) catalyzed click chemistry to give the target compounds 4a–4p (Scheme 2) [29].
As shown in Scheme S1, oleanolic acid reacted with CH3I to give the methyl oleanolate 5, which was oxidized with Jones reagent to give the ketone intermediate 6 [30]. Treatment of compound 6 with m-chloroperoxybenzoic acid produced 3,4-lactone-12,13-epoxy intermediate 7. The stereochemistry of this epoxy group was determined through a NOE correlation between H-12 (δ 3.17) and H-18 (δ 2.00), suggesting an α-configuration. The reaction of intermediate 7 with amines mainly gave the type II compounds 8a–8h. The NOE spectrum revealed a correlation between the signals of H-13 (δ 2.66) and H-26 (δ 0.96), suggesting a β-configuration for H-13, which have more stable trans junction between rings C and D [31,32].
As shown in Scheme S2, intermediate 6 could be reacted with Stiles’ reagent and then treated with DDQ to yield the acid 9 [30]. The type III compounds 10a–10d were prepared via condensation reaction of 9 with amines.
Finally, 44 oleanolic acid derivatives were synthesized and 40 of them were new compounds (3c–3q, 4a–4m, 8a–8h, 10a–10d were newly synthesized; 3a [29], 3b [33], 4n [29], 4o [29] have been previously reported). The structures of the new derivatives were characterized through NMR and HRMS spectra. The purity of all derivatives for biological evaluation was assessed through evaporative light scattering detector (ELSD)/high performance liquid chromatography (HPLC) method and determined to be more than 95%.

2.2. Initial Screening

SPR experiments were carried out to investigate the binding affinities of the synthesized compounds to VEGF promoter i-motif, VEGF promoter G-quadruplex and the duplex DNA. The oligomer Py24 and Pu24 were used as the VEGF i-motif and G-quadruplex forming sequence respectively (Table S1). As shown in Table 1, thirteen active compounds were found to have binding affinity to VEGF i-motif with KD values ranging from 1.95 to 31.3 μM, and all tested compounds had no obvious binding affinity to VEGF G-quadruplex structure and duplex DNA. The compounds 3c, 3h, and 3i with a long and flexible polyamine chain at C-28 position had good binding affinity to VEGF i-motif with the KD values of 1.95 (Figure 2A), 3.09 and 5.01 μM, respectively. In comparison, compounds 3j–3q with relatively rigid side chain did not show binding affinity. In addition, it seems that the hydroxyl terminal functional group of the side chain at C-28 also showed important role on the binding affinity to VEGF i-motif structure. For example, compounds 3b and 4o had KD values of 11.4 and 12.0 μM, respectively. Besides, most type II compounds with A-ring opened and modified derivatives had no obvious binding affinity to VEGF i-motif. Only two A-ring opened derivatives, 8b and 8c, showed moderate interaction with KD values of 31.3 and 21.1 μM, respectively; 10a–10d did not show obvious interaction to VEGF i-motif.
To confirm their binding affinity, the thirteen active compounds 3b–3e, 3h, 3i, 4a, 4b, 4k, 4m, 4o, 8b, and 8c were selected and further assessed through fluorescent intercalator displacement (FID) assays using thiazole orange. Relative TO displacement ratio (%) was determined at a fixed Py24 concentration (1 μM) with or without the tested compound at a fixed concentration (5 μM). As shown in Table 1, the displacement ratios were basically consistent with the KD values found in SPR assays. Among them, 3c and 3i have significant displacement ratios of 67% and 51%, respectively.
An ideal i-motif ligand should possess two essential features: excellent i-motif binding affinity and excellent i-motif stabilizing ability. In order to investigate the thermodynamic stabilization effect of these thirteen active derivatives on VEGF i-motif, CD melting experiment was also performed. As shown in Figure 2B and Figure S2, with the addition of five equivalents 3c, the melting temperature (Tm) of Py24 enhanced up to 19.1 ℃, indicating that 3c had significant stabilizing ability to VEGF promoter i-motif. Besides, the compound 3i had moderate ΔTm values (7.0 °C, Table 1). It has been reported that the polyamines could stabilize DNA secondary structures [34,35]. The significantly higher ΔTm values of 3c than the other derivatives promoted us to investigate whether the polyamine side chain (1,5,8,12-tetraazadodecane) of 3c could stabilize Py24 individually. The results showed that no significant change of Tm value of Py24 was observed with the addition of 1,5,8,12-tetraazadodecane (Figure S3), which indicated that the thermal stability effect of 3c on Py24 was dependent on the properties of overall molecule structure. Further CD melting experiments indicated that 3c had weak effect on VEGF G-quadruplex and other promoter i-motif structures (BCL2, KRAS, c-kit, PDGFR-β) with ΔTm values ranging from 0 to 2.6 ℃ (Figure S4), indicating 3c could selectively stabilize VEGF i-motif structure.
Based on these results, compound 3c was selected for the subsequent biological experiments.

2.3. 3c Could Selectively Bind to VEGF i-Motif Structure

To confirm the interaction of 3c with VEGF i-motif, the microscale thermophoresis (MST) experiment was conducted. After incubation of 3c with 5′-FAM-labeled VEGF promoter C-rich oligomers (FPy24), MST analysis was performed and KD value was determined to be 1.14 μM (Figure 2C), which was consistent with the KD value of 1.93 μM determined by using SPR assay. The interactions of 3c to VEGF G-quadruplex, Duplex DNA and other oncogene promoter i-motif structures, such as BCL2, KRAS, c-kit, PDGFR-β, were also determined by using MST. As shown in Figure 2D and Figure S5, the interactions of 3c to the G-quadruplex, duplex DNA and other oncogene promoter i-motifs were relatively weaker with the KD values ranging from 14.4 to 95.1 μM.
The binding affinity and selectivity of 3c was further investigated by using TO displacement and the concentrations of 3c required to displace TO by 50% (DC50) from DNA secondary structures were determined. As shown in Figure 2E,F, 3c could effectively displace the bound TO from pre-folded Py24 with DC50 of 3.19 μM. In comparison, 3c exhibited lower affinity for Pu24, BCL2, KRAS, c-kit, and PDGFR-β with DC50 ranging from 8.01 to 16.1 μM. The results of MST and TO experiments suggested that 3c had binding selectivity to VEGF promoter i-motif.
The interaction mode was investigated by using ESI-MS experiment. The ESI-MS spectra showed that the peak at m/z 7794.2 corresponding to the mass weight of Py24-3c adduct was observed after annealing VEGF promoter i-motif forming sequence Py24 with 3c at pH 5.0 (Figure 3A), indicating that 3c could bind to VEGF promoter i-motif with stoichiometry of 1:1. Conversely, the peak for Py24-3c adduct was not observed under neutral annealing condition (Figure S6). The interaction mode of 3c with VEGF promoter i-motif was further studied by using isothermal titration calorimetry (ITC) experiment. The negative value of ΔH indicated that 3c interacted with VEGF i-motif possibly via van der Waals interaction and hydrogen bond. The negative value of Gibbs free energy (ΔG) indicated that their binding was a spontaneous process. The binding enthalpy was well fitted with a 1:1 binding mode, and the KD value was calculated to be 1.13 μM (Figure 3B).

2.4. 3c Could Induce the Formation of VEGF i-Motif

To evaluate the ability of 3c to induce the formation of VEGF promoter i-motif, CD titration experiment was conducted. The CD spectra of the oligomer Py24 showed a positive peak at 288 nm and a negative peak at 265 nm under acidic condition (pH ≤ 5.5), indicating the formation of i-motif structure (Figure S7). Under the conditions of pH > 6.0, the positive peak at 288 nm decreased significantly, indicating disruption of i-motif structure. Upon the addition of 3c at pH 6.0, the CD spectra exhibited a dose-dependent enhancement of the positive peak (~288 nm) along with a red shift from 276 nm to 288 nm, implying that 3c could induce the formation of VEGF promoter i-motif (Figure 4A). Conversely, 3c could not induce the formations of VEGF G-quadruplex and other promoter i-motifs, including BCL2, KRAS, c-kit, PDGFR-β (Figure S8).
Fluorescence resonance energy transfer (FRET)–quenching experiment was also conducted to confirm the induction of 3c to the formation of VEGF i-motif. The VEGF gene promoter i-motif forming sequence containing a FAM (6-carboxy-fluorescein) label at 5′ and a TAMRA (5-carboxytetramethylrhodamine) label at 3′ (FPy24T, Table S1) was used. As shown in Figure 4B, the fluorescence quenching of FPy24T solution was obviously found with the titration of 3c, implying that 3c could induce the formation of VEGF i-motif structure.
1H NMR titration experiment was also performed to study the effect of 3c on the secondary structure transformation of oligomer Py24. As shown in Figure 4C, at pH 6.0 condition, species corresponding to a duplex/hairpin and an i-motif were both observed in 1H NMR spectra of Py24. The imino protons at 15–16 ppm are characteristic of hemiprotonated C+–C base pairs in an i-motif, while the imino peaks at 13 ppm are characteristic of Watson–Crick base pairs in a duplex or hairpin conformation [12]. In comparison, at pH 5.0 condition, signal peaks for i-motif species increased while signal peaks for hairpin/duplex species decreased, implying a shift of the equilibrium from hairpin/duplex to i-motif structure, which was consistent with the CD assay results (Figure S7). Upon addition of 3c, the equilibrium shifts from hairpin/duplex to i-motif structure were observed in a dose-dependent manner (Figure 4C). With the addition of 10 equivalents of 3c, no signal (∼13 ppm) for hairpin/duplex species was observed.
The above results strongly supported that 3c could significantly induce the formation of VEGF promoter i-motif structure.

2.5. 3c Could Downregulate VEGF Gene Transcription and Protein Expression

To study the effect of 3c on the gene transcription in cells, dual-luciferase reporter assay was performed. We cloned the wide-type (Wt-VEGF), devoid (Del-VEGF), and mutant (Mut-VEGF) sequences of VEGF corresponding upstream i-motif elements into pGL 4.10 vector respectively (Figure 5A and Figure S9), and transiently transfected them into MCF-7 cells followed by the treatment with 3c for 24 h. As shown in Figure 5B, the luciferase activity was found decreased in a dose-dependent manner for the plasmid containing wild-type VEGF promoter i-motif forming sequence. In contrast, no significant decrease of luciferase activity was observed for the plasmid containing devoid and mutated VEGF promoter i-motif forming sequence.
Quantitative real time PCR assay was also conducted to analyze the transcription levels. As shown in Figure 5C, after treatment of MCF-7 cells with 3c for 48 h, VEGF mRNA level was found to be reduced significantly in a dose-dependent manner. In contrast, 3c had no significant effect on the transcription of other oncogenes, including BCL2, KRAS, c-kit, and PDGFR-β.
The effect of 3c on VEGF protein expression was determined by using Western blot experiment. As shown in Figure 5D, 3c could reduce VEGF protein expression in a dose-dependent manner in MCF-7 cells. In contrast, 3c had no significant effect on expression of other oncogene proteins, including PDGFR-β, c-kit, and BCL-2 (Figure S10).
These results indicated that 3c could selectively interact with VEGF promoter i-motif, resulting in downregulation of VEGF gene transcription and protein expression in cancer cells.

2.6. 3c Could Induce Apoptosis and Inhibit Proliferation and Metastasis of Cancer Cells

Annexin–fluorescein isothiocyante (FITC)/propidine iodide (PI) double-staining flow cytometry was carried out to analyze cell apoptosis induced by 3c (Figure 6A). After being treated with 3c, the percentages of both early and late apoptotic MCF-7 cells significantly increased. Total 39.6% of apoptotic cells were found after being treated with 6 μM of 3c.
Since 3c could downregulate VEGF gene transcription and expression in cancer cells, the effects of 3c on the proliferation and migration of cancer cells were determined. The methyl thiazolyl tetrazolium (MTT) colorimetric assay indicated that 3c exhibited dose-dependent anti-proliferation activities in MCF-7 cells with its IC50 values (the concentration for 50% inhibition) determined to be 6.5 μM, as shown in Supplementary Figure S11. The colony formation assay further demonstrated that the ability of MCF-7 cells to form colonies was significantly decreased in a dose-dependent manner upon treatment with increasing concentration of 3c (Figure 6B). In addition, the scrape assay indicated that MCF-7 cell metastasis was slowed down in a dose-dependent manner after being treated with 3c for 48 h and 96 h period (Figure 6C).
These results indicated that 3c could inhibit the proliferation and migration of cancer cells and induce cancer cells apoptosis possibly through downregulating VEGF gene transcription and translation.

2.7. Molecular Modeling

Molecular docking studies were performed to understand possible interactions of 3c with VEGF promoter i-motif structure using the MOE program. Because neither the NMR nor the X-ray crystallographic structure of VEGF promoter i-motif has been reported yet, the telomeric i-motif structure (PDB ID:1ELN) [36] was used as a model structure for analyzing possible binding interaction of 3c with i-motif. As shown in Figure 7, the docking results revealed that the pentacyclic triterpenoid backbone of 3c possibly lay on the i-motif groove space. The polyamine side chain of 3c might be positively charged in physiological conditions, and form electrostatic interactions toward the negatively charged phosphate diester backbone of DNA. The terminal amino side chain and the hydroxyl group of 3c could form two important H-bonds with phosphate backbone with the distances of 2.3 and 3.1 Å, which facilitated 3c twining around the i-motif structure. This could help to rationalize the significant thermal stability effect of 3c on Py24. Furthermore, in order to compare the binding mode of 3c with i-motif and G-quadruplex, the molecular docking of 3c with VEGF promoter G-quadruplex (PDB ID: 2M27) [37] was also performed. The results showed that pentacyclic triterpenoid backbone of 3c was unable to form the π–π stacking interaction with the planar of G-quadruplex (Figure S12). With the exception of an H-bond forming between the polyamine side chain and dT22 site of phosphate diester backbone, 3c couldn’t form effective interaction with G-quadruplex. These results explained the reason of the selective interaction of 3c with i-motif versus G-quadruplex.

3. Conclusions

In summary, a series of oleanolic acid derivatives as VEGF promoter i-motif ligands were synthesized. The SPR experiment, TO displacement experiment and CD melting experiment were carried out to study their binding affinity and stabilizing ability to VEGF i-motif promoter. Among them, 3c was identified as a promising ligand, which could selectively bind to and stabilize VEGF promoter i-motif structure, without significant interaction to VEGF G-quadruplex, duplex DNA and other oncogene promoter i-motifs, including BCL2, c-kit, KRAS, and PDGFR-β i-motifs. Cell-based assays indicated that 3c could effectively downregulate VEGF gene transcription and translation, and inhibit tumor cells proliferation and migration, and promote cancer cells apoptosis. Therefore, 3c could be used as a tool compound for the investigation on the role of VEGF promoter i-motif in regulating oncogene transcription, and as a promising lead compound for further development for anticancer therapy. This work proved that the natural oleanolic acid scaffold represents a chemotype for selective ligands of VEGF i-motif and offered a new anticancer strategy with VEGF promoter i-motif as a new target.

4. Materials and Methods

4.1. General Materials

All chemicals and starting materials were purchased from commercial sources, which were analytical grade without further purification unless otherwise specified. NMR spectra were recorded on a Bruker BioSpin GmbH 400 MHz or 500 MHz spectrometer using TMS (tetramethylsilane) as the internal standard. High-resolution mass spectra (HRMS) were recorded on Shimadzu LCMS-IT-TOF of MAT95XP mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). All oligomers/primers used in this study were purchased from Invitrogen or Sangon, as shown in Table S1 in the Supporting Information. CD experiments were performed on a Chirascan circular dichroism spectrophotometer (Applied Photophysics, Leatherhead, UK). Fluorescence experiments were performed on Fluoromax-4 luminescence spectrophotometer (Horiba Jobin-Yvon, Paris, France). ITC experiments were performed using a MicroCal VP-ITC instrument (GE Healthcare, Northampton, MA, USA). For obtaining i-motif structures, C-rich oligonucleotides were annealed in 1× BPES buffer (30 mM KH2PO4, 30 mM K2HPO4, 1 mM EDTA, 100 mM KCl) with different pH at 95 °C for 5 min, and then cooled to room temperature. For obtaining G-quadruplex, oligonucleotides were annealed in 20 mM Tris–HCl buffer containing 100 mM KCl (pH 7.4) by heating at 95 °C for 5 min followed with gradual cooling to room temperature.

4.2. Synthesis

All synthetic procedures, yields, and physical and spectroscopic data for the compounds are included in the Supplementary Materials.

4.3. Surface Plasmon Resonance (SPR) Assay

SPR assay was performed on a ProteOn XPR36 Protein Interaction Array system (Bio-Rad Laboratories, Hercules, CA, USA) using a NeutrAvidin-coated GLH sensor chip. For immobilization, all DNA samples were biotinylated and attached to a reptavidin-coated sensor chip. 5′-Biotin labeled Py24 were diluted to 1 μM in running buffer (20 mM 2-(4-morpholino)ethanesulfonic acid, pH 5.5, 100 mM KCl and 0.05% Tween-20), and 5′-biotin Pu24 and 5′-biotin duplex DNA were diluted to 1 μM in running buffer (Tris-HCl 20 mM, pH 7.4, 100 mM KCl). The DNA samples were then captured (1000 RU) in flow cells, and a blank cell was set as a control. All ligands were prepared with the running buffer through serial dilutions from stock solution (10 mM in DMSO). Ligands of different concentrations were injected at a flow rate of 30 μL/min for 120 s of association phase, followed with 120 s of dissociation phase at 25 °C. The GLH sensor chip was regenerated with short injection of 50 mM NaOH between consecutive measurements. The final graphs were obtained by subtracting blank sensorgrams from the i-motif, G-quadruplex, or duplex sensorgrams. Data was analyzed by using ProteOn manager software.

4.4. Microscale Thermophoresis (MST) Assay

The thermophoresis movements of the fluorescently labeled nucleic acids and 3c complexes were analyzed by monitoring the fluorescence distributions inside the capillary by using the NT.115 MST instrument (NanoTemper, München, Germany). The concentration of DNA was held constant at 1 μM. The compound was diluted at 1:1 from 100 μM for 16 times. The samples were loaded into standard-treated MST-grade glass capillaries. The intensities of the light-emitting diode (LED) and laser were set as 40% and 40%, respectively. Data was analyzed by using NT Analysis 1.4.23 software (NanoTemper, München, Germany).

4.5. TO Displacement Assay

I-motif or G-quadruplex oligonucleotides solution (1 μM, 100 μL) was added to the cuvette, followed with addition of thiazole orange (2 μM). The spectrum of the oligonucleotide with titration of 3c was recorded based on fluorescence emission (λex = 480 nm).

4.6. ESI-MS Assay

The oligomer Py24 (5 µM) was annealed in the presence or absence of 3c in 1 × BPES buffer (10 mM KH2PO4, 10 mM K2HPO4, 1 mM EDTA, 100 mM KCl, pH 5.0 or pH 7.5) at 95 °C for 5 min. The solution was gradually cooled to room temperature, and detected by using an electrospray ionization (ESI) mass spectrometer.

4.7. Isothermal Titration Calorimetry Assay

The oligomer Py24 was diluted from stock to the indicated concentration (0.80 mM) in 1 × BPES buffer at pH 5.0 and then annealed by heating at 95 °C for 5 min. The solution was gradually cooled to room temperature to generate i-motif structure. The oligomer Py24 was titrated into 3c (0.08 mM) at 30 °C in 10 μL injections with a spacing of 300 s between injections. The integrated heat data were corrected for the heat of dilution and blank effects. The binding isotherms were fitted with the one-site binding model incorporated into the MicroCal Origin VPITC software.

4.8. Circular Dichroism Spectroscopy

A quartz cuvette with 4 mm path length was used for the spectra recorded over a wavelength range of 230–350 nm at 1 nm bandwidth. The oligomer Py24 was diluted from stock to the required concentration (1 μM) in 1 × BPES buffer at pH 5.0 or 6.0 and then annealed by heating at 95 °C for 5 min. The solution was gradually cooled to room temperature, and incubated with 3c at 37 ℃ for 1 h. Spectra were recorded three times, averaged, smoothed, and baseline corrected to remove signal contribution from buffer. The data was analyzed by using Origin 9.0 (OriginLab, Northampton, MA, USA).
For CD melting assays, thermal melting was monitored at 230–350 nm with a 1 nm bandwidth. CD melting assays were performed at a fixed DNA concentration (2 μM) with or without 3c in relevant buffer. The data were recorded at intervals of 5 °C over a range of 20–95 °C with a heating rate of 1 °C/min.

4.9. Fluorescence Resonance Energy Transfer (FRET) Assay

Oligomer Py24 with a 5′-end FAM-fluorophore and a 3′-end TAMRA are provided in Table S1. The fluorescence of a fixed concentration (0.1 μM) of oligonucleotide was tested (λex = 480 nm) with the titration of 3c.

4.10. NMR Titration Experiment

The DNA oligonucleotide was purchased from Sangon (China). The final NMR samples were prepared in 1×BPES buffer containing 10% D2O at pH 5.0 or 6.0. The concentration of DNA sample was set at 1.0 mM. The stock solution of 3c was dissolved in DMSO-d6. 1H NMR titration experiments were performed on a Bruker DRX-600 MHz spectrometer at 25 °C, and the water signal was suppressed.

4.11. Cell Culture

Human breast adenocarcinoma cell line MCF-7 was purchased from China Center for Type Culture Collection in Wuhan. The cell line was maintained in DMEM (Dulbecco’s Modified Eagle’s medium), supplemented with 10% fetal calf serum, 100 U/mL Penicillium, and 100 mg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO2.

4.12. MTT Assay

Human breast adenocarcinoma cells MCF-7 were seeded on 96-well plates (5000 cells/well) and treated with 3c at various concentrations. After 48 h treatment at 37 °C in a humidified atmosphere of 5% CO2, 20 μL of 2.5 mg/mL 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) solution was added to each well and further incubated for 4h. The cells in each well were then treated with DMSO (200 μL for each well), and the optical density (OD) was recorded at 570 nm. The cytotoxicity was evaluated based on the percentage of cell survival in a dose-dependent manner regard to the blank. The final IC50 values (the concentration for 50% inhibition) were calculated by using the Prism 8 Computer Software (GraphPad, San Diego, CA, USA). All of the experiments were repeated three times.

4.13. Colony Formation Assay

MCF-7 cells were subsequently seeded in 6-well culture plates (1000/well) for 24 h pre-culture at 37 °C in a humidified atmosphere with 5% CO2, and then treated with 3c at different concentrations for 9 days. The cells were washed with 1 × PBS and fixed with ice cold methanol for 10 min, followed by the addition of 0.5% crystal violet solution for 30 min to observe the colony formation.

4.14. Cell Scrape Assay

MCF-7 cells were subsequently seeded in 6-well culture plates (300,000/well) at 37 °C in a humidified atmosphere with 5% CO2. After being pre-cultured for 48 h, a cross-shaped scrape was made through the monolayer Siha cells using a plastic pipet tip, and then the cells were treated with 3c at different concentrations. The wounded areas were observed and photographed using microscopy after being incubated for the indicated times.

4.15. Flow Cytometric Analysis

MCF-7 cells were seeded in 6-well plate (2 × 105/well) and incubated for 24 h at 37 °C in a humidified atmosphere with 5% CO2. After being incubated in the presence and absence of 3c for 48 h, the Siha cells were then washed in PBS, and centrifuged and re-suspended in Annexin V–FITC solution for 15 min at room temperature in dark. After centrifuged for 5 min, the cells were then re-suspended in Annexin V–FITC solution and mixed with PI staining solution for 10 min at 2–5 °C in dark. Then, the cells were analyzed by using flow cytometry with an Epics Elite flow cytometer (Beckman Coulter, Brea, CA, USA).

4.16. Dual-Luciferase Reporter Assay

A total of 100 ng of constructed pGL4.10 luciferase plasmid (Promega, Madison, WI, USA) containing VEGF corresponding upstream i-motif elements and 100 ng plt-tk Renilla plasmid were transfected into MCF-7 cells by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). After 6 h, the solution of 3c was added to the cells. The cells were incubated at 37 °C with CO2 for 24 h, and the transfected cells were first washed with ice-cold PBS to reduce the background signals from the medium. Luciferase assays were subsequently performed according to the manufacturer’s instructions using the dual-luciferase assay system (Promega, Madison, WI, USA). After a 3 s delay, secreted luciferase signals were collected for 10 s using a microplate reader (Molecular Devices, Sunnyvale, CA, USA). The quantification was performed using a multimode reader (Molecular Devices, Sunnyvale, CA, USA). The secreted Renilla luciferase activity was normalized to the firefly luciferase activity.

4.17. RNA Extraction and Real Time Polymerase Chain Reaction (RT-PCR)

MCF-7 cells were seeded in 6-well plate (2 × 105 cells/well), and incubated for 24 h at 37 °C in a humidified atmosphere with 5% CO2. After the cells were incubated in the presence or absence of 3c for 48 h, cells were harvested, and the RNA was extracted by using HiPure Total RNA Mini Kit (Magen, Guangzhou, China). Quantitative real-time polymerase chain reaction (PCR) was carried out using 2×RealStar SYBR Mixture (Genstar, Guangzhou, China). The results were analyzed on a LightCycler480 II real-time PCR system (Roche, Basel, Switzerland). The oncogene mRNA levels were normalized to β-actin mRNA level of each sample. Results of real-time PCR were analyzed by using the 2−ΔCt method.

4.18. Western Blot Assay

MCF-7 cells were seeded in 6-well plate (2 × 105 cells/well) and incubated for 24 h at 37 °C in a humidified atmosphere with 5% CO2. After being incubated in the presence and absence of 3c for 48 h, cells were harvested from each well of culture plates and lysed in 200 μL of protein extraction buffer consisting of 1 mM PMSF for 30 min. The suspension was centrifuged at 10,000 rpm at 4 °C for 15 min, and the protein content of supernatant was measured by using bicinchoninic acid (BCA) assay. The same amount of protein for each sample was loaded onto 8% polyacrylamide gel, and then transferred to a microporous polyvinylidene difluoride (PVDF) membrane. Western blotting was performed by using anti-VEGF and anti-β-GAPDH (cell signaling technology) antibodies, as well as horseradish peroxidase-conjugated anti-rabbit secondary antibody. Protein bands were visualized by using chemiluminescence substrate.

4.19. Molecular Docking

The telomeric i-motif DNA and VEGF G-quadruplex structures were downloaded from Protein Data Bank (1ELN, 2M27). After optimization of the ligand and assigning partial atomic charges, docking calculations were performed with the MOE program. The ligand to be modeled was constructed and optimized by using ChemDraw and saved in SDF file format, and was minimized with the conjugate gradient method using the MMFF94x force field with MMFF94 charges. The process of site finder was performed using MOE software (Chemical Computing Group, Montreal, Canada) to determine the binding sites. Docking runs were carried out and the ligand conformation was chosen based on the docked energy. The final graphs were created by using PyMOL software (Schrödinger, New York, NY, USA).

Supplementary Materials

Supplementary data to this article can be found online at https://www.mdpi.com/1422-0067/22/4/1711/s1.

Author Contributions

Chemical synthesis, biological experiments, acquisition, analysis and interpretation of data, preparation of manuscript, docking studies (H.Z.); biological experiments, acquisition, analysis and interpretation of data, preparation of manuscript (S.K.); biological experiments, acquisition of data (Y.Z.); chemical synthesis (K.L.); chemical synthesis (Q.Y.); conception and design, analysis, and interpretation of data, revision of the manuscript (D.L.); conception and design, study supervision, analysis and interpretation of data, revision of the manuscript (L.-K.A.). All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (21977123), Guangdong Basic and Applied Basic Research Foundation (2019A1515011317, 2019A1515011074), Guangdong Provincial Key Laboratory of Construction Foundation (2017B030314030), and the Fundamental Research Funds for the Central Universities (19YKPY127).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in both article and Supplementary Materials.

Conflicts of Interest

The authors confirm that this article content has no conflict of interest.

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Ijms 22 01711 g001 550
Figure 1. Structures of reported i-motif binding ligands, including steroids IMC-48 and IMC-76, acridone B19, peptidomimetic PBP1, and bisacridine derivatives a9.
Figure 1. Structures of reported i-motif binding ligands, including steroids IMC-48 and IMC-76, acridone B19, peptidomimetic PBP1, and bisacridine derivatives a9.
Ijms 22 01711 g001
Ijms 22 01711 sch001 550
Scheme 1. Synthetic route of type I compounds 3a–3q. Reagents and conditions: (a) HBTU, DIPEA, THF, r.t., 2 h (yield 93%). (b) Amines, K2CO3, DMF, r.t., 1 h (yield 69–93%). (c) (1) N-Boc-protected amines, K2CO3, DMF, r.t., 1 h; (2) HCl/EA, for 3c (80% yield for two steps).
Scheme 1. Synthetic route of type I compounds 3a–3q. Reagents and conditions: (a) HBTU, DIPEA, THF, r.t., 2 h (yield 93%). (b) Amines, K2CO3, DMF, r.t., 1 h (yield 69–93%). (c) (1) N-Boc-protected amines, K2CO3, DMF, r.t., 1 h; (2) HCl/EA, for 3c (80% yield for two steps).
Ijms 22 01711 sch001
Ijms 22 01711 sch002 550
Scheme 2. Synthetic route of type I compounds 4a–4o. Reagents and conditions: (a) R-N3, CuSO4, Na-L-ascorbate, THF/H2O, 40 °C, 8 h (yield 70–90%). (b) NaOH, H2O, MeOH, r.t., 7 h (yield 89%).
Scheme 2. Synthetic route of type I compounds 4a–4o. Reagents and conditions: (a) R-N3, CuSO4, Na-L-ascorbate, THF/H2O, 40 °C, 8 h (yield 70–90%). (b) NaOH, H2O, MeOH, r.t., 7 h (yield 89%).
Ijms 22 01711 sch002
Ijms 22 01711 g002 550
Figure 2. (A) Sensorgrams for the binding of 3c to VEGF promoter C-rich oligomers (Py24) with KD value determined to be 1.95 μM by using SPR. (B) CD melting curves for Py24 in the absence and presence of 5 equivalents of 3c in 1× BPES buffer at pH 5.5. (C) Microscale thermophoresis (MST) analysis for interactions between 5′-FAM-labeled VEGF promoter C-rich oligomers (FPy24) and 3c. (D) The binding constants (KD) were determined by using MST for interaction of 3c with various 5′-FAM-labeled oligomers. (E) Fluorescence spectra analysis of TO displacement experiment for Py24 (1 μM) with the increasing addition of 3c. (F) The plots of displacement versus concentration of 3c for various oligomers (λex = 480 nm, λem = 530 nm), with DC50 values determined as the concentrations of the ligands required to displace TO by 50%. 3c exhibited DC50 values of 3.19 μM, 8.08 μM, 8.29 μM, 13.1 μM, 14.7 μM, and 16.1 μM for Py24, PDGFR-β, Pu24, KRAS, c-kit, and BCL2.
Figure 2. (A) Sensorgrams for the binding of 3c to VEGF promoter C-rich oligomers (Py24) with KD value determined to be 1.95 μM by using SPR. (B) CD melting curves for Py24 in the absence and presence of 5 equivalents of 3c in 1× BPES buffer at pH 5.5. (C) Microscale thermophoresis (MST) analysis for interactions between 5′-FAM-labeled VEGF promoter C-rich oligomers (FPy24) and 3c. (D) The binding constants (KD) were determined by using MST for interaction of 3c with various 5′-FAM-labeled oligomers. (E) Fluorescence spectra analysis of TO displacement experiment for Py24 (1 μM) with the increasing addition of 3c. (F) The plots of displacement versus concentration of 3c for various oligomers (λex = 480 nm, λem = 530 nm), with DC50 values determined as the concentrations of the ligands required to displace TO by 50%. 3c exhibited DC50 values of 3.19 μM, 8.08 μM, 8.29 μM, 13.1 μM, 14.7 μM, and 16.1 μM for Py24, PDGFR-β, Pu24, KRAS, c-kit, and BCL2.
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Figure 3. (A) ESI-MS spectra of VEGF promoter C-rich oligomer (Py24) without (upper) and with (bottom) addition of 3c at pH 5.0. The mass of 7794.2 Da is corresponding to py24-3c adduct. (B) Isothermal titration calorimetry (ITC) experiment for the binding interaction of Py24 with 3c. The upper panel showed heat change upon ligand titration. The bottom panel showed the integrated data and ITC isotherm (solid line) fitted by a single-site binding model.
Figure 3. (A) ESI-MS spectra of VEGF promoter C-rich oligomer (Py24) without (upper) and with (bottom) addition of 3c at pH 5.0. The mass of 7794.2 Da is corresponding to py24-3c adduct. (B) Isothermal titration calorimetry (ITC) experiment for the binding interaction of Py24 with 3c. The upper panel showed heat change upon ligand titration. The bottom panel showed the integrated data and ITC isotherm (solid line) fitted by a single-site binding model.
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Figure 4. (A) CD spectra of VEGF promoter C-rich oligomers (Py24) in the absence and presence of 3c at the indicated pH. (B) Fluorescence resonance energy transfer (FRET) titration experiments of dual labeled VEGF promoter C-rich oligomer (FPy24T) with 3c at pH 6.0. (C) 1H NMR spectra of the imino proton region of Py24 upon titration of 3c at pH 6.0.
Figure 4. (A) CD spectra of VEGF promoter C-rich oligomers (Py24) in the absence and presence of 3c at the indicated pH. (B) Fluorescence resonance energy transfer (FRET) titration experiments of dual labeled VEGF promoter C-rich oligomer (FPy24T) with 3c at pH 6.0. (C) 1H NMR spectra of the imino proton region of Py24 upon titration of 3c at pH 6.0.
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Figure 5. (A) Schematic diagram of dual-luciferase plasmids constructed with Wt-VEGF, Del-VEGF and Mut-VEGF i-motif-forming sequences of the VEGF promoter. (B) Effect of 3c on Wt-VEGF, Del-VEGF, and Mut-VEGF promoter determined by using dual luciferase reporter assays. (C) The mRNA levels of VEGF, BCL2, KRAS, c-kit, PDGFR-β in MCF-7 cells were analyzed by using qRT-PCR after incubation with increasing concentration of 3c. (D) Western blot results of VEGF protein in MCF-7 cells in the presence of increasing concentrations of 3c from 0 to 6 μM. The GAPDH protein was used as a control. The experiments were repeated for three times. The data are expressed as the mean ± SEM: (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001.
Figure 5. (A) Schematic diagram of dual-luciferase plasmids constructed with Wt-VEGF, Del-VEGF and Mut-VEGF i-motif-forming sequences of the VEGF promoter. (B) Effect of 3c on Wt-VEGF, Del-VEGF, and Mut-VEGF promoter determined by using dual luciferase reporter assays. (C) The mRNA levels of VEGF, BCL2, KRAS, c-kit, PDGFR-β in MCF-7 cells were analyzed by using qRT-PCR after incubation with increasing concentration of 3c. (D) Western blot results of VEGF protein in MCF-7 cells in the presence of increasing concentrations of 3c from 0 to 6 μM. The GAPDH protein was used as a control. The experiments were repeated for three times. The data are expressed as the mean ± SEM: (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001.
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Figure 6. (A) Flow cytometry dot plot of apoptosis in MCF-7 cells induced by 3c at 1.5, 3, and 6 μM, respectively. (B) The colony formation of MCF-7 cells after being treated with 3c (1.5, 3, and 6 μM) for 9 days. (C) The metastasis of MCF-7 cells after being treated with 3c (3 and 6 μM) for the indicated times.
Figure 6. (A) Flow cytometry dot plot of apoptosis in MCF-7 cells induced by 3c at 1.5, 3, and 6 μM, respectively. (B) The colony formation of MCF-7 cells after being treated with 3c (1.5, 3, and 6 μM) for 9 days. (C) The metastasis of MCF-7 cells after being treated with 3c (3 and 6 μM) for the indicated times.
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Figure 7. Hypothetical binding mode of 3c to i-motif secondary structures. Top views (A) and side-views (B) for the interactions of 3c with telomeric i-motif DNA (PDB ID: 1ELN) as well as the electrostatic interactions of the ligands with the phosphate backbone (shown in yellow dashed lines).
Figure 7. Hypothetical binding mode of 3c to i-motif secondary structures. Top views (A) and side-views (B) for the interactions of 3c with telomeric i-motif DNA (PDB ID: 1ELN) as well as the electrostatic interactions of the ligands with the phosphate backbone (shown in yellow dashed lines).
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Table
Table 1. Equilibrium binding constants (KD), relative thiazole orange (TO) displacement ratio (%) and stabilization temperatures (ΔTm) of the synthesized compounds.
Table 1. Equilibrium binding constants (KD), relative thiazole orange (TO) displacement ratio (%) and stabilization temperatures (ΔTm) of the synthesized compounds.
Comp.KD (μM) 1TO (%) 2ΔTm (°C) 3Comp.KD (μM)TO (%)ΔTm (°C)
Py24Pu24DuplexPy24Py24Py24Pu24DuplexPy24Py24
OA-His 13.3 >50 4 >50 n.d. 5 6.2 4g >50 >50 >50 n.d. n.d.
3b 11.4 >50 >50 27% 1.0 4h >50 >50 >50 n.d. n.d.
3c 1.95 >50 >50 67% 19.1 4i >50 >50 >50 n.d. n.d.
3d 12.1 >50 >50 13%. −1.6 4j >50 >50 >50 n.d. n.d.
3e 13.8 >50 >50 20% 0.6 4k 12.3 >50 >50 15% 3.3
3f >50 >50 >50 n.d. n.d. 4l >50 >50 >50 n.d. n.d.
3g >50 >50 >50 n.d. n.d. 4m 10.6 >50 >50 24% 1.8
3h 3.09 >50 >50 37% 0.5 4n >50 >50 >50 n.d. n.d.
3i 5.01 >50 >50 51% 7.0 4o 12.0 >50 >50 25% 0.1
3j >50 >50 >50 n.d. n.d. 8a >50 >50 >50 n.d. n.d.
3k >50 >50 >50 n.d. n.d. 8b 31.3 >50 >50 10% −0.3
3l >50 >50 >50 n.d. n.d. 8c 21.1 >50 >50 5% 0.2
3m >50 >50 >50 n.d. n.d. 8d >50 >50 >50 n.d. n.d.
3n >50 >50 >50 n.d. n.d. 8e >50 >50 >50 n.d. n.d.
3o >50 >50 >50 n.d. n.d. 8f >50 >50 >50 n.d. n.d.
3p >50 >50 >50 n.d. n.d. 8g >50 >50 >50 n.d. n.d.
3q >50 >50 >50 n.d. n.d. 8h >50 >50 >50 n.d. n.d.
4a 19.3 >50 >50 19% 1.8 9 >50 >50 >50 n.d. n.d.
4b 13.0 >50 >50 16% 1.9 10a >50 >50 >50 n.d. n.d.
4c >50 >50 >50 n.d. n.d. 10b >50 >50 >50 n.d. n.d.
4d >50 >50 >50 n.d. n.d. 10c >50 >50 >50 n.d. n.d.
4e >50 >50 >50 n.d. n.d. 10d >50 >50 >50 n.d. n.d.
4f >50 >50 >50 n.d. n.d.
1KD values were determined by using surface plasmon resonance (SPR) experiments. 2 Relative TO displacement ratio (%) was determined at a fixed i-motif concentration (1 μM) either with or without a fixed concentration (5 μM) of compounds. 3 temperature enhancements (ΔTm) were determined using circular dichroism (CD)-melting experiments. ΔTm = Tm (DNA + ligand) − Tm (DNA). 4 No significant binding affinity was observed upon the addition of up to 50 μM compounds. 5 “n.d.” means not determined.
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Zeng, H.; Kang, S.; Zhang, Y.; Liu, K.; Yu, Q.; Li, D.; An, L.-K. Synthesis and Biological Evaluation of Oleanolic Acid Derivatives as Selective Vascular Endothelial Growth Factor Promoter i-Motif Ligands. Int. J. Mol. Sci. 2021, 22, 1711. https://doi.org/10.3390/ijms22041711

AMA Style

Zeng H, Kang S, Zhang Y, Liu K, Yu Q, Li D, An L-K. Synthesis and Biological Evaluation of Oleanolic Acid Derivatives as Selective Vascular Endothelial Growth Factor Promoter i-Motif Ligands. International Journal of Molecular Sciences. 2021; 22(4):1711. https://doi.org/10.3390/ijms22041711

Chicago/Turabian Style

Zeng, Huang, Shuangshuang Kang, Yu Zhang, Ke Liu, Qian Yu, Ding Li, and Lin-Kun An. 2021. "Synthesis and Biological Evaluation of Oleanolic Acid Derivatives as Selective Vascular Endothelial Growth Factor Promoter i-Motif Ligands" International Journal of Molecular Sciences 22, no. 4: 1711. https://doi.org/10.3390/ijms22041711

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