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Selected Aspects Related to Medicinal and Aromatic Plants as Alternative Sources of Bioactive Compounds

National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, 060021 Bucharest, Romania
Department of Science and Engineering of Oxide Materials and Nanomaterials, University “Politehnica” of Bucharest, 011061 Bucharest, Romania
Veterinary Medicine of Bucharest, University of Agronomic Sciences, 011464 Bucharest, Romania
Author to whom correspondence should be addressed.
Academic Editor: Raffaele Capasso
Int. J. Mol. Sci. 2021, 22(4), 1521;
Received: 13 January 2021 / Revised: 30 January 2021 / Accepted: 31 January 2021 / Published: 3 February 2021
(This article belongs to the Special Issue Biological Properties of Medicinal Plants)


Natural compounds obtained from different medicinal and aromatic plants have gained respect as alternative treatments to synthetic drugs, as well as raw materials for different applications (cosmetic, food and feed industries, environment protection, and many others). Based on a literature survey on dedicated databases, the aim of the present work is to be a critical discussion of aspects regarding classical extraction versus modern extraction techniques; possibilities to scale up (advantages and disadvantages of different extraction methods usually applied and the influence of extraction parameters); and different medicinal and aromatic plants’ different applications (medical and industrial applications, as well as the potential use in nanotechnology). As nowadays, research studies are directed toward the development of modern, innovative applications of the medicinal and aromatic plants, aspects regarding future perspectives are also discussed.
Keywords: medicinal plants; bioactive compounds; biomedical applications; industrial applications; nanotechnology medicinal plants; bioactive compounds; biomedical applications; industrial applications; nanotechnology

1. Introduction

Natural compounds obtained from different medicinal and aromatic plants (MAPs) have gained respect as alternative treatments, as well as raw material for different applications. Medicinal plants are a source of bioactive compounds that act as drugs in traditional treatments [1]; meanwhile, aromatic plants represent a rich source of essential oils, which can be used for their aroma and flavor [2]. MAPs are also used in cosmetics, functional food, or natural dyes production [3], thousands of species are all over the world being explored and exploited [4]. Another recent application is in the nanotechnology area, where the plant extracts’ phytoconstituents act as reducing and capping agents for the reduction of metallic ions from solutions in order to obtain different metallic nanoparticles, with further biomedical or industrial applications [5] (Figure 1).
The increased population led to a higher utilization of these plants, so their residues are proportional, with a huge amount of biomass generated as by-products [6], representing a growing market in the natural-based products [7]. The general use of MAPs all over the world is not homogenic, due to different factors: (i) in developed countries, even if the demand for natural treatments is high, profits of the growers and producers remain low because of the existing intermediaries which increase the price, as well as the lack of organization and networking by the poor collectors of medicinal plants from the wild; (ii) rigorous regulations and documentations requirements; and (iii) in less developed countries, there are poor traceability mechanisms from plant to population [8].
In this context, there are many studies reporting biological effects that can be attributed to MAPs or derived products, against several diseases, such as cancer, neurological, respiratory, inflammatory, cardiovascular diseases, and many others [9,10,11,12]. Using phytoconstituents or natural-based products as complementary treatments improved the oxidative status, which is due to their activity in compensating the inefficacy of the endogenous defense systems and in the enhancement of the overall antioxidant response [13,14]. Under stress conditions, the human body produces more reactive oxygen and nitrogen species (ROS/RNS) than enzymatic and non-enzymatic antioxidants, which is conducive to cell damage and health problems [15], and biological active products have a crucial role in combating oxidative stress.
In addition to traditional medical applications of medicinal and aromatic plants, there is the possibility of using them in cosmetic products, feed or food additives and preservatives, or as a viable tool for biotechnological applications, such as the enhancement of secondary metabolites by genetic engineering [16,17,18].
Based on a literature survey on dedicated databases (Scopus, ScienceDirect, SpringerLink, PubMed, Web of Science), the aim of this review paper is to be a critical discussion of aspects regarding classical extraction versus modern extraction techniques; possibilities to scale up (advantages and disadvantages of different methods usually used and the influence of extraction parameters); and different medicinal and aromatic plants’ different applications (medical and industrial applications, as well as the potential use in nanotechnology). In addition, future perspectives are critically discussed.

2. Classical Extraction Versus Modern Extraction Techniques: Possibilities to Scale up

Classical methods were developed during the time, such as a simple approach involving only water, herbs, and energy [19]. During the optimization process, the economic costs and energy necessary for obtaining different bioactive compounds tends to decrease, leading to higher yields of target compounds. Instead of maceration, decoction, or infusion, in the past, researchers developed methods such as Soxhlet, Clevenger, Kumagawa, or Likens–Nickerson simultaneous distillation–extraction, which, in turn, are replaced in the present day with modern equipment such as microwave-assisted extraction, supercritical fluid extraction [20], or ultrasound-assisted extraction [21]. Some examples are presented in Table 1 in order to present conditions for classical extraction versus necessary conditions for modern methods.
From the data presented in Table 1, it can be seen that for the same plant, extraction conditions may vary, depending on each method: for classical methods, the extraction time and the amount of solvent used are higher than for modern techniques compared to the obtained extraction yield (i.e., Satureja hortensis L. or Hippophae rhamnoides L.), thus leading to new approaches and perspectives. Recently, researchers proposed the introduction of a “Green Certificate”, which is based on weighted penalty points and the use of a color code in which reagent toxicity and volume, energy consumption, and the quantity of generated wastes (in the extraction step) are the main parameters used for the quantification of the “green” character [39,40,41]. All of these parameters contribute to a “greener character of the technique”, which is sometimes more important than the easiness of obtaining results. The classical approaches usually provide the lowest green certificate values, which is due to the energy and high amounts of solvents consumption. The “green certificate” is based on the application of a color code associated to a letter, class A being the “greenest” one [41]. The selection of a suitable solvent is crucial to improve the extraction yields, and moreover, the amount of resulted waste after the extraction. Polar solvents are commonly used, such as ethanol, methanol, and isopropanol [42], but the trends in “green chemistry” are going toward novel solvents with less toxicity as natural deep eutectic solvents (NADES) [43]. Espino and coworkers developed and optimized ultrasound-mediated extraction for phenolic compounds from Larrea cuneifolia Cav. 1800, which is a medicinal plant from the Larrea genus used in the Argentinian folk medicine [44]. They chemometrically optimized the extraction conditions, obtaining better results than using classical solvents in terms of resulted wastes: thus, for conventional techniques (maceration, decoction, heat reflux), wastes are in the range 1.5–2.85 penalty points for waste calculated according to the methodology previously mentioned [39,40,41], while for modern techniques, such as microwave and ultrasound-assisted extractions, wastes are in the range of 1–1.5 penalty points for waste, which are calculated according to the same protocol. These values of penalty points of the wastes offer a green certificate of class A (with a value in the range 100–90) and class B (89–80). Considering the results presented by the authors, the use of NADES and modern extraction techniques (such as ultrasound extraction) could be successfully applied for the scale-up of the procedure.
Another “green solvent” is carbon dioxide, which can be used as the main solvent for supercritical fluid extraction, having advantages such as non-toxicity and thermodynamic parameters, which facilitates its use in the supercritical state [45], being a suitable solvent first for non-polar molecules (lipids, terpenes, etc.), followed by more polar molecules [46]. In the case of Salvia officinalis L., Jokić and coworkers optimized a supercritical CO2 extraction method for terpenes and phenolic compounds, obtaining increased yields of recovery (7.4%) by varying the utilized pressure (15 or 20 MPa) [33] in only 90 min of extraction; meanwhile, Miguel and collaborators, through hydro-distillation obtained in 180 min a decreased yield of recovery (2%) [34]. Moreover, the quality of the obtained compounds can be modified depending on the extraction method used; Ollanketo and coworkers demonstrated that pressurized hot water extraction is a highly promising alternative to conventional solid–liquid techniques, in terms of final application of the recovered compounds; the highest antioxidant activities did not correspond to the maximum recovery yields, but the antioxidant activity was highest when pressurized hot water (PHW) was used as the extracting solvent instead of the maceration method [35]. In this case, the quality of the compounds is influenced by the extraction conditions, in which an increased time and temperature lead to a decrease of biological effects of the obtained compounds. In addition, some compounds do not respond to a classical or modern solvent (such as NADES) extraction, and it is necessary to optimize an enzymatic process and break hydrogen or hydrophobic bonding, which keeps them trapped in the polysaccharide–lignin network [47].
As a pro argument for modern extraction techniques, in addition to the reduced costs and energy, there is the use of a cascade of different solvents, which is due to the existence of a complex matrix of MAPs; this approach is conducive to the recovery of a large range of bioactive compounds. This is the case of Algerian Thymus munbyanus Boiss. & Reut., 1852, where acetone, ethanol, and water were used in successive pressurized extractions [38]. The recuperation of the solvents, especially the toxic ones such as acetone, can be performed under vacuum conditions, removing the possibility of contamination on the environment or on the final products. In addition to oxygenated monoterpenoids, such as camphor (11.7%) and geraniol (7.5%), and sesquiterpenoids and monoterpenoids, such as (E)-nerolidol (13.7%), terpinen-4-ol (10.6%), and camphor (7.6%), researchers also obtained geranyl acetate (6.3%) and β-terpinyl acetate (5.1%), caryophyllene oxide (5.1%) and borneol (5.6%), respectively β-terpinyl acetate (4.8%) and linalool (4%).
A proper balance in choosing the methods and setting operational parameters could lead to an optimized process, obtaining different compounds from a plant matrix, which in other conditions would be damaged or could not be obtained [48]. The structure of target molecules can influence their solubility at different conditions of high pressure; in extreme conditions, interactions and aggregations or even their re-adsorption are possible. Using moderate conditions such as medium pressures or temperatures automatically can result in lower costs and energies, which is beneficial for all the production and processing chain. Moreover, for modern extraction methods, for which it is not necessary to use organic or toxic solvents, the obtained bioactive compounds could achieve, in certain conditions, a “green characteristic” and can be used in further applications such as the design of foods with improved functionality or medical care.
Modern extraction methods have attracted a great amount of interest in the last few years, especially due to their scale-up possibilities and their ability to provide superior quality extracts with economic benefits; a scientific and analytical approach must be conducted for this step. The classical extraction method can be also scaled up, but factors such as instrumentation, batch/flow process, kinetics, economics, energy consumption, and amounts of wastes tilt the balance toward the scale up of non-conventional extraction techniques, such as microwaves or ultrasonic-assisted, supercritical fluid, negative cavitation, pressurized fluid, etc. [49]. In some cases, the adequate parameters for lab scale can be used in scale-up process [50], but in other cases, maintaining the same conditions can led to a decreased recovery yield when the scaled-up method is applied [51]. In the optimization process, there must be a balance between the parameters, and according to Belwal and coworkers, every method must prove its maturity level through technology readiness levels (TRL) [49].

3. The Influence of Extraction Conditions

For each method used as an extraction technique, optimization of the parameters is mandatory. The reaction parameters that can influence the extraction process are the used solvent, temperature, ratio of vegetal material/amount of solvent, pH, extraction time, and factors related to the raw material matrix [52].
According to the experiments performed by different authors, the most suitable solvents for bioactive compounds extraction are water for anthocyanins, phenolic acid, saponins, terpenoids recovery [53,54,55,56], and alcohols such as methanol or ethanol, alone or as mixtures, for anthocyanins, phenolic acids, flavonoids, tannins, saponins, or terpenoids recovery [57,58]. Alcohols have the property of increasing cell permeability by affecting the phospholipid bilayer of the membrane, permitting a good transfer of bioactive compounds into the solvent. In addition, the water/alcohol mixtures permit a good recovery yield, especially for phenolic compounds, which have a good solubility, due to the alcohol presence (Figure 2).
In the last years, the application of neoteric solvents received special interest, in order to minimize the use of toxic components and maximize the extraction efficiencies, with an emphasis on reducing their toxicity after usage [59]. This category includes ionic liquids and deep (natural) eutectic solvents, which were used due to their possibility toward tailored-extraction, thus increasing extraction efficiency for complex matrixes. Ionic liquids are suitable for different bioactive compounds, such as alkaloids [60] or phenolic compounds [61] due to the good miscibility of target compounds with the solvent. Eutectic solvents have the same properties with ionic liquids, and beside this, they are less toxic and more biodegradable [62]. In addition, bio-based solvents received special interest, such as 2-methyltetrahydrofuran, limonene, or 2-methyltetrahydrofuran, successfully replacing solvents such as n-hexane and toluene with lower production costs and increased biodegradability [63]. Some of these solvents are not commercially available, and they are not still used at an industrial level. Nowadays, the experiment must be carried out with respect to the balance between environment and production costs, in order to achieve full sustainability in the process implementation.
Depending on the plant matrix, temperature is another important parameter that is necessary in the optimization process. Increased temperature can lead to a higher solubility of the analyte, but at the same time, it can lead to the degradation of thermo-sensitive compounds [64]. In the processes where neoteric solvents are used, the temperature significantly modifies the properties of the solvent, thus modifying the solubility of the analytes [65].
The role and optimization of time, pH, solid–liquid ratio, or pressure are discussed in different papers [66,67,68,69,70], as these are significant parameters that can influence the extraction efficiency. Moreover, it cannot be stated than one parameter is more important than another, each of them having a tremendous influence on the overall extraction process results.

4. Different Medicinal and Aromatic Plants—Different Applications

4.1. Medical Applications

Due to the fact that this review paper intends to be a critical discussion on different aspects related to the applications of medicinal and aromatic plants even though they are used as such, or as extracts, nanomaterials, or purified bioactive compounds, it is impossible to approach this topic for all the existing plants. We will focus on a few examples of medicinal and aromatic plants, with a wide spread and knowledge, based on the newest reports, in order to demonstrate the importance of these plants for treating/preventing diseases of the century (cardiovascular or neurodegenerative diseases, diabetes, etc.) or even for simple biological effects (antibacterial, antioxidant).

4.1.1. Origanum spp.

Oregano belongs to the Lamiaceae family, and within the genus Origanum, there are three groups, 10 sections, 38 species, 6 subspecies, and 17 hybrids [71]. Among the different Origanum species, several are commonly used for culinary purposes (especially as spices), such as the Greek oregano (Origanum vulgare L. ssp. hirtum (Link) Ietswaart), Marjoram (Origanum marjorana L.), Turkish oregano (Origanum onites L.), round-leaved oregano (Origanum rotundifolium L.), or Syrian oregano (Origanum syriacum L.) [72,73,74]. The Origanum spp. plants, especially leaves, are rich in terpenes (monocyclic terpenes—from which the most concentrated are carvacrol and thymol; bicyclic monoterpenes such as thujene, sabinene, camphene, α and β pinene; acyclic monoterpenes such as linalool; sesquiterpenes such as β-bisabolene; triterpenoids such as ursolic and oleanolic acids) [75] and phenolic compounds (hydroquinone and derived compounds; phenolic acids: p-hydroxybenzoic, vanillic, syringic; flavonoids) [72], but the composition depends on different factors (species, cultivation area, environment conditions, harvesting time, phenopase, etc.).
Even if it is used as such or encapsulated in different matrixes, the oregano essential oil (OEO) has predominant medical applications. In the last few years, researchers reported in vitro antibacterial, antioxidant, and anti-inflammatory activities for the essential oil [76,77,78,79], in the year 2020, Khan and co-workers reported the results of an in vivo study regarding a wound healing based on capsules of co-polymer of poly (L-lactide-co-caprolactone) (PLCL)/silk fibroin loaded with different concentrations of OEO [80]. The activity of oregano essential oil can balance the production of ROS, playing a positive role in the healing process. In addition, it has the property of boosting the granulation, re-epithelialization, better organization of collagen fibers, as well as capillary network formation. Avola and coworkers described biological effects of OEO in the restoring of physiological cell homeostasis, using human keratinocytes NCTC 2544 treated with interferon-gamma (IFN-γ) and histamine (H) [81]. OEO diminished the release of a variety of pro-inflammatory mediators such as iNOS (inducible nitric oxide synthase), ICAM-1 (inter-cellular adhesion molecule 1), and COX-2 –(cyclooxygenase-2), reducing ROS development, 8-OHdG (8-hydroxy-2′-deoxyguanosine) formation, and maintaining the protein proliferating cell nuclear antigen (PCNA) expression without influencing cell viability. The OEO was proven to play an important role in preserving the extracellular matrix components, remodeling, and tissue healing. For re-epithelialization of the skin after its injury, OEO has the property of improving NCTC 2544 cell proliferation. In this study, the beneficial effects were attributed to carvacrol, which is the main constituent in OEO.
OEO has also a beneficial effect when it is encapsulated in polymeric matrixes, and different studies being conducted toward both developing new materials that can limit the volatility of the essential oil and obtaining a controlled release for the topical application. This is the case of nanocomposite films based on poly(vinyl alcohol) (PVA) and alphachitin nanocrystals (α-CHNC, from shrimp and lobster), which are conductive to a “green” character of the treatment [82]. In general, one of the main drawbacks of the application of essential oils in wound dressing is related to their very low solubility or absent solubility in aqueous solutions; cross-linking and/or other additives are usually necessary [82].
One of the major health problems in the world is the increased mortality for metabolic diseases and diabetes mellitus [83]. OEO is a solution for antihyperglycemic activity, being a good candidate to prevent and/or treat diseases arising from oxidative stress, such as diabetes mellitus, by the inhibition of α-amylase and α-glucosidase [84]. For this effect, the responsible phytoconstituent is 4-terpineol, which was found to be the most abundant compound in OEO; the recorded inhibition of α-amylase activity was 81.4%, while the inhibition of α-glucosidase activity was 50.5%, which is within the results range obtained in other studies [77].
OEO is known as one of the best candidates to be used as preservatives against the Bacillus species (associated with food spoilage), which is a property attributed to the presence of oxygenated terpenes (terpenoids) [85] or in association with other treatments, such as gamma irradiation, as well as against different pathogens (Escherichia coli, Salmonella typhimurium, and Listeria monocytogenes) [86]. Moreover, some review papers present recent developments regarding the use of OEO and nanomaterials based on oregano extracts in medical care or other applications, such as the food industry [87,88,89]. OEO can reduce the water vapor permeability (WVP) and enhance the contact angle, transparency, and moisture value of final films containing it, which is necessary for the development of different coatings.

4.1.2. Thymus spp.

Thymus genus, belonging to the Lamiaceae family, consists of 250–350 taxa widespread all over the world. Among these species, Thymus vulgaris L. 1753 is the most commonly found, and it is used as a culinary product or as medicinal remedy. The plant is rich in essential oil (the most abundant in flowers), which has as its main chemical classes terpenes, terpene alcohols, phenolic derivatives, ketones, aldehydes, ethers, and esters [90]. Depending on the species, the chemical composition can vary: monoterpene phenols, with the isomers thymol and carvacrol, are usually two major compounds in most species (T. vulgaris, T. capitatus, T. pulegioides, T. pubescence, T. daenensis, T. transcaspicus, T. serpyllum, T. fallax, T. kotschyanus, T. reolutus Celak) [91], while T. caespititius, T. camphoratus, and T. mastichina are rich in α-terpineol, linalool, and 1,8 cineole [92]; T. algeriensis has α-pinen as its main constituent [93], and geraniol is specific for the Romanian native T. glabrescens [94].
In order to eradicate pathogenic yeasts strains, such as Candida albicans, C. krusei, or C. glabrata, Muslim and Hussin proposed a recipe based on the synergistic effects of Thymus kotschanus Boiss & Hohen essential oil and ketoconazole for decreasing the incidence of candidiasis among vulnerable individuals with underlying diseases (antibiotic therapy, AIDS, etc.) [95]. In this case, the role of the essential oil was to inhibit the microbial population and to increase the permeability of fungal membranes, making them more sensitive to other antifungal agents, such as synthetic drugs. As a perspective of using EO for downexpressing the Als (Agglutinin-like sequence), a biofilm-associated gene is the development of future in vivo studies to optimize the effects of temperature, pH, host responses, as well as drug resistance to these EOs.
In 2020, Najafloo and coworkers published a review paper that presented the applications in the area of antibacterial wound dressing of thymol-incorporated materials, based on essential oil’s therapeutic properties: antioxidant, anti-inflammatory, local anesthetic, antinociceptive, cicatrizing, antiseptic, and particularly antibacterial and antifungal [96]. These delivery systems can be formed by lipid-based nanocarriers, polymer-based nanoparticles, fiber capsules, or hydrogels, all of them having application in wound treatment, the active bio-compound thymol, having the property to modulate the release production of reactive species, including nitric oxide, TNF-α, and IL-1β cytokines and growth factors such as TGF-1β, thus stimulating re-epithelialization, angiogenesis, and the formation of granulation tissue.
In addition, terpenes extracted from the aerial parts of Thymus spp. are potent cardioprotective agents, protecting against biochemical and histopathological changes in the heart tissue of animals by restoring the activities of endogenous antioxidant enzymes [97]. At heart diseases, there is an increased generation of reactive oxygen species such as superoxide anion (O2) and hydroxyl radicals (OH), and thymol has the ability to remove the damage of ROS, increasing the endogenous antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), or glutathione-s-transferase (GST). Other applications to be further developed using the main constituents of Thymus spp. in different forms can be beneficial in biomaterials development and tissue regeneration; recent studies [98,99] present the antiviral potential of medicinal and non-medicinal plants applied for the contemporary pandemic agent COVID-19; considering the proven biomedical potential of Thymus spp., their application in this area should definitely be explored.

4.1.3. Salvia spp.

Salvia is the largest genus of the Lamiaceae family, containing over 900 species, and beside the therapeutical properties that confer the possibility of being used in medical applications, it can be used as a flavoring agent, perfume additive, and condiment [100]. All parts of the plant are rich in antioxidant and antimicrobial phytoconstituents, such as essential oils, terpenes, flavonoids, phenolic acid, and steroids. In addition to leaves and flowers, which can be used from most of the plants, the roots have beneficial properties, and moreover, the roots of Salvia miltiorrhiza Bunge are a good instrument for biotechnological applications, such as the enhancement of secondary metabolites by genetic engineering and elicitor treatment [101].
The anxiolytic effect of a Salvia extract was studied by Lin and coworkers, using the elevated plus-maze test (EPM) and the hole-board test (HBT) [102]. In the cited study, the application of Salvia extract on mice showed a significant increase in their head-dip counts and duration compared to the control group (diazepam and flumazenil), recommending this plant as a potential anti-anxiety drug.
In addition, the combination of Salvia miltiorrhiza Bunge. and Carthamus tinctorius L. extracts acts synergistically, producing a combined effect, enhanced compared with the individual effects in targeting complex diseases such as diabetes mellitus, hypertension, and related cardiovascular diseases [103]. Obesity, hypertension, and diabetes share a common pathological relationship with metabolic syndrome and from one chronic state, it can be transformed to another. The mixture of the compounds has the ability to inhibit the formation of acellular capillaries (which can be related to the development of vascular aneurysms), as well as regulator effect on glucokinase induction, AMPK-α/phosphorylated AMPK-α, insulin receptor substrate-1, and PPARγ, thus mediating the glucose metabolism.
As a future perspective, the pharmacokinetics and pharmacodynamics of the natural compounds recipe require further preclinical and clinical investigation in order to be applied at a large industrial scale as natural drugs.

4.2. Industrial Applications

In addition to medical applications, bioactive compounds from medicinal and aromatic plants proved to have important properties, which made them good candidates for industrial applications. Due to their antioxidant and antimicrobial effects, these natural products can be used especially in the food, pharmaceutical, and perfume industries (Table 2).
Even from the beginning of the 1990s, the interest in using natural compounds in industrial applications started in controlling the postharvest disease of citrus fruits [104] and continued until nowadays, using edible coatings (edible chitosan coatings incorporated with Thymus capitatus essential oil) as an alternative method to prolong the shelf life of perishable fruits (strawberries) [105]. The treatment can be applied to postharvest fruits to keep them in good conditions for more than 15 days, and their antioxidant properties are not affected. Jemaa and coworkers applied as a food preservative the essential oil of Thymus capitatus, the antimicrobial activity being attributed to carvacrol [106]. Eryngium campestre essential oil encapsulated in chitosan nanoparticles was developed to reduce the microbial counts and to extend the shelf life of sweet cherries, farnesene being identified as the main responsible compounds for the antimicrobial effects [107]. For the cases of encapsulation, different physicochemical characterizations are needed in order to optimize these materials for specific applications.
In the food industry, natural compounds can be used as natural packaging materials, which have the ability to increase the shelf life of meat products. Encapsulated in chitosan particles, these compounds can protect against different bacteria (Pseudomonas spp., Listeria monocytogenes, etc.), and moreover, they have the property to inhibit lipid oxidation [108]. Poly lactic acid/nanochitosan composite film enriched with Polylophium involucratum was developed for prolonging the shelf life of chicken fillets during refrigerated storage for 10 days [109]. These packaging films used for removing adverse sensorial properties due to microbial attack are environmentally friendly materials that are beneficial for human health, replacing petroleum-based plastic packaging. Rehman et al. presented different plants such as Syzygium aromaticum, Mentha piperita, Salvia rosmarinus, or Eucalyptus globulus as potential natural sources for food packaging [110].
In addition to these applications, there are edible coatings, which are considered as a part of the final product, and they are used to increase the oxidative stability during the storage of different foods; this approach is considered a preservation technique. Hosseini and coworkers developed a recipe based on whey protein concentrate, carboxymethyl cellulous, glycerol, and rosemary extract to improve the color and oxidative properties of the stored sunflower seeds [111]. By adding carboxymethyl cellulose, the synergistic effect with the extract prevents the formation of hydroperoxides and conjugated dienes [112], and in the case of sunflower seeds, it provides the highest tensile strength, coating percentage, and elongation of the film [111].
With a special respect to the natural products recipe, a great development started in the last few years for natural insecticidal recipes, which are less harmful to humans and the environment. Th. alternans and T. montanum subsp. Jailae were studied by Pavela and coworkers as insecticide against Musca domestica L., Culex quinquefasciatus Say, and Spodoptera littoralis (Boisd.) [113]. The insecticidal effect can be attributed to the synergistic effect of the individual compounds found in these species, and further studies are needed in order to elucidate the mechanism of action.
In addition, MAPs, such as Origanum vulgare subsp. Hirtum, Foeniculum vulgare Mill, Pimpinella anisum L., and their extracts can be used as a novel feed additive in turkey production, having beneficial effects, such as food microbial safety or even can enhance production performance [114] as “green pesticides” to limit the use of hazardous synthetic pesticides (the case of Heracleum persicum and Achillea millefolium essential oil against Plodia interpunctella, citronellal from Cymbopogon winterianus against Spodoptera frugiperda larvae, or rosemary oil applied against Agriostes obscurus larvae) [115] or in the zootechnological field to enhance animals’ performance and health [116]. Moreover, purified bioactive compounds from MAPs (such as Calendula officinalis L., Lavandula vera DC, Artemisia absinthium L.) or even plant extracts (as a whole) have potential applications in the cosmetic industry, which nowadays is focused on new technologies and explores alternative sources of raw materials; the trends in this direction are based on the use of plant-origin components having polyfunctional properties and long-lasting effects [117]. In this domain, there is ongoing in-depth research for identifying natural resources for sunscreen cosmetics; bioactive compounds such as green tea polyphenols, Rosa damascene flower extracts, aromatic compounds isolated from lichens, flowering tops of Dracocephalum moldavica and Viola tricolor, and aromatic and flavonoid compounds from saffron, Crocus sativus, were evaluated for their effects [118]. In addition, compounds such as a-pinene, b-pinene, limonene, cymene, linalool, cis-2-methoxycinnamic acid, and cinnamaldehyde are used for their anti-melanogenic and anti-aging properties [119,120].
Instead of synthetic additives, which are toxic and harmful to humans and the environment, the interest regarding the application of natural extracts from plants in this area has increased. These substances can be successfully applied as additives in steel production for developing corrosion-resistant materials [121]. The beneficial effects of the newly developed coatings can be increased by decreasing the cost of production, natural compounds being a cheap raw material, but the disadvantage of their use is over the long term, being degradable under specific conditions (UV light, the possibility of oxidizing or evaporating) [122]. Coatings based on bioactive compounds from MAPs can also have antimicrobial [123,124] or antifouling applications [125].
Natural products can be used as a natural resource of photostabilizers in coatings, instead of hindered amine light stabilizers or inorganic nanoparticles, which have the disadvantage of leaching. Tannin from Pinus brutia Ten. has improved the color stability and surface quality of the coated wood during artificial weathering [126] and coatings based on Chinese fir bark extract enhanced the weathering resistance of wood [127].

4.3. Applications in Nanotechnology

In the last decades, nanotechnology has offered a series of valuable tools for improving our daily life. Within this area, the application of different plants (including MAPs) led to the development of a new research field, nanoparticles phytosynthesis. As a general rule, the plant’s phytoconstituents, acting as both reducing and capping agents, determine the final morphology and size of the obtained nanoparticles as well as contribute to an increase in biological activity and a reduction of potential toxicity. Our group previously reviewed the most recent findings in the application of phytosynthesized NPs as antimicrobial and antitumoral agents, as well as the results regarding their toxicological potential [133], plants such as Azadirachta indica A. Juss., Berberis vulgaris L., Coriandrum sativum L., Mentha pulegium L., Myrtus communis L., Salvia hispanica L., or Tribulus terrestris L. (just to name few examples) offer proper extracts to obtain silver, gold, or ZnO nanoparticles. Moreover, these nanoparticles, in particular those phytosynthesized with the help of medicinal plants such as Artemisia vulgaris L., can find application in other medical applications, such as antiviral agents for chikungunya virus [134], antiparasitic activity against yellow fever mosquito (dengue fever vector) [135], or against different infections [136].
The subject of phytosynthesized nanoparticles is very vast and itself is a main topic for a review paper. For this manuscript, we focused on the discussion of some important aspects related of the general mechanism of action and applications in some domains related to those ones in which “bioactive compounds from MAPs” can be used.
As previously stated, the plant extracts constituents act as reducing agents for metallic salts, also having the role as stabilizing/capping agents and inducing the polymorphism of the obtained nanoparticles. Moreover, the effect of nanoparticles is enhanced by the synergistic effect of bioactive compounds, especially in antimicrobial and antioxidant applications [137].
The mechanism of cellular action of these nanoparticles is similar for several types of application: the nanoparticles have the ability to inhibit the cellular mechanism by DNA damage, which is conducive to the death of the target cell. In addition, the formation of reactive oxygen species, including hydrogen peroxide, leads to oxidative stress and subsequent cell damage [138]. The phytosynthesized nanoparticles decrease the glycated hemoglobin antioxidant defense, presenting and reducing the metalloproteinases activity that is conducive to an anti-inflammatory effect [139]. Functionalizing nanoparticles with natural antioxidants increases their stability and biocompatibility [140].
In addition to medical applications (antimicrobial, antioxidant, antitumoral, etc.), “green nanoparticles” are used for environmental applications and for waste water depollution as “green catalysts” [141,142].

5. Conclusions and Future Perspectives

The use of medicinal and aromatic plants has strong roots in the traditional medicine since antiquity, from the treatment of minor illnesses to more contemporary diseases [143]. Since then, evolving from simple maceration and decoction, based on modern approaches, research has reached a top level of technologicalization in which bioactive compounds are successfully recovered or even purified [144]. Our group presented in a published paper the most recent findings and approaches for the recovery of phytoconstituents from MAPs [1], so now, we will focus for this paper on future perspectives of using these compounds for further applications.
One of the most spectacular uses of MAPs is in the biotechnological domain, where omics technologies have a crucial role. For an increased production of natural bioactive compounds, the elucidation of the biosynthetic pathway will help directly and efficiently obtain active compounds of different plants in hairy roots systems. In addition, a good knowledge and usage of biotic and abiotic elicitors can dramatically improve the production of targeted active compounds. If some studies proposed an exact methodology and models for the biosynthesis and regulation of active compounds (for example, in Salvia miltiorrhiza Bunge., as previously presented), further studies are needed for other medicinal plants and for similar developments.
The road “from plant to pharmacy shelf” is long, sinuous, and hardly achievable, but with tremendous rewards at the end: the development of commercial products that could increase the life quality and treat a series of illnesses and conditions with high social and economic impact on the society as a whole.
Knowing the beneficial effect of metallic nanoparticles in the medical applications and following the model of chemical synthesized Fe3O4 magnetic nanoparticles that have effects in neurodegenerative diseases [145], further research studies are needed for obtaining “green” metallic nanoparticles and nanomaterials, using the natural extracts. In addition, for removing the actual drawbacks of drug-transporting vectors (such as phospholipids degradation, quick systemic exclusion, inadequate stability under prolonged storage, moderate efficiency for entrapping lipophilic compounds) [146], further in-depth studies are required. In addition to medical applications, phytosynthesized metallic nanoparticles could be applied as biosensors for the food industry or environment protection, offering on-site monitoring and providing real-time data, replacing the expensive equipment and sample processing, and providing the foundation for the development of next-generation catalysts or other important industrial applications.

Author Contributions

All authors contributed to the present work. All authors have read and agreed to the published version of the manuscript.


This research was funded through the project SusMAPWaste, SMIS 104323, Contract No. 89/09.09.2016, from the Operational Program Competitiveness 2014–2020, project co-financed from the European Regional Development Fund.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

No new data were created or analyzed in this study. Data sharing is not applicable to this article.

Conflicts of Interest

The authors declare no conflict of interest.


  1. Fierascu, R.C.; Fierascu, I.; Ortan, A.; Georgiev, M.I.; Sieniawska, E. Innovative approaches for recovery of phytoconstituents from medicinal/aromatic plants and biotechnological production. Molecules 2020, 25, 309. [Google Scholar] [CrossRef] [PubMed]
  2. Manousi, N.; Sarakatsianos, I.; Samanidou, V. Extraction techniques of phenolic compounds and other bioactive compounds from medicinal and aromatic plants. In Engineering Tools in the Beverage Industry. Volume 3: The Science of Beverages; Grumezescu, A.M., Holban, A.M., Eds.; Woodhead Publishing: Duxford, UK, 2019; pp. 283–314. [Google Scholar]
  3. Saha, A.; Basak, B.B. Scope of value addition and utilization of residual biomass from medicinal and aromatic plants. Ind. Crops Prod. 2020, 145, 111979. [Google Scholar] [CrossRef]
  4. Zengin, G.; Mollica, A.; Aumeeruddy, M.Z.; Rengasamy, K.R.; Mahomoodally, M.F. Phenolic profile and pharmacological propensities of Gynandriris sisyrinchium through in vitro and in silico perspectives. Ind. Crops Prod. 2018, 121, 328–337. [Google Scholar] [CrossRef]
  5. Fierascu, I.; Georgiev, M.I.; Ortan, A.; Fierascu, R.C.; Avramescu, S.M.; Ionescu, D.; Sutan, A.; Brinzan, A.; Ditu, L.M. Phyto-mediated metallic nano-architectures via Melissa officinalis L.: Synthesis, characterization and biological properties. Sci. Rep. 2017, 7, 12428. [Google Scholar] [CrossRef]
  6. Saha, A.; Tripathy, V.; Basak, B.B.; Kumar, J. Entrapment of distilled palmarosa (Cymbopogon martinii) wastes in alginate beads for adsorptive removal of methylene blue from aqueous solution. Environ. Prog. Sustain. Energy 2018, 37, 1942–1953. [Google Scholar] [CrossRef]
  7. Elfalleh, W.; Kirkan, B.; Sarikurkcu, C. Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey. Ind. Crops Prod. 2019, 127, 212–216. [Google Scholar] [CrossRef]
  8. Volenzo, T.; Odiyo, J. Integrating endemic medicinal plants into the global value chains: The ecological degradation challenges and opportunities. Heliyon 2020, 6, e04970. [Google Scholar] [CrossRef]
  9. Bazak, R.; Houri, M.; Achy, S.E.; Hussein, W.; Refaat, T. Passive targeting of nanoparticles to cancer: A comprehensive review of the literature. Mol. Clin. Oncol. 2014, 2, 904908. [Google Scholar] [CrossRef]
  10. Chen, R.J.Y.; Jinn, T.R.; Chen, Y.C.; Chung, T.Y.; Yang, W.H.; Tzen, J.T.C. Active ingredients in Chinese medicines promoting blood circulation as Na+/K+-ATPase inhibitors. Acta Pharmacol. Sin. 2011, 32, 141–151. [Google Scholar] [CrossRef]
  11. Pacher, P.; Steffens, S.; Haskó, G.; Schindler, T.H.; Kunos, G. Cardiovascular effects of marijuana and synthetic cannabinoids: The good, the bad, and the ugly. Nat. Rev. Cardiol. 2018, 15, 151–166. [Google Scholar] [CrossRef]
  12. Chandrashekhar, V.M.; Ranpariya, V.L.; Ganapaty, S.; Parashar, A.; Muchandi, A.A. Neuroprotective activity of Matricaria recutita L. against global model ofischemia in rats. J. Ethnopharmacol. 2010, 127, 645–651. [Google Scholar] [CrossRef] [PubMed]
  13. Berger, R.G.; Lunkenbein, S.; Ströhle, A.; Hahn, A. Antioxidants in food: Mere myth or magic medicine? Crit. Rev. Food Sci. 2012, 52, 162–171. [Google Scholar] [CrossRef] [PubMed]
  14. Bouayed, J.; Bohn, T. Exogenous antioxidants—double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid. Med. Cell. Longev. 2010, 3, 228–237. [Google Scholar] [CrossRef] [PubMed]
  15. Bessada, S.M.F.; Barreira, J.C.M.; Oliveira, M.B.P.P. Asteraceae species with most prominent bioactivity and their potential applications: A review. Ind. Crops Prod. 2015, 76, 604–615. [Google Scholar] [CrossRef]
  16. El-Sayed, S.M.; Youssef, A.M. Potential application of herbs and spices and their effects in functional dairy products. Heliyon 2019, 5, e01989. [Google Scholar] [CrossRef]
  17. Faccio, G. Plant complexity and cosmetic innovation. IScience 2020, 23, 101358. [Google Scholar] [CrossRef]
  18. Jha, P.; Sen, R.; Jobby, R.; Sachar, S.; Bhatkalkar, S.; Desai, N. Biotransformation of xenobiotics by hairy roots. Phytochemistry 2020, 176, 112421. [Google Scholar] [CrossRef]
  19. Rassem, H.H.A.; Nour, A.H.; Yunus, R.M. Techniques for extraction of essential oils from plants: A review. Aust. J. Basic Appl. Sci. 2016, 10, 117–127. [Google Scholar]
  20. Frohlich, P.C.; Santos, K.A.; Palú, F.; Cardozo-Filho, L.; da Silva, C.; da Silva, E.A. Evaluation of the effects of temperature and pressure on the extraction of eugenol from clove (Syzygium aromaticum) leaves using supercritical CO2. J. Supercrit. Fluids 2019, 143, 313–320. [Google Scholar] [CrossRef]
  21. Saleh, I.A.; Vinatoru, M.; Mason, T.J.; Abdel-Azim, N.S.; Aboutabl, E.A.; Hammouda, F.M. A possible general mechanism for ultrasound-assisted extraction (UAE) suggested from the results of UAE of chlorogenic acid from Cynara scolymus L. (artichoke) leaves. Ultrason. Sonochem. 2016, 31, 330–336. [Google Scholar] [CrossRef]
  22. Kimbaris, A.C.; Siatis, M.G.; Daferera, D.J.; Tarantilis, P.A.; Pappas, C.S.; Polissiou, M.G. Comparison of distillation and ultrasound-assisted extraction methods for the isolation of sensitive aroma compounds from garlic (Allium sativum). Ultrason. Sonochem. 2016, 13, 54–60. [Google Scholar] [CrossRef] [PubMed]
  23. Périno-Issartier, S.; Zill-e-Huma; Abert-Vian, M.; Chemat, F. Solvent free microwave-assisted extraction of antioxidants from sea buckthorn (Hippophae rhamnoides) food by-products. Food Bioproc. Technol. 2011, 4, 1020–1028. [Google Scholar]
  24. Cvetanović, A.; Švarc-Gajić, J.; Zeković, Z.; Gašić, U.; Tešić, Z.; Zengin, G.; Mašković, P.; Mahomoodally, M.F.; Đurović, S. Subcritical water extraction as a cutting edge technology for the extraction of bioactive compounds from chamomile: Influence of pressure on chemical composition and bioactivity of extracts. Food Chem. 2018, 266, 389–396. [Google Scholar] [CrossRef] [PubMed]
  25. Harbourne, N.; Jacquier, J.C.; O’Riordan, D. Optimisation of the extraction and processing conditions of chamomile (Matricaria chamomilla L.) for incorporation into a beverage. Food Chem. 2009, 115, 15–19. [Google Scholar] [CrossRef]
  26. Vian, M.A.; Fernandez, V.; Visinoni, F.; Chemat, F. Microwave hydrodiffusion and gravity, a new technique for extraction of essential oils. J. Chromatogr. A 2008, 1190, 14–17. [Google Scholar] [CrossRef]
  27. Patonay, K.; Szalontai, H.; Csugány, J.; Szabó-Hudák, O.; Kónya, E.P.; Németh, E.Z. Comparison of extraction methods for the assessment of total polyphenol content and in vitro antioxidant capacity of horsemint (Mentha longifolia (L.). J. Appl. Res. Med. Aromat. Plants 2019, 15, 100220. [Google Scholar] [CrossRef]
  28. Fornari, T.; Ruiz-Rodriguez, A.; Vicente, G.; Vázquez, E.; García-Risco, M.R.; Reglero, G. Kinetic study of the supercritical CO2 extraction of different plants from Lamiaceae family. J. Supercrit. Fluids 2012, 64, 1–8. [Google Scholar] [CrossRef]
  29. Binello, A.; Orio, L.; Pignata, G.; Nicola, S.; Chemat, F.; Cravotto, G. Effect of microwaves on the in situ hydrodistillation of four different Lamiaceae. Compt. Rendus Chim. 2014, 17, 181–186. [Google Scholar] [CrossRef]
  30. Wellwood, C.R.L.; Cole, R.A. Relevance of carnosic acid concentrations to the selection of rosemary, Rosmarinus officinalis (L.), accessions for optimization of antioxidant yield. J. Agric. Food Chem. 2004, 52, 6101–6107. [Google Scholar] [CrossRef]
  31. Carvalho, R.N.; Moura, L.S.; Rosa, P.T.V.; Meireles, M.A.A. Supercritical fluid extraction from rosemary (Rosmarinus officinalis): Kinetic data, extract’s global yield, composition, and antioxidant activity. J. Supercrit. Fluids 2005, 35, 197–204. [Google Scholar] [CrossRef]
  32. Rodríguez-Rojo, S.; Visentin, A.; Maestri, D.; Cocero, M.J. Assisted extraction of rosemary antioxidants with green solvents. J. Food Eng. 2012, 109, 98–103. [Google Scholar] [CrossRef]
  33. Jokić, S.; Molnar, M.; Jakovljević, M.; Aladić, K.; Jerković, I. Optimization of supercritical CO2 extraction of Salvia officinalis L. leaves targeted on oxygenated monoterpenes, α-humulene, viridiflorol and Manool. J. Supercrit. Fluids 2018, 133, 253–262. [Google Scholar] [CrossRef]
  34. Miguel, G.; Cruz, C.; Faleiro, M.L.; Simões, M.T.F.; Figueiredo, A.C.; Barroso, J.G.; Pedro, L.G. Salvia officinalis L. essential oils: Effect of hydrodistillation time on the chemical composition, antioxidant and antimicrobial activities. Nat. Prod. Res. 2011, 25, 526–541. [Google Scholar] [CrossRef] [PubMed]
  35. Ollanketo, M.; Peltoketo, A.; Hartonen, K.; Hiltunen, R.; Riekkola, M.L. Extraction of sage (Salvia officinalis L.) by pressurized hot water and conventional methods: Antioxidant activity of the extracts. Eur. Food Res. Technol. 2002, 215, 158–163. [Google Scholar] [CrossRef]
  36. Mašković, P.; Veličković, V.; Mitić, M.; Đurović, S.; Zeković, Z.; Radojković, M.; Cvetanović, A.; Švarc-Gajić, J.; Vujić, J. Summer savory extracts prepared by novel extraction methods resulted in enhanced biological activity. Ind. Crops Prod. 2017, 109, 875–881. [Google Scholar]
  37. Nickavar, B.; Mojab, F.; Dolat-Abadi, R. Analysis of the essential oils of two Thymus species from Iran. Food Chem. 2005, 90, 609–611. [Google Scholar] [CrossRef]
  38. Bendif, H.; Adouni, K.; Miara, M.D.; Baranauskienė, R.; Kraujalis, P.; Venskutonis, P.R.; Nabavi, S.M.; Maggi, F. Essential oils (EOs), pressurized liquid extracts (PLE) and carbon dioxide supercritical fluid extracts (SFE-CO2) from Algerian Thymus munbyanus as valuable sources of antioxidants to be used on an industrial level. Food Chem. 2018, 260, 289–298. [Google Scholar] [CrossRef]
  39. Armenta, S.; Garrigues, S.; de la Guardia, M. The role of green extraction techniques in green analytical chemistry. TrAC Trends Anal. Chem. 2015, 71, 2–8. [Google Scholar] [CrossRef]
  40. Armenta, S.; de la Guardia, M.; Namiesnik, J. Green microextraction. In Analytical Microextraction Techniques; Valcarcel, M., Ed.; Bentham Science Publishers: Sharjah, UAE, 2017; pp. 3–27. [Google Scholar]
  41. Armenta, S.; Esteve-Turrillas, F.A.; Garrigues, S.; de la Guardia, M. Green Analytical Chemistry: The Role of Green Extraction Techniques. In Green Extraction Techniques Principles, Advances and Applications; Ibáñez, E., Cifuente, A., Eds.; Elsevier: Amsterdam, The Netherlands, 2017; Volume 76, pp. 1–25. [Google Scholar]
  42. Bravo, J.; Monente, C.; Juániz, I.; De Peña, M.P.; Cid, C. Influence of extraction process on antioxidant capacity of spent coffee. Food Res. Int. 2013, 50, 610–616. [Google Scholar] [CrossRef]
  43. Espino, M.; de los Ángeles Fernández, M.; Gomez, F.J.V.; Silva, M.F. Natural designer solvents for greening analytical chemistry. TrAC Trends Anal. Chem. 2016, 76, 126–136. [Google Scholar] [CrossRef]
  44. Espino, M.; de los Ángeles Fernández, M.; Gomez, F.J.V.; Boiteux, J.; Silva, M.F. Green analytical chemistry metrics: Towards a sustainable phenolics extraction from medicinal plants. Microchem. J. 2018, 141, 438–443. [Google Scholar] [CrossRef]
  45. Khaw, K.Y.; Parat, M.O.; Shaw, P.N.; Falconer, J.R. Solvent supercritical fluid technologies to extract bioactive compounds from natural sources: A review. Molecules 2017, 22, 1186. [Google Scholar] [CrossRef]
  46. Lefebvre, T.; Destandau, E.; Lesellier, E. Selective extraction of bioactive compounds from plants using recent extraction techniques: A review. J. Chromatogr. A 2021, 1635, 461770. [Google Scholar] [CrossRef] [PubMed]
  47. Latif, S.; Anwar, F. Physicochemical studies of hemp (Cannabis sativa) seed oil using enzyme-assisted cold-pressing. Eur. J. Lipid Sci. Technol. 2009, 111, 1042–1048. [Google Scholar] [CrossRef]
  48. Cvetanović, A.; Švarc-Gajić, J.; Mašković, P.; Savić, S.; Nikolić, L.J. Antioxidant and biological activity of chamomile extracts obtained by different techniques: Perspective of using superheated water for isolation of biologically active compounds. Ind. Crops Prod. 2015, 65, 582–591. [Google Scholar]
  49. Belwal, T.; Chemat, F.; Venskutonis, P.R.; Cravotto, G.; Jaiswal, D.K.; Bhatt, I.D.; Devkota, H.P.; Luo, Z. Recent advances in scaling-up of non-conventional extraction techniques: Learning from successes and failures. TrAC Trend Anal. Chem. 2020, 127, 115895. [Google Scholar] [CrossRef]
  50. Kotnik, P.; Skerget, M.; Knez, Z. Supercritical fluid extraction of chamomile flower heads: Comparison with conventional extraction, kinetics and scale-up. J. Supercrit. Fluids 2017, 43, 192–198. [Google Scholar] [CrossRef]
  51. Meullemiestre, A.; Petitcolas, E.; Maache-Rezzoug, Z.; Chemat Rezzoug, S.A. Impact of ultrasound on solid–liquid extraction of phenolic compounds from maritime pine sawdust waste. Kinetics, optimization and large-scale experiments. Ultrason. Sonochem. 2016, 28, 230–239. [Google Scholar] [CrossRef] [PubMed]
  52. Fierascu, R.C.; Sieniawska, E.; Ortan, A.; Fierascu, I.; Xiao, J. Fruits by-products—A source of valuable active principles. A short review. Front. Bioeng. Biotechnol. 2020, 8, 319. [Google Scholar] [CrossRef]
  53. Ferreira, L.F.; Minuzzi, N.M.; Rodrigues, R.F.; Pauletto, R.; Rodrigues, E.; Emanuelli, T.; Bochi, V.C. Citric acid water-based solution for blueberry bagasse anthocyanins recovery: Optimization and comparisons with microwave-assisted extraction (MAE). LWT 2020, 133, 110064. [Google Scholar] [CrossRef]
  54. Yang, Y.; Kayan, B.; Bozer, N.; Pate, B.; Baker, C.; Gizir, A.M. Terpene degradation and extraction from basil and oregano leaves using subcritical water. J. Chromatogr. A 2007, 1152, 262–267. [Google Scholar] [CrossRef] [PubMed]
  55. Rodrigues, L.G.G.; Mazzutti, S.; Vitali, L.; Micke, G.A.; Ferreira, S.R.S. Recovery of bioactive phenolic compounds from papaya seeds agroindustrial residue using subcritical water extraction. Biocatal. Agric. Biotechnol. 2019, 22, 101367. [Google Scholar] [CrossRef]
  56. Majeed, M.; Hussain, A.I.; Chatha, S.A.; Khosa, M.K.; Kamal, G.M.; Kamal, M.A.; Zhang, X.; Liu, M. Optimization protocol for the extraction of antioxidant components from Origanum vulgare leaves using response surface methodology. Saudi J. Biol. Sci. 2016, 23, 389–396. [Google Scholar] [CrossRef] [PubMed]
  57. Aziz, N.A.A.; Hasham, R.; Sarmidi, M.R.; Suhaimi, S.H.; Idris, M.K.H. A review on extraction techniques and therapeutic value of polar bioactives from Asian medicinal herbs: Case study on Orthosiphon aristatus, Eurycoma longifolia and Andrographis paniculate. Saudi Pharm. J. 2021. [Google Scholar] [CrossRef]
  58. Švarc-Gajić, J.; Stojanović, Z.; Carretero, A.S.; Román, D.A.; Borrás, I.; Vasiljević, I. Development of a microwave-assisted extraction for the analysis of phenolic compounds from Rosmarinus officinalis. J. Food Eng. 2013, 119, 525–532. [Google Scholar] [CrossRef]
  59. Thuy Pham, T.P.; Cho, C.W.; Yun, Y.S. Environmental fate and toxicity of ionic liquids: A review. Water Res. 2010, 44, 352–372. [Google Scholar] [CrossRef] [PubMed]
  60. Zhang, L.; Geng, Y.; Duan, W.; Wang, D.; Fu, M.; Wang, X. Ionic liquid-based ultrasoundassisted extraction of fangchinoline and tetrandrine from Stephaniae tetrandrae. J. Sep. Sci. 2009, 32, 3550–3554. [Google Scholar] [CrossRef] [PubMed]
  61. Cao, X.; Ye, X.; Lu, Y.; Yu, Y.; Mo, W. Ionic liquid-based ultrasonic-assisted extraction of piperine from white pepper. Anal. Chim. Acta 2009, 640, 47–51. [Google Scholar] [CrossRef]
  62. Mbous, Y.P.; Hayyan, M.; Hayyan, A.; Wong, W.F.; Hashim, M.A.; Looi, C.Y. Applications of deep eutectic solvents in biotechnology and bioengineering—Promises and challenges. Biotechnol. Adv. 2017, 35, 105–134. [Google Scholar] [CrossRef]
  63. Chemat, F.; Vian, M.A.; Ravi, H.K.; Khadhraoui, B.; Hilali, S.; Perino, S.; Tixier, A.S.F. Review of alternative solvents for green extraction of food and natural products: Panorama, principles, applications and prospects. Molecules 2019, 24, 3007. [Google Scholar] [CrossRef]
  64. Fierascu, R.C.; Fierascu, I.; Avramescu, S.M.; Sieniawska, E. Recovery of natural antioxidants from agro-industrial side streams through advanced extraction techniques. Molecules 2019, 24, 4212. [Google Scholar] [CrossRef] [PubMed]
  65. Skarpalezos, D.; Detsi, A. Deep eutectic solvents as extraction media for valuable flavonoids from natural sources. Appl. Sci. 2019, 9, 4169. [Google Scholar] [CrossRef]
  66. Pan, G.; Yu, G.; Zhu, C.; Qiao, J. Optimization of ultrasound-assisted extraction (UAE) of flavonoids compounds (FC) from hawthorn seed (HS). Ultrason. Sonochem. 2012, 19, 486–490. [Google Scholar] [CrossRef] [PubMed]
  67. Sarfarazi, M.; Jafari, S.M.; Rajabzadeh, G.; Feizi, J. Development of an environmentally-friendly solvent-free extraction of saffron bioactives using subcritical water. LWT 2019, 114, 108428. [Google Scholar] [CrossRef]
  68. Fan, R.; Xiang, J.; Li, N.; Jiang, X.; Gao, Y. Impact of extraction parameters on chemical composition and antioxidant activity of bioactive compounds from Chinese licorice (Glycyrrhiza uralensis Fisch.) by subcritical water. Sep. Sci. Technol. 2016, 51, 609–621. [Google Scholar] [CrossRef]
  69. Munir, M.T.; Kheirkhah, H.; Baroutian, S.; Quek, S.Y.; Young, B.R. Subcritical water extraction of bioactive compounds from waste onion skin. J. Clean. Prod. 2018, 183, 487–494. [Google Scholar] [CrossRef]
  70. Amza, T.; Balla, A.; Tounkara, F.; Man, L.; Zhou, H.M. Effect of hydrolysis time on nutritional, functional and antioxidant properties of protein hydrolysates prepared from gingerbread plum (Neocarya macrophylla) seeds. Int. Food Res. J. 2013, 20, 2081–2090. [Google Scholar]
  71. Skoula, M.; Harborne, J.B. The taxonomy and chemistry of Origanum. In Oregano: The genera Origanum and Lippia; Kintzios, S.E., Ed.; Taylor & Francis: London, UK, 2002; pp. 67–108. [Google Scholar]
  72. Marrelli, M.; Statti, G.A.; Conforti, F. Origanum spp.: An update of their chemical and biological profiles. Phytochem. Rev. 2018, 17, 873–888. [Google Scholar] [CrossRef]
  73. Postu, P.A.; Gorgan, D.L.; Cioanca, O.; Russ, M.; Mikkat, S.; Glocker, M.O.; Hritcu, L. Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study. Antioxidants 2020, 9, 919. [Google Scholar] [CrossRef]
  74. Alonazi, M.A.; Jemel, I.; Moubayed, N.; Alwhibi, M.; El-Sayed, N.N.E.; Bacha, A.B. Evaluation of the in vitro anti-inflammatory and cytotoxic potential of ethanolic and aqueous extracts of Origanum syriacum and Salvia lanigera leaves. Environ. Sci. Pollut. Res. 2021. [Google Scholar] [CrossRef]
  75. Han, F.; Ma, G.; Yang, M.; Yan, L.; Ziong, W.; Shu, J.; Zhao, Z.; Xu, H. Chemical composition and antioxidant activities of essential oils from different parts of the oregano. J. Zhejiang Univ. Sci. B 2017, 18, 79–84. [Google Scholar] [CrossRef] [PubMed]
  76. Khan, A.R.; Nadeem, M.; Bhutto, M.A.; Yu, F.; Xie, X.; El-Hamshary, H.; El-Faham, A.; Ibrahim, U.A.; Mo, X. Physico-chemical and biological evaluation of PLCL/SF nanofibers loaded with oregano essential oil. Pharmaceutics 2019, 11, 386. [Google Scholar] [CrossRef] [PubMed]
  77. Gutiérrez-Grijalva, E.P.; Antunes-Ricardo, M.; Acosta-Estrada, B.A.; Gutiérrez-Uribe, J.A.; Heredia, J.B. Cellular antioxidant activity and in vitro inhibition of α-glucosidase, α-amylase and pancreatic lipase of oregano polyphenols under simulated gastrointestinal digestion. Food Res. Int. 2019, 116, 676–686. [Google Scholar] [CrossRef] [PubMed]
  78. Lee, J.Y.; Garcia, C.V.; Shin, G.H.; Kim, J.T. Antibacterial and antioxidant properties of hydroxypropyl methylcellulose-based active composite films incorporating oregano essential oil nanoemulsions. LWT 2019, 106, 164–171. [Google Scholar] [CrossRef]
  79. Dutra, T.V.; Castro, J.C.; Menezes, J.L.; Ramos, T.R.; Prado, I.N.; Machinski, M.; Mikcha, J.M.G.; de Abreu Filho, B.A. Bioactivity of oregano (Origanum vulgare) essential oil against Alicyclobacillus spp. Ind. Crops Prod. 2019, 129, 345–349. [Google Scholar] [CrossRef]
  80. Khan, A.R.; Huang, K.; Jinzhong, Z.; Zhu, T.; Morsi, Y.; Aldalbahi, A.; El-Newehy, M.; Yan, X.; Mo, X. PLCL/Silk fibroin based antibacterial nano wound dressing encapsulating oregano essential oil: Fabrication, characterization and biological evaluation. Colloid Surf. B Biointerfaces 2020, 196, 111352. [Google Scholar] [CrossRef]
  81. Avola, R.; Granata, G.; Geraci, C.; Napoli, E.; Graziano, A.C.E.; Cardile, V. Oregano (Origanum vulgare L.) essential oil provides anti-inflammatory activity and facilitates wound healing in a human keratinocytes cell model. Food Chem. Toxicol. 2020, 144, 111586. [Google Scholar] [CrossRef]
  82. Fernández-Marín, R.; Labidi, J.; Andrés, M.A.; Fernandes, S.C.M. Using α-chitin nanocrystals to improve the final properties of poly (vinyl alcohol) films with Origanum vulgare essential oil. Polym. Degrad. Stab. 2020, 179, 109227. [Google Scholar] [CrossRef]
  83. Salehi, B.; Ata, A.V.; Anil Kumar, N.; Sharopov, F.; Ramírez-Alarcón, K.; Ruiz-Ortega, A.; Abdulmajid Ayatollahi, S.; Valere Tsouh Fokou, P.; Kobarfard, F.; Amiruddin Zakaria, Z.; et al. Antidiabetic potential of medicinal plants and their active components. Biomolecules 2019, 9, 551. [Google Scholar] [CrossRef]
  84. Radünz, M.; Camargo, T.M.; dos Santos Hackbart, H.C.; Alves, P.I.C.; Radünz, A.L.; Gandra, E.A.; Zavareze, E.R. Chemical composition and in vitro antioxidant and antihyperglycemic activities of clove, thyme, oregano, and sweet orange essential oils. LWT 2021, 138, 110632. [Google Scholar] [CrossRef]
  85. Ayari, S.; Shankar, S.; Follett, P.; Hossain, F.; Lacroix, M. Potential synergistic antimicrobial efficiency of binary combinations of essential oils against Bacillus cereus and Paenibacillus amylolyticus-Part A. Microb. Pathog. 2020, 141, 104008. [Google Scholar] [CrossRef] [PubMed]
  86. Begum, T.; Follett, P.A.; Hossain, F.; Christopher, L.; Salmieri, S.; Lacroix, M. Microbicidal effectiveness of irradiation from Gamma and X-ray sources at different dose rates against the foodborne illness pathogens Escherichia coli, Salmonella Typhimurium and Listeria monocytogenes in rice. LWT 2020, 132, 109841. [Google Scholar] [CrossRef]
  87. Huang, W.; Tao, F.; Li, F.; Mortimer, M.; Guo, L.H. Antibacterial nanomaterials for environmental and consumer product applications. NanoImpact 2020, 20, 100268. [Google Scholar] [CrossRef]
  88. Augustine, R.; Hasan, A. Emerging applications of biocompatible phytosynthesized metal/metal oxide nanoparticles in healthcare. J. Drug Deliv. Sci. Technol. 2020, 56, 101516. [Google Scholar] [CrossRef]
  89. Beikzadeh, S.; Khezerlou, A.; Jafari, S.M.; Pilevar, Z.; Mortazavian, A.M. Seed mucilages as the functional ingredients for biodegradable films and edible coatings in the food industry. Adv. Colloid Interface Sci. 2020, 280, 102164. [Google Scholar] [CrossRef]
  90. Nabavi, S.M.; Marchese, A.; Izadi, M.; Curti, V.; Daglia, M.; Nabavi, S.F. Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmacy. Food Chem. 2015, 173, 339–347. [Google Scholar] [CrossRef] [PubMed]
  91. Salehi, B.; Abu-Darwish, M.S.; Tarawneh, A.H.; Cabral, C.; Gadetskaya, A.V.; Salgueiro, L.; Hosseinabadi, T.; Rajabi, S.; Chanda, W.; Sharifi-Rad, M.; et al. Thymus spp. plants-Food applications and phytopharmacy properties. Trends Food Sci. Technol. 2019, 85, 287–306. [Google Scholar] [CrossRef]
  92. Miguel, G.; Simoes, M.; Figueiredo, A.; Barroso, J.; Pedro, L.; Carvalho, L. Composition and antioxidant activities of the essential oils of Thymus caespititius, Hymus camphoratus and Thymus mastichina. Food Chem. 2004, 86, 183–188. [Google Scholar] [CrossRef]
  93. Giordani, R.; Hadef, Y.; Kaloustian, J. Compositions and antifungal activities of essential oils of some Algerian aromatic plants. Fitoterapia 2008, 79, 199–203. [Google Scholar] [CrossRef]
  94. Pavel, M.; Ristić, M.; Stević, T. Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity. J. Serb. Chem. Soc. 2010, 75, 27–34. [Google Scholar] [CrossRef]
  95. Muslim, S.N.; Hussin, Z.S. Chemical compounds and synergistic antifungal properties of Thymus kotschanus essential oil plus ketoconazole against Candida spp. Gene Rep. 2020, 21, 100916. [Google Scholar] [CrossRef]
  96. Najafloo, R.; Behyari, M.; Imani, R.; Nour, S. A mini-review of Thymol incorporated materials: Applications in antibacterial wound dressing. J. Drug Deliv. Sci. Technol. 2020, 60, 101904. [Google Scholar] [CrossRef]
  97. Guesmi, F.; Khantouche, L.; Mehrez, A.; Bellamine, H.; Landoulsi, A. Histopathological and biochemical effects of thyme essential oil on H2O2 stress in heart tissues. Heart Lung Circ. 2020, 29, 308–314. [Google Scholar] [CrossRef] [PubMed]
  98. Abdelli, I.; Hassani, F.; Brikci, S.B.; Ghalem, S. In silico study the inhibition of angiotensin converting enzyme 2 receptor of COVID-19 by Ammoides verticillata components harvested from Western Algeria. J. Biomol. Struct. Dyn. 2020. [Google Scholar] [CrossRef] [PubMed]
  99. Yepes-Pérez, A.F.; Herrera-Calderon, O.; Sánchez-Aparicio, J.E.; Tiessler-Sala, L.; Maréchal, J.D.; Cardona, G.W. Investigating potential inhibitory effect of Uncaria tomentosa (Cat’s Claw) against the main protease 3CLpro of SARS-CoV-2 by molecular modeling. Evid. Based Complement. Altern. Med. 2020, 2020, 4932572. [Google Scholar]
  100. Asgarpanah, J.; Oveyli, E.; Alidoust, S. Volatile components of the endemic species Salvia sharifii Rech f. & Esfand. J. Essent. Oil Bear. Plants 2017, 20, 578–582. [Google Scholar]
  101. Wei, T.; Gao, Y.; Deng, K.; Zhang, L.; Yang, M.; Liu, X.; Qi, C.; Wang, C.; Song, W.; Zhang, Y.; et al. Enhancement of tanshinone production in Salvia miltiorrhiza hairy root cultures by metabolic engineering. Plant Methods 2019, 15, 53. [Google Scholar] [CrossRef]
  102. Lin, Y.S.; Peng, W.H.; Shih, M.F.; Cherng, J.Y. Anxiolytic effect of an extract of Salvia miltiorrhiza Bunge (Danshen) in mice. J. Ethnopharmacol. 2021, 264, 113285. [Google Scholar] [CrossRef]
  103. Orgah, J.O.; Hea, S.; Wang, Y.; Jiang, M.; Wang, Y.; Orgah, E.A.; Duand, Y.; Zhao, B.; Zhang, B.; Hand, J.; et al. Pharmacological potential of the combination of Salvia miltiorrhiza (Danshen) and Carthamus tinctorius (Honghua) for diabetes mellitus and its cardiovascular complications. Pharmacol. Res. 2020, 153, 104654. [Google Scholar] [CrossRef]
  104. Arras, G.; Piga, A.; Agabbio, M. Effect of TBZ, acetaldehyde, citral and Thymus capitatus essential oil on “Minneola” tangelo fruit decay. Proc. Int. Soc. Citric. 1996, 1, 406–409. [Google Scholar]
  105. Martínez, K.; Ortiz, M.; Albis, A.; Castañeda, C.G.G.; Valencia, M.E.; Grande Tovar, C.D. The effect of edible chitosan coatings incorporated with Thymus capitatus essential oil on the shelf-life of strawberry (Fragaria x ananassa) during cold storage. Biomolecules 2018, 8, 155. [Google Scholar] [CrossRef] [PubMed]
  106. Jemaa, M.B.; Falleh, H.; Serairi, R.; Neves, M.A.; Snoussi, M.; Isoda, H.; Nakajima, M.; Ksouri, R. Nanoencapsulated Thymus capitatus essential oil as natural preservative. Innov. Food Sci. Emerg. Technol. 2018, 45, 92–97. [Google Scholar] [CrossRef]
  107. Arabpoor, B.; Yousefi, S.; Weisany, W.; Ghasemlou, M. Multifunctional coating composed of Eryngium campestre L. essential oil encapsulated in nano-chitosan to prolong the shelf-life of fresh cherry fruits. Food Hydrocoll. 2021, 111, 106394. [Google Scholar] [CrossRef]
  108. Mehdizadeh, T.; Tajik, H.; Langroodi, A.M.; Molaei, R.; Mahmoudian, A. Chitosan-starch film containing pomegranate peel extract and Thymus kotschyanus essential oil can prolong the shelf life of beef. Meat Sci. 2020, 163, 108073. [Google Scholar] [CrossRef] [PubMed]
  109. Javaherzadeh, R.; Tabatabaee Bafroee, A.S.; Kanjari, A. Preservation effect of Polylophium involucratum essential oil incorporated poly lactic acid/ nanochitosan composite film on shelf life and sensory properties of chicken fillets at refrigeration temperature. LWT 2020, 118, 108783. [Google Scholar] [CrossRef]
  110. Rehman, A.; Jafari, S.M.; Aadil, R.M.; Assadpour, E.; Randhawa, M.A.; Mahmood, S. Development of active food packaging via incorporation of biopolymeric nanocarriers containing essential oils. Trends Food Sci. Technol. 2020, 101, 106–121. [Google Scholar] [CrossRef]
  111. Hosseini, H.; Hamgini, E.Y.; Jafari, S.M.; Bolourian, S. Improving the oxidative stability of sunflower seed kernels by edible biopolymeric coatings loaded with rosemary extract. J. Stored Prod. Res. 2020, 89, 101729. [Google Scholar] [CrossRef]
  112. Choulitoudi, E.; Ganiari, S.; Tsironi, T.; Ntzimani, A.; Tsimogiannis, D.; Taoukis, P.; Oreopoulou, V. Edible coating enriched with rosemary extracts to enhance oxidative and microbial stability of smoked eel fillets. Food Packag. Shelf Life 2017, 12, 107–113. [Google Scholar] [CrossRef]
  113. Pavela, R.; Benelli, G.; Canale, A.; Maggi, F.; Mártonfi, P. Exploring essential oils of Slovak medicinal plants for insecticidal activity: The case of Thymus alternans and Teucrium montanum subsp. Jailae. Food Chem. Toxicol. 2020, 138, 111203. [Google Scholar] [CrossRef]
  114. Bozkurt, M.; Tüzün, A.E. Application of aromatic plants and their extracts in diets of turkeys, In Feed Additives. Aromatic Plants and Herbs in Animal Nutrition and Health, 1st ed.; Florou-Paneri, P., Christaki, E., Giannenas, I., Eds.; Academic Press: London, UK, 2020; pp. 205–226. [Google Scholar]
  115. Saroj, A.; Oriyomi, O.V.; Nayak, A.K.; Haider, Z. Phytochemicals of plant-derived essential oils: A novel green approach against pests. In Natural Remedies for Pest, Disease and Weed Control; Egbuna, C., Sawicka, B., Eds.; Academic Press: London, UK, 2020; pp. 65–79. [Google Scholar]
  116. de Paris, M.; Stivanin, S.C.B.; Klein, C.P.; Vizzotto, E.F.; Passos, L.T.; Angelo, I.D.V.; Zanela, M.B.; Stone, V.; Matté, C.; Heisler, G.; et al. Calves fed with milk from cows receiving plant extracts improved redox status. Livest. Sci. 2020, 242, 104272. [Google Scholar] [CrossRef]
  117. Harhaun, R.; Kunik, O.; Saribekova, D.; Lazzara, G. Biologically active properties of plant extracts in cosmetic emulsions. Microchem. J. 2020, 154, 104543. [Google Scholar] [CrossRef]
  118. Bom, S.; Jorge, J.; Ribeiro, H.M.; Marto, J. A step forward on sustainability in the cosmetics industry: A review. J. Clean. Prod. 2019, 225, 270–290. [Google Scholar] [CrossRef]
  119. Chou, S.T.; Chang, W.L.; Chang, C.T.; Hsu, S.L.; Lin, Y.C.; Shih, Y. Cinnamomum cassia essential oil inhibits α-MSH-induced melanin production and oxidative stress in murine B16 melanoma cells. Int. J. Mol. Sci. 2013, 14, 19186–19201. [Google Scholar] [CrossRef] [PubMed]
  120. Aumeeruddy-Elalfi, Z.; Lall, N.; Fibrich, B.; Van Staden, A.B.; Hosenally, M.; Mahomoodally, M.F. Selected essential oils inhibit key physiological enzymes and possess intracellular and extracellular antimelanogenic properties in vitro. J. Food Drug Anal. 2018, 26, 232–243. [Google Scholar] [CrossRef] [PubMed]
  121. Maria, M.F.F.; Ikhmal, W.M.K.W.M.; Amirah, M.N.N.S.; Manja, S.M.; Syaizwadi, S.M.; Chan, K.S.; Sabri, M.G.M.; Adnan, A. Green approach in anti-corrosion coating by using Andrographis paniculata leaves extract as additives of stainless steel 316L in seawater. Int. J. Corros. Scale Inhib. 2019, 8, 644–658. [Google Scholar]
  122. Ong, G.; Kasi, R.; Subramaniam, R. A review on plant extracts as natural additives in coating applications. Prog. Org. Coat. 2021, 151, 106091. [Google Scholar] [CrossRef]
  123. Brobbey, K.J.; Saarinen, J.; Alakomi, H.; Yang, B.; Toivakka, M. Efficacy of natural plant extracts in antimicrobial packaging systems. J. Appl. Packag. Res. 2017, 9, 60–71. [Google Scholar]
  124. Shlar, I.; Droby, S.; Choudhary, R.; Rodov, V. The mode of antimicrobial action of curcumin depends on the delivery system: Monolithic nanoparticles: Vs. Supramolecular inclusion complex. RSC Adv. 2017, 7, 42559–42569. [Google Scholar] [CrossRef]
  125. Agostini, V.O.; Macedo, A.J.; Muxagata, E.; da Silva, M.V.; Pinho, G.L.L. Non-toxic antifouling potential of Caatinga plant extracts: Effective inhibition of marine initial biofouling. Hydrobiologia 2020, 847, 45–60. [Google Scholar] [CrossRef]
  126. Tomak, E.D.; Yazici, O.A.; Parmak, E.D.S.; Gonultas, O. Influence of tannin containing coatings on weathering resistance of wood: Combination with zinc and cerium oxide nanoparticles. Polym. Degrad. Stab. 2018, 152, 289–296. [Google Scholar] [CrossRef]
  127. Peng, Y.; Wang, Y.; Chen, P.; Wang, W.; Cao, J. Enhancing weathering resistance of wood by using bark extractives as natural photostabilizers in polyurethane-acrylate coating. Prog. Org. Coat. 2020, 145, 105665. [Google Scholar] [CrossRef]
  128. Iamareerat, B.; Singh, M.; Sadiq, M.B.; Anal, A.K. Reinforced cassava starch based edible film incorporated with essential oil and sodium bentonite nanoclay as food packaging material. J. Food Sci. Technol. 2018, 55, 1953–1959. [Google Scholar] [CrossRef] [PubMed]
  129. Han, C.; Wang, X.; Zhang, D.; Wei, Y.; Cui, Y.; Shia, W.; Bao, Y. Synergistic use of florfenicol and Salvia miltiorrhiza polysaccharide can enhance immune responses in broilers. Ecotoxicol. Environ. Saf. 2021, 210, 111825. [Google Scholar] [CrossRef] [PubMed]
  130. Jokanović, M.; Snežan, M.; Vladimir Tomović, S.; Pavlić, B.; Šojić, B.; Zeković, Z.; Peulić, T.; Ikonić, P. Essential oil and supercritical extracts of winter savory (Satureja montana L.) as antioxidants in precooked pork chops during chilled storage. LWT 2020, 134, 110260. [Google Scholar] [CrossRef]
  131. Šojić, B.; Tomović, V.; Kocić-Tanackov, S.; Bursać Kovačević, D.; Putnik, P.; Mrkonjić, Z.; Đurović, S.; Jokanović, M.; Snežan, M.; Branimir Pavlić, S. Supercritical extracts of wild thyme (Thymus serpyllum L.) by-product as natural antioxidants in ground pork patties. LWT 2020, 130, 109661. [Google Scholar] [CrossRef]
  132. Badawy, M.E.I.; Lotfy, T.M.R.; Shawir, S.M.S. Facile synthesis and characterizations of antibacterial and antioxidant of chitosan monoterpene nanoparticles and their applications in preserving minced meat. Int. J. Biol. Macromol. 2020, 156, 127–136. [Google Scholar] [CrossRef] [PubMed]
  133. Fierascu, I.; Fierascu, I.C.; Brazdis, R.I.; Baroi, A.M.; Fistos, T.; Fierascu, R.C. Phytosynthesized metallic nanoparticles—between nanomedicine and toxicology. A brief review of 2019′s findings. Materials 2020, 13, 574. [Google Scholar] [CrossRef]
  134. Sharma, V.; Kaushik, S.; Pandit, P.; Dhull, D.; Yadav, J.P.; Kaushik, S. Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungunya virus. Appl. Microbiol. Biotechnol. 2019, 103, 881–891. [Google Scholar] [CrossRef]
  135. Sundararajan, B.; Kumari, B.R. Novel synthesis of gold nanoparticles using Artemisia vulgaris L. leaf extract and their efficacy of larvicidal activity against dengue fever vector Aedes aegypti L. J. Trace Elem. Med. Biol. 2017, 43, 187–196. [Google Scholar] [CrossRef]
  136. Chandra, H.; Patel, D.; Kumari, P.; Jangwan, J.S.; Yadav, S. Phytomediated synthesis of zinc oxide nanoparticle of Berberis aristata: Characterisation, antioxidant activity and antibacterial activity with special reference to urinary tract infection. Mater. Sci. Eng. C 2019, 102, 212–220. [Google Scholar] [CrossRef]
  137. Fierascu, R.C.; Fierascu, I.; Lungulescu, E.M.; Nicula, N.; Somoghi, R.; Diţu, L.M.; Ungureanu, C.; Sutan, A.N.; Drăghiceanu, O.A.; Paunescu, A.; et al. Phytosynthesis and radiation-assisted methods for obtaining metal nanoparticles. J. Mater. Sci. 2020, 55, 1915–1932. [Google Scholar] [CrossRef]
  138. Wu, H.; Yin, J.J.; Wamer, W.G.; Zeng, M.; Lo, Y.M. Reactive oxygen species-related activities of nano-iron metal and nano-iron oxides. J. Food Drug Anal. 2014, 22, 86–94. [Google Scholar] [CrossRef] [PubMed]
  139. Opris, R.; Tatomir, C.; Olteanu, D.; Moldovan, R.; Moldovan, B.; David, L.; Nagy, A.; Decea, N.; Kiss, M.L.; Filip, A.G. The effect of Sambucus nigra L. extract and phytosinthesized gold nanoparticles on diabetic rats. Colloids Surf. B Biointerfaces 2017, 150, 192–200. [Google Scholar] [CrossRef] [PubMed]
  140. Kumar, H.; Bhardwaj, K.; Nepovimova, E.; Kuča, K.; Singh Dhanjal, D.; Bhardwaj, S.; Bhatia, S.K.; Verma, R.; Kumar, D. Antioxidant functionalized nanoparticles: A combat against oxidative stress. Nanomaterials 2020, 10, 1334. [Google Scholar] [CrossRef] [PubMed]
  141. Prabhakar, R.; Samadder, S.R. Aquatic and terrestrial weed mediated synthesis of iron nanoparticles for possible application in wastewater remediation. J. Clean. Prod. 2017, 168, 1201–1210. [Google Scholar] [CrossRef]
  142. Yuan, M.; Fu, X.; Yu, J.; Xu, Y.; Huang, J.; Lia, Q.; Sun, D. Green synthesized iron nanoparticles as highly efficient fenton-like catalyst for degradation of dyes. Chemosphere 2020, 261, 127618. [Google Scholar] [CrossRef] [PubMed]
  143. Alabi, M.A.; Muthusamy, A.; Kabekkodu, S.P.; Adebawo, O.O.; Satyamoorthy, K. Anticancer properties of recipes derived from nigeria and african medicinal plants on breast cancer cells in vitro. Sci. Afr. 2020, 8, e00446. [Google Scholar] [CrossRef]
  144. Dzah, C.S.; Duan, Y.; Zhang, H.; Adwo, N.; Boateng, S.; Ma, H. Latest developments in polyphenol recovery and purification from plant by-products: A review. Trends Food Sci. Technol. 2020, 99, 375–388. [Google Scholar] [CrossRef]
  145. Amin, F.U.; Hoshiar, A.K.; Do, T.D.; Noh, Y.; Shah, S.A.; Khan, M.S.; Yoon, J.; Kim, M.O. Osmotin-loaded magnetic nanoparticles with electromagnetic guidance for the treatment of Alzheimer’s disease. Nanoscale 2017, 9, 10619–10632. [Google Scholar] [CrossRef]
  146. Bilal, M.; Barani, M.; Sabir, F.; Rahdar, A.; Kyzas, G.Z. Nanomaterials for the treatment and diagnosis of Alzheimer’s disease: An overview. NanoImpact 2020, 20, 100251. [Google Scholar] [CrossRef]
Figure 1. Some of the potential applications of medicinal and aromatic plants.
Figure 1. Some of the potential applications of medicinal and aromatic plants.
Ijms 22 01521 g001
Figure 2. Commonly used solvents in the extraction process.
Figure 2. Commonly used solvents in the extraction process.
Ijms 22 01521 g002
Table 1. Comparation of classical and modern extraction techniques for medicinal and aromatic plants.
Table 1. Comparation of classical and modern extraction techniques for medicinal and aromatic plants.
PlantExtraction MethodExtraction
Extraction YieldReference
sativum Linn.
Microwave-assisted hydro-distillationSolvent: deionized water/diethyl ether 2:1; 100 g vegetal material;
MP = 700 W; t = 30 min;
Diallyl sulfides (mono-, di-, tri-, and tetra-);
Methyl allyl sulfides (di- and tri-);
Vinyl dithiins
Ultrasound-assisted extractionSolvent: diethyl ether (50 mL); F = 35 kHz;
T = 25 °C; t = 30 min.
Lickens–Nickerson apparatusSolvent: water/diethyl ether = 1:10; 100 g. vegetal material; T = −10 °C; t = 2 h. 0.23%
Hippophae rhamnoides L.Solvent-free microwave-assisted extraction400 g vegetal material atmospheric pressure;
P = 400 W;
T = 20–100 °C; t = 15 min.
Polyphenols with an increased yield of recovery for microwave extraction method1147 mg GAE/g (d.w.)[23]
Classical extractionSolvent: methanol 80% (50 mL); 5 g vegetal material;
8000 rpm; t = 5 min.
741.9 mg GAE/g (d.w.)
Matricaria chamomilla L.Subcritical water
Solvent: water (300 mL); 10 g vegetal material; P = 30, 45 and 60 bars;
T = 100 °C; t = 30 min;
Polyphenols127–3226 mg/kg[24]
MacerationSolvent: water (100 mL); 2.5 g vegetal material—oven-dried chamomile at low temperatures (i.e.,
40 °C);
T = 100 °C; t = 120 min.
Polyphenols19.7 ± 0.5 mg/g (d.w.)[25]
Mentha spp.Microwave hydro-
Solvent-free; 500 g vegetal material;
MP 1 W/g; F 2.45 GHz
t = 20 min.
Essential oil0.95%[26]
Soxhlet extractionSolvent: water: ethanol = 3:7 (250 mL); 1.5 g dry plant material;
T = 95 °C
Polyphenols18,381–87,024 mg GAE/kg (d.w.)[27]
Origanum vulgare L., 1753Supercritical
CO2 flow rate = 2.4 kg/h; 0.6 kg of vegetal material: CO2/plant ratio = 20 kg/kg; P = 30 MPa; T = 40 °C.Carnosic acid3.18 ± 0.40%[28]
Hydro-distillation300 g vegetal material;
t = 45 min
Essential oil rich in terpenes0.75% (d.w.)[29]
Rosmarinus officinalis L.MacerationSolvent: dichloromethane/ethanol = 3/1 (15 mL); 1 g vegetal material;
T = 35 °C; t 3 h.
Carnosic acid, rosmarinic acid, carnosol16.82; 0.12; 9.31 mg/g (f.w.)[30]
Supercritical fluid
Solvent-free CO2 extraction; flow rate: 5 g/min; 100 g vegetal material; P = 100–300 bar;
T = 40 °C; t = 3 h.
Carnosic acid, rosmarinic acid, camphor, 1,8-cineole1.0730; 0.1242; 0.44; 0.029% (d.w.)[31]
Microwave-assisted extractionSolvent: ethanol 96 %; 25 g milled leaves;
Liquid/solid ratio =
6/1 (v/w); t = 7 min.
Carnosic acid,
rosmarinic acid
3.3 ± 0.2 % (w/v)
3.1 ± 1.2 % (w/v)
officinalis L.
Supercritical CO2
Solvent-free CO2 extraction; flow rate: 1–3 kg/h; 50 g. of vegetal material;
P = 15 or 20 MPa;
T = 25 °C; t = 90 min.
Terpenes and
phenolic compounds
0.659–5.477 % (w/v)[33]
Hydro distillation (Clevenger-type
Solvent: water (1 L); 100 g vegetal material;
t = 180 min.
Terpenes and phenolic compounds2.0–2.1% (v/w)[34]
MacerationSolvent: ethanol (70%)—25 mL; 5 g of vegetal material;
t = 2 days;
Rosmarinic acid, carnosic acid, carnosol and methyl carnosaten.p.[35]
Satureja hortensis L.MacerationSolvent: ethanol 96% (300 mL); 10 g vegetal material;
T = 22 °C; t = 7 days.
125.34 mg GAE/g[36]
Soxhlet extractionSolvent: ethanol 96% (600 mL); 75 g vegetal material;
t = 8 h.
119.28 mg GAE/g
Microwave extractionSolvent: ethanol 96% (100 mL); 5 g vegetal material;
t = 30 min
147.21 mg GAE/g
daenensis Celak. and Thymus
kotschyanus Boiss. and Hohen
Hydro-distillation (Clevenger-type
50 g vegetal material;
t = 3 h.
methyl carvacrol
Thymus munbyanus Boiss. & Reut., 1852Pressurized liquid
Solvent: acetone; ethanol; water; 20 g. vegetal material; P = 45 MPa;
T = 70 °C; t = 10 min
Oxygenated monoterpenoids; sesquiterpenoids and monoterpenoids21.2 ± 0.6%[38]
Where d.w.—dry weight; F—frequency; f.w.—fresh weight; GAE—gallic acid equivalents; MP—microwave power; n.p. —not provided by the authors; P—pressure; T—temperature; t—time.
Table 2. Some examples of the industrial applications of medicinal and aromatic plants (MAPs).
Table 2. Some examples of the industrial applications of medicinal and aromatic plants (MAPs).
Artemisia absinthium L., Calendula officinalis L., Lavandula vera DC,
Syringa vulgaris L.
Water-soluble vitaminsEmulsionAntioxidant activityCosmetic industry[117]
CinnamonEssential oilStarch based edible filmAntibacterial activity (Escherichia coli, Salmonella typhimurium and Staphylococcus aureus)Natural packaging[128]
Eryngium campestre L.Essential oilChitosan
-Prolonging shelf life of sweet cherries[107]
Polylophium involucratum (Pall.) Boiss.Essential oilPoly lactic acid/ nanochitosan composite filmAntimicrobial activity (Pseudomonas spp.)Prolonging shelf life of chicken fillet[109]
Psiadia terebinthina A.J. ScottEssential oil-Melanin inhibitionCosmetic industry[120]
Salvia miltiorrhiza BungePolysaccharide-Increase the number of leukocytes in bloodIncrease growth performance of broilers[129]
Salvia rosmarinus Spenn.Aqueous extractWhey protein concentrate/carboxymethyl cellulose/glycerol coatings-Coatings for sun flower seeds[111]
Satureja montana L.Essential oil-Protein oxidative
Lipid oxidative
Prolonging shelf life of pre-cooked pork chops[130]
Thymus alternans Klokov and Teucrium montanum subsp. JailaeEssential oil-Insecticide (Musca domestica L., Culex quinquefasciatus Say and Spodoptera littoralis (Boisd.))Natural insecticide[113]
Thymus capitatus (L.) Hoffmanns et LinkEssential oilChitosan coatingsAntibacterial activity (Aerobic mesophylls, molds and yeasts)Prolonged up to 1 day shelf life of strawberries stored under refrigeration conditions (5 ± 0.5 °C)[105]
Thymus capitatus (L.) Hoffmanns et LinkEssential oilNano-emulsionAntibacterial activity (Staphylococcus aureus)Food preservative[106]
Thymus kotschyanus Boiss. & Hohen.Essential oilChitosan–starch composite filmAntibacterial activity (Pseudomonas spp. and Listeria monocytogenes)Prolonging shelf life of beef during storage on a period of 21 days at 4 °C[108]
Thymus serpyllum L.Essential oil-Antimicrobial activity (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Pseudomonas aeruginosa)Ground pork patty[131]
-Limonene, linalool, menthol, and thymolChitosan
Antimicrobial activity (Escherichia coli and Salmonella typhimurium)Preservation of minced meat[132]
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