A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies

3B’s Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal

ICVS/3B’s-PT Government Associate Laboratory, 4805-017 Guimarães, Portugal

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Cell Physiology Research Group, Department of Physiology, University of Extremadura, 10003 Cáceres, Spain

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Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 Cáceres, Spain

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Centro de Investigación en Red en Enfermedades Cardiovasculares (CIBERCV), 28029 Madrid, Spain

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Immunology Unit, Department of Physiology, University of Extremadura, 10003 Cáceres, Spain

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work and should be regarded as co-senior authors.

Academic Editor: Agata Przekora

Int. J. Mol. Sci. 2021, 22(24), 13385; https://doi.org/10.3390/ijms222413385

Received: 19 November 2021 / Revised: 7 December 2021 / Accepted: 8 December 2021 / Published: 13 December 2021

(This article belongs to the Special Issue Interactions of Cells with Biomaterials for Regenerative Medicine 2.0)

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Abstract

Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (p < 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses. View Full-Text

Keywords: surgical mesh; fibrin sealant; coating; fibrin; cell therapy; menstrual blood-derived stromal cells

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MDPI and ACS Style

Marinaro, F.; Silva, J.M.; Barros, A.A.; Aroso, I.M.; Gómez-Blanco, J.C.; Jardin, I.; Lopez, J.J.; Pulido, M.; de Pedro, M.Á.; Reis, R.L.; Sánchez-Margallo, F.M.; Casado, J.G.; López, E. A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies. Int. J. Mol. Sci. 2021, 22, 13385. https://doi.org/10.3390/ijms222413385

AMA Style

Marinaro F, Silva JM, Barros AA, Aroso IM, Gómez-Blanco JC, Jardin I, Lopez JJ, Pulido M, de Pedro MÁ, Reis RL, Sánchez-Margallo FM, Casado JG, López E. A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies. International Journal of Molecular Sciences. 2021; 22(24):13385. https://doi.org/10.3390/ijms222413385

Chicago/Turabian Style

Marinaro, Federica, Joana M. Silva, Alexandre A. Barros, Ivo M. Aroso, Juan C. Gómez-Blanco, Isaac Jardin, Jose J. Lopez, María Pulido, María Á. de Pedro, Rui L. Reis, Francisco M. Sánchez-Margallo, Javier G. Casado, and Esther López. 2021. "A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies" International Journal of Molecular Sciences 22, no. 24: 13385. https://doi.org/10.3390/ijms222413385

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A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies

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