RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons
Abstract
:1. Introduction
2. Results
2.1. Stau2 Binds RNA Structures in the 3′-UTR In Vivo
2.2. A Defined Long-Range RNA Structure Recruits Stau2
2.3. RNA Structure Conveys Stau2-Dependent Dendritic mRNA Localization
2.4. Rgs4 RNA Structure Contributes to Protein Expression
3. Discussion
4. Materials and Methods
4.1. Plasmids
4.2. Neuronal Cell Culture, Treatment, Transduction and Transfection
4.3. Lentivirus Production
4.4. RNA Extraction, cDNA Synthesis and qPCR
4.5. Bioinformatics
4.6. Rgs4 RNA Secondary Structure Prediction
4.7. Luciferase Assay
4.8. Imaging-Based GFP Expression Assay in Primary Neurons
4.9. Imaging and Data Analysis
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fernández-Moya, S.M.; Ehses, J.; Bauer, K.E.; Schieweck, R.; Chakrabarti, A.M.; Lee, F.C.Y.; Illig, C.; Luscombe, N.M.; Harner, M.; Ule, J.; et al. RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons. Int. J. Mol. Sci. 2021, 22, 13021. https://doi.org/10.3390/ijms222313021
Fernández-Moya SM, Ehses J, Bauer KE, Schieweck R, Chakrabarti AM, Lee FCY, Illig C, Luscombe NM, Harner M, Ule J, et al. RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons. International Journal of Molecular Sciences. 2021; 22(23):13021. https://doi.org/10.3390/ijms222313021
Chicago/Turabian StyleFernández-Moya, Sandra M., Janina Ehses, Karl E. Bauer, Rico Schieweck, Anob M. Chakrabarti, Flora C. Y. Lee, Christin Illig, Nicholas M. Luscombe, Max Harner, Jernej Ule, and et al. 2021. "RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons" International Journal of Molecular Sciences 22, no. 23: 13021. https://doi.org/10.3390/ijms222313021