Enhancer RNA Profiling in Smoking and HPV Associated HNSCC Reveals Associations to Key Oncogenes
Abstract
:1. Introduction
2. Results
2.1. TCGA Data Acquisition and Extraction of eRNA Reads
2.2. Differential Expression of eRNA between Cancer and Normal Tissue Samples
2.3. Correlation of eRNA to Survival of Patients
2.4. Correlation of eRNA to Clinical Variables
2.5. Correlation of eRNA to Immune Infiltration
2.6. Correlation of eRNA to Cancer and Immune-Associated Signatures
2.7. Correlation of eRNA to Genomic Alterations
2.8. Differential Expression of eRNA between Cancer and Normal Samples from Secondary Validation Dataset
3. Discussion
4. Materials and Methods
4.1. Data Acquisition from TCGA
4.2. Extraction of eRNA Reads
4.3. Differential Expression of eRNA between Cancer and Normal Tissue Samples
4.4. Correlation of eRNA to Survival of Patients
4.5. Correlation of eRNA to Clinical Variables
4.6. Correlation of eRNA to Immune Infiltration
4.7. Correlation of eRNA to Cancer and Immune-Associated Signatures
4.8. Correlation of eRNA to Genomic Alterations
4.9. Validation of TCGA Results with Secondary Dataset
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shende, N.; Xu, J.; Li, W.T.; Liu, J.; Chakladar, J.; Brumund, K.T.; Ongkeko, W.M. Enhancer RNA Profiling in Smoking and HPV Associated HNSCC Reveals Associations to Key Oncogenes. Int. J. Mol. Sci. 2021, 22, 12546. https://doi.org/10.3390/ijms222212546
Shende N, Xu J, Li WT, Liu J, Chakladar J, Brumund KT, Ongkeko WM. Enhancer RNA Profiling in Smoking and HPV Associated HNSCC Reveals Associations to Key Oncogenes. International Journal of Molecular Sciences. 2021; 22(22):12546. https://doi.org/10.3390/ijms222212546
Chicago/Turabian StyleShende, Neil, Jingyue Xu, Wei Tse Li, Jeffrey Liu, Jaideep Chakladar, Kevin T. Brumund, and Weg M. Ongkeko. 2021. "Enhancer RNA Profiling in Smoking and HPV Associated HNSCC Reveals Associations to Key Oncogenes" International Journal of Molecular Sciences 22, no. 22: 12546. https://doi.org/10.3390/ijms222212546