Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas
Abstract
:1. Introduction
2. Results and Discussion
2.1. Generation of the Syt13-Venus Fusion Mouse Line
2.2. Syt13 Is Highly Expressed in Neuroendocrine Cells
2.3. Syt13 Expression Is Restricted to Enteroendocrine Cells in the Adult Intestine
2.4. Pancreatic Endocrine but Not Exocrine Cells Specifically Express Syt13 Protein
3. Materials and Methods
3.1. Generation of the Targeting Vector
3.2. Embryonic Stem Cell (ESC) Homologous Recombination and Generation of Mouse Line
3.3. Genotyping
3.4. Organ Dissection and Immunostaining
3.5. Neuronal and Glia Cell Isolation
3.6. Crypt Isolation, Culturing and Live Imaging
3.7. Pancreatic Islet and Exocrine Tissue Isolation and FACS Sorting
3.8. RNA Isolation, RT-PCR and qPCR
3.9. ScRNA-Seq Data Sources and Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tarquis-Medina, M.; Scheibner, K.; González-García, I.; Bastidas-Ponce, A.; Sterr, M.; Jaki, J.; Schirge, S.; García-Cáceres, C.; Lickert, H.; Bakhti, M. Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas. Int. J. Mol. Sci. 2021, 22, 12526. https://doi.org/10.3390/ijms222212526
Tarquis-Medina M, Scheibner K, González-García I, Bastidas-Ponce A, Sterr M, Jaki J, Schirge S, García-Cáceres C, Lickert H, Bakhti M. Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas. International Journal of Molecular Sciences. 2021; 22(22):12526. https://doi.org/10.3390/ijms222212526
Chicago/Turabian StyleTarquis-Medina, Marta, Katharina Scheibner, Ismael González-García, Aimée Bastidas-Ponce, Michael Sterr, Jessica Jaki, Silvia Schirge, Cristina García-Cáceres, Heiko Lickert, and Mostafa Bakhti. 2021. "Synaptotagmin-13 Is a Neuroendocrine Marker in Brain, Intestine and Pancreas" International Journal of Molecular Sciences 22, no. 22: 12526. https://doi.org/10.3390/ijms222212526