HBV HBx-Downregulated lncRNA LINC01010 Attenuates Cell Proliferation by Interacting with Vimentin
Abstract
:1. Introduction
2. Results
2.1. HBV HBx-Downregulated lncRNA LINC01010 Is Abnormally Expressed in HCC
2.2. LINC01010 Attenuates the Cell Proliferation, Migration and Invasion
2.3. LINC01010 Interacts with Vimentin
2.4. Overexpression of LINC01010 Inhibits the Network Extension and Assembly of Vimentin
2.5. Knockdown of LINC01010 Promotes the Network Extension and Assembly of Vimentin
3. Discussion
4. Materials and Methods
4.1. Plasmids and Antibodies
4.2. Cell Lines and Cell Culture
4.3. Tumor Samples
4.4. RNA Interference
4.5. Rapid Amplification of cDNA Ends (RACE)
4.6. Luciferase Reporter Assay
4.7. Quantitative RT-PCR (qRT-PCR)
4.8. Immunoblotting
4.9. Cell Growth Assay
4.10. Migration and Invasion Assays
4.11. S1 Pull-Down
4.12. RNA Immunoprecipitation (RIP)
4.13. In Vitro RNA Pull-Down Assay
4.14. Cell Extension Formation and Immunofluorescence Assays
4.15. Fluorescence Recovery after Photobleaching (FRAP)
4.16. Vimentin Solubility Assay
4.17. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gan, L.; Shangguan, Q.; Zhang, F.; Tong, X.; Qi, D.; Zhao, Y.; Ye, X. HBV HBx-Downregulated lncRNA LINC01010 Attenuates Cell Proliferation by Interacting with Vimentin. Int. J. Mol. Sci. 2021, 22, 12497. https://doi.org/10.3390/ijms222212497
Gan L, Shangguan Q, Zhang F, Tong X, Qi D, Zhao Y, Ye X. HBV HBx-Downregulated lncRNA LINC01010 Attenuates Cell Proliferation by Interacting with Vimentin. International Journal of Molecular Sciences. 2021; 22(22):12497. https://doi.org/10.3390/ijms222212497
Chicago/Turabian StyleGan, Lipeng, Qilin Shangguan, Fang Zhang, Xiaomei Tong, Dandan Qi, Yan Zhao, and Xin Ye. 2021. "HBV HBx-Downregulated lncRNA LINC01010 Attenuates Cell Proliferation by Interacting with Vimentin" International Journal of Molecular Sciences 22, no. 22: 12497. https://doi.org/10.3390/ijms222212497