The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast
Abstract
:1. Introduction
2. Results
2.1. The Rpd3 Complex Is Required for Efficient Surface Protein Recycling
2.2. Hypothesis for the Role of the Rpd3-Complex in Recycling
2.3. Downstream Rpd3 Targets Regulate Recycling
2.4. Rpd3 Regulates the Phosphatidylinositol 4-Kinase Pik1
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Yeast Strains and Culture Conditions
4.3. Bacterial Culture
4.4. DNA Manipulations
4.5. Fluorescence Microscopy
4.6. Flow Cytometry FM4-64 Recycling Assay
4.7. Immunoblotting
4.8. Bioinformatics and Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Amoiradaki, K.; Bunting, K.R.; Paine, K.M.; Ayre, J.E.; Hogg, K.; Laidlaw, K.M.E.; MacDonald, C. The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast. Int. J. Mol. Sci. 2021, 22, 12477. https://doi.org/10.3390/ijms222212477
Amoiradaki K, Bunting KR, Paine KM, Ayre JE, Hogg K, Laidlaw KME, MacDonald C. The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast. International Journal of Molecular Sciences. 2021; 22(22):12477. https://doi.org/10.3390/ijms222212477
Chicago/Turabian StyleAmoiradaki, Konstantina, Kate R. Bunting, Katherine M. Paine, Josephine E. Ayre, Karen Hogg, Kamilla M. E. Laidlaw, and Chris MacDonald. 2021. "The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast" International Journal of Molecular Sciences 22, no. 22: 12477. https://doi.org/10.3390/ijms222212477