Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics
Abstract
:1. Introduction
2. Results
2.1. Characteristics of β-TCP
2.2. SIPS Cells in Bone Defects after β-TCP Implantation
2.3. Administration of Senolytics after β-TCP Implantation
2.4. Histomorphometric Analysis of the Bone Formation and β-TCP Resorption
2.5. Osteoclastogenesis in Bone Defects
2.6. Reactive Oxidative Stress in Bone Defects
3. Discussion
4. Materials and Methods
4.1. β-TCP Granules
4.2. Characterization of β-TCP Granules
4.3. The Preparation of the Senolytics Using Dasatinib and Quercetin
4.4. Animal Experiment
4.5. The Analysis of Bone Histomorphometry Utilizing Microcomputed Tomography
4.6. Histological Staining
4.7. Immunofluorescence
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, X.; Honda, Y.; Zhao, J.; Morikuni, H.; Nishiura, A.; Hashimoto, Y.; Matsumoto, N. Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics. Int. J. Mol. Sci. 2021, 22, 12415. https://doi.org/10.3390/ijms222212415
Wang X, Honda Y, Zhao J, Morikuni H, Nishiura A, Hashimoto Y, Matsumoto N. Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics. International Journal of Molecular Sciences. 2021; 22(22):12415. https://doi.org/10.3390/ijms222212415
Chicago/Turabian StyleWang, Xinchen, Yoshitomo Honda, Jianxin Zhao, Hidetoshi Morikuni, Aki Nishiura, Yoshiya Hashimoto, and Naoyuki Matsumoto. 2021. "Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics" International Journal of Molecular Sciences 22, no. 22: 12415. https://doi.org/10.3390/ijms222212415