Scleroderma-like Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis
Abstract
:1. Introduction
2. Results
2.1. Verification of the Effective Development of the Bleomycin-Induced Dermal Fibrosis Mouse Model
2.2. Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Dermis of Bleomycin-Treated Mice
3. Discussion
4. Materials and Methods
4.1. Mouse Model of Bleomycin-Induced Dermal Fibrosis
4.2. Histochemical Analysis and Evaluation of Dermal Thickness
4.3. Hydroxyproline Assay
4.4. Transmission Electron Microscopy
4.5. Fluorescence Immunohistochemistry
4.6. Western Blotting
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rosa, I.; Romano, E.; Fioretto, B.S.; Guasti, D.; Ibba-Manneschi, L.; Matucci-Cerinic, M.; Manetti, M. Scleroderma-like Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis. Int. J. Mol. Sci. 2021, 22, 12407. https://doi.org/10.3390/ijms222212407
Rosa I, Romano E, Fioretto BS, Guasti D, Ibba-Manneschi L, Matucci-Cerinic M, Manetti M. Scleroderma-like Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis. International Journal of Molecular Sciences. 2021; 22(22):12407. https://doi.org/10.3390/ijms222212407
Chicago/Turabian StyleRosa, Irene, Eloisa Romano, Bianca Saveria Fioretto, Daniele Guasti, Lidia Ibba-Manneschi, Marco Matucci-Cerinic, and Mirko Manetti. 2021. "Scleroderma-like Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis" International Journal of Molecular Sciences 22, no. 22: 12407. https://doi.org/10.3390/ijms222212407