Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method
Abstract
:1. Introduction
2. Results
2.1. Isolation of DFAT Cells and ASCs from Adipose Tissue
2.2. Osteoblastic Differentiation of DFAT Cells and ASCs In Vitro
2.2.1. Comparison of Calcification Ability by Alizarin Red Staining
2.2.2. Comparison of Alkaline Phosphatase (ALP) Activity
2.2.3. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis
2.3. Formation of Osteoid Tissue by Transplanted DFAT Cells and ASCs
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of DFAT Cells and ASCs
4.2. Mineralization Assay by Alizarin Red Staining
4.3. ELISA for Quantification of Alkaline Phosphatase
4.4. Immunohistochemical Staining of GFP and Osteocalcin
4.5. Transplantation and Histological Evaluation
4.6. Reverse Transcription-Polymerase Chain Reaction (PCR) Analysis
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Takabatake, K.; Matsubara, M.; Yamachika, E.; Fujita, Y.; Arimura, Y.; Nakatsuji, K.; Nakano, K.; Nagatsuka, H.; Iida, S. Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method. Int. J. Mol. Sci. 2021, 22, 12392. https://doi.org/10.3390/ijms222212392
Takabatake K, Matsubara M, Yamachika E, Fujita Y, Arimura Y, Nakatsuji K, Nakano K, Nagatsuka H, Iida S. Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method. International Journal of Molecular Sciences. 2021; 22(22):12392. https://doi.org/10.3390/ijms222212392
Chicago/Turabian StyleTakabatake, Kiyofumi, Masakazu Matsubara, Eiki Yamachika, Yuki Fujita, Yuki Arimura, Kazuki Nakatsuji, Keisuke Nakano, Histoshi Nagatsuka, and Seiji Iida. 2021. "Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method" International Journal of Molecular Sciences 22, no. 22: 12392. https://doi.org/10.3390/ijms222212392