Exercise Training Alleviates Cardiac Fibrosis through Increasing Fibroblast Growth Factor 21 and Regulating TGF-β1-Smad2/3-MMP2/9 Signaling in Mice with Myocardial Infarction
Abstract
:1. Introduction
2. Results
2.1. Exercise Training Up-Regulated FGF21 Expression and Alleviated Cardiac Dysfunction and Cardiac Fibrosis in the Infarcted Heart
2.2. rhFGF21 and/or AICAR Inhibitedthe Activation of TGF-β1-Smad2/3-MMP2/9 Signaling Pathway, Promoted CFs Apoptosis and Antioxidant Capacity, Reduced Collagen Synthesis in CFs with H2O2-Treatment
2.3. Exercise Training Inhibited the Activation of the TGF-β1-Smad2/3-MMP2/9 Signaling Pathway, Enhanced Antioxidant Capacity, and Reduced Cell Apoptosis via FGF21 in the Heart of Mice with MI
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Exercise Protocols
4.3. Cardiac Fibroblast Isolation and Cell Culture
4.4. Echocardiographic Measurement
4.5. Histological Staining and Analysis
4.6. Immunofluorescence Staining
4.7. TUNEL Staining
4.8. Kit Assays
4.9. Western Blotting
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ma, Y.; Kuang, Y.; Bo, W.; Liang, Q.; Zhu, W.; Cai, M.; Tian, Z. Exercise Training Alleviates Cardiac Fibrosis through Increasing Fibroblast Growth Factor 21 and Regulating TGF-β1-Smad2/3-MMP2/9 Signaling in Mice with Myocardial Infarction. Int. J. Mol. Sci. 2021, 22, 12341. https://doi.org/10.3390/ijms222212341
Ma Y, Kuang Y, Bo W, Liang Q, Zhu W, Cai M, Tian Z. Exercise Training Alleviates Cardiac Fibrosis through Increasing Fibroblast Growth Factor 21 and Regulating TGF-β1-Smad2/3-MMP2/9 Signaling in Mice with Myocardial Infarction. International Journal of Molecular Sciences. 2021; 22(22):12341. https://doi.org/10.3390/ijms222212341
Chicago/Turabian StyleMa, Yixuan, Yixin Kuang, Wenyan Bo, Qiaoqin Liang, Wenfei Zhu, Mengxin Cai, and Zhenjun Tian. 2021. "Exercise Training Alleviates Cardiac Fibrosis through Increasing Fibroblast Growth Factor 21 and Regulating TGF-β1-Smad2/3-MMP2/9 Signaling in Mice with Myocardial Infarction" International Journal of Molecular Sciences 22, no. 22: 12341. https://doi.org/10.3390/ijms222212341