Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification of GASA Genes in Populus
2.2. Evolutionary Relationships Analysis among GASA Domains and Collinearity Analysis among GASA Genes
2.3. Gene Structure and Motif Identification, Physicochemical Properties, and Subcellular Localization of GASA Gene Family
2.4. Expression Patterns of GASA Genes in Different Tissues of Populus
2.5. Responses of GASAs to Exogenous Hormones in P. euphratica Leaves
2.6. Response of GASAs to Stresses in Populus Leaves
2.7. Identification of cis-Acting Elements in the Promoter Region of GASA Genes
2.8. Analysis of PeuGASA15 Promoter Activity
3. Discussion
4. Materials and Methods
4.1. Identification of GASA Family Genes in P. trichocarpa and P. euphratica
4.2. Physical and Chemical Properties, Subcellular Localization, Multiple Alignments and Collinearity Analysis
4.3. Plant Materials and Sample Collections
4.4. RNA Extraction, cDNA Synthesis, and qRT-PCR Analysis
4.5. Subcellular Localization of PeuGASA Proteins
4.6. Arabidopsis Culture, Construction and Transformation of Vector, and GUS Activity Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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(a) Identification in the Protein Database Reported by Ma et al. | |||||
---|---|---|---|---|---|
Protein ID | Transcript ID | Gene | Gene Description | CDS (bp) | Peptide (aa) |
XP_011001772.1 | XM_011003470.1 | LOC105108948 | gibberellin-regulated protein 9-like | 333 | 110 |
XP_011015007.1 | XM_011016705.1 | LOC105118698 | gibberellin-regulated protein 9-like | 333 | 110 |
XP_011016723.1 | XM_011018421.1 | LOC105120240 | snakin-2-like | 330 | 109 |
XP_011021371.1 | XM_011023069.1 | LOC105123460 | protein RSI-1 | 288 | 95 |
XP_011027306.1 | XM_011029004.1 | LOC105127637 | protein GAST1-like | 351 | 116 |
XP_011028622.1 /XP_011028623.1 | XM_011030320.1 /XM_011030321.1 | LOC105128593 | snakin-2-like | 342 | 113 |
XP_011029010.1 | XM_011030708.1 | LOC105128863 | gibberellin-regulated protein 11-like | 357 | 118 |
XP_011029011.1 | XM_011030709.1 | LOC105128865 | gibberellin-regulated protein 1-like | 309 | 102 |
XP_011029012.1 | XM_011030710.1 | LOC105128866 | gibberellin-regulated protein 1-like | 309 | 102 |
XP_011029030.1 | XM_011030728.1 | LOC105128880 | peamaclein-like | 267 | 88 |
XP_011035751.1 | XM_011037449.1 | LOC105133442 | gibberellin-regulated protein 4 | 321 | 106 |
XP_011038137.1 | XM_011039835.1 | LOC105135117 | gibberellin-regulated protein 2 | 276 | 91 |
XP_011044353.1 | XM_011046051.1 | LOC105139569 | snakin-2-like | 330 | 109 |
XP_011045095.1 | XM_011046793.1 | LOC105140101 | gibberellin-regulated protein 1-like | 312 | 103 |
XP_011045096.1 | XM_011046794.1 | LOC105140102 | gibberellin-regulated protein 1-like | 309 | 102 |
XP_011045097.1 | XM_011046795.1 | LOC105140103 | gibberellin-regulated protein 11-like | 297 | 98 |
XP_011045379.1 | XM_011047077.1 | LOC105140300 | protein GAST1-like | 342 | 113 |
XP_011047278.1 | XM_011048976.1 | LOC105141680 | gibberellin-regulated protein 14-like | 669 | 222 |
(b) Identification in the Protein Database Reported by Zhang et al. | |||||
Protein | mRNA | Gene | CDS (bp) | Peptide (aa) | BLAST |
GWHPAAYU001024 | GWHTAAYU001024 | GWHGAAYU001024 | 477 | 158 | (XP_011047278.1) |
GWHPAAYU001570 | GWHTAAYU001570 | GWHGAAYU001570 | 267 | 88 | No |
GWHPAAYU001972 | GWHTAAYU001972 | GWHGAAYU001972 | 342 | 113 | XP_011045379.1 |
GWHPAAYU008043 | GWHTAAYU008043 | GWHGAAYU008043 | 321 | 106 | XP_011035751.1 |
GWHPAAYU008049 | GWHTAAYU008049 | GWHGAAYU008049 | 321 | 106 | XP_011035751.1 |
GWHPAAYU008363 | GWHTAAYU008363 | GWHGAAYU008363 | 288 | 95 | XP_011021371.1 |
GWHPAAYU012087 | GWHTAAYU012087 | GWHGAAYU012087 | 342 | 113 | XP_011028622.1 /XP_011028623.1 |
GWHPAAYU013554 | GWHTAAYU013554 | GWHGAAYU013554 | 330 | 109 | XP_011016723.1 |
GWHPAAYU016490 | GWHTAAYU016490 | GWHGAAYU016490 | 309 | 102 | XP_011029012.1 |
GWHPAAYU016491 | GWHTAAYU016491 | GWHGAAYU016491 | 309 | 102 | XP_011029011.1 |
GWHPAAYU025413 | GWHTAAYU025413 | GWHGAAYU025413 | 351 | 116 | XP_011027306.1 |
GWHPAAYU029870 | GWHTAAYU029870 | GWHGAAYU029870 | 309 | 102 | XP_011045096.1 |
GWHPAAYU029871 | GWHTAAYU029871 | GWHGAAYU029871 | 312 | 103 | XP_011045095.1 |
GWHPAAYU031162 | GWHTAAYU031162 | GWHGAAYU031162 | 276 | 91 | XP_011038137.1 |
Gene 1 | Gene 2 | Duplication | E-Value | Ka | Ks | Ka/Ks | Selection Pressure |
---|---|---|---|---|---|---|---|
PtrGASA2 | PtrGASA11 | WGD | 5E-16 | 0.306574081 | 1.511177265 | 0.202871025 | Purifying selection |
PtrGASA2 | PtrGASA13 | WGD | 1E-41 | 0.024592367 | 0.245409683 | 0.100209441 | Purifying selection |
PtrGASA2 | PtrGASA16 | WGD | 3E-34 | 0.189006113 | 0.854755332 | 0.221123058 | Purifying selection |
PtrGASA11 | PtrGASA13 | WGD | 8E-14 | 0.314370832 | 1.291132145 | 0.24348463 | Purifying selection |
PtrGASA11 | PtrGASA16 | WGD | 2E-14 | 0.2812637 | 1.166421628 | 0.241133818 | Purifying selection |
PtrGASAd13 | PtrGASA16 | WGD | 6E-34 | 0.175785289 | 1.055787598 | 0.166496831 | Purifying selection |
PtrGASA7 | PtrGASA9 | WGD | 1E-33 | 0.189204442 | 0.883649795 | 0.214116998 | Purifying selection |
PtrGASA14 | PtrGASA17 | WGD | 4E-68 | 0.041106177 | 0.225565616 | 0.182236008 | Purifying selection |
PtrGASA5 | PtrGASA6 | TD | 4E-31 | 0.257142422 | 0.939998969 | 0.273556068 | Purifying selection |
PtrGASA6 | PtrGASA7 | TD | 1E-34 | 0.162561762 | 0.966817469 | 0.16814111 | Purifying selection |
PtrGASA8 | PtrGASA9 | TD | 7E-34 | 0.320382084 | 1.907862372 | 0.167927251 | Purifying selection |
PtrGASA11 | PtrGASA12 | TD | 4E-30 | 0 | 0 | NaN | - |
PeuGASA6 | PeuGASA13 | WGD | 6E-49 | 0.057332466 | 0.184523724 | 0.31070512 | Purifying selection |
PeuGASA9 | PeuGASA15 | WGD | 2E-39 | 0.097292607 | 0.362138829 | 0.268661075 | Purifying selection |
PeuGASA10 | PeuGASA19 | WGD | 2E-34 | 0.195458562 | 0.965443142 | 0.202454762 | Purifying selection |
PeuGASA7 | PeuGASA8 | TD | 2E-32 | 0.204373366 | 0.898115959 | 0.227557883 | Purifying selection |
PeuGASA8 | PeuGASA9 | TD | 6E-30 | 0.185223217 | 0.962509794 | 0.192437747 | Purifying selection |
PeuGASA14 | PeuGASA15 | TD | 1E-44 | 0.253240964 | 0.898681597 | 0.281791644 | Purifying selection |
PeuGASA15 | PeuGASA16 | TD | 3E-35 | 0.297194132 | 1.782104232 | 0.166765853 | Purifying selection |
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Han, S.; Jiao, Z.; Niu, M.-X.; Yu, X.; Huang, M.; Liu, C.; Wang, H.-L.; Zhou, Y.; Mao, W.; Wang, X.; et al. Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus. Int. J. Mol. Sci. 2021, 22, 12336. https://doi.org/10.3390/ijms222212336
Han S, Jiao Z, Niu M-X, Yu X, Huang M, Liu C, Wang H-L, Zhou Y, Mao W, Wang X, et al. Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus. International Journal of Molecular Sciences. 2021; 22(22):12336. https://doi.org/10.3390/ijms222212336
Chicago/Turabian StyleHan, Shuo, Zhiyin Jiao, Meng-Xue Niu, Xiao Yu, Mengbo Huang, Chao Liu, Hou-Ling Wang, Yangyan Zhou, Wei Mao, Xiaofei Wang, and et al. 2021. "Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus" International Journal of Molecular Sciences 22, no. 22: 12336. https://doi.org/10.3390/ijms222212336