Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF
Abstract
:1. Introduction
2. Results
2.1. Regulation of the Immediate Differentiation of Post-Selected iNKT Cells by Ets1
2.2. Transcriptomic Analyses of Ets1KO Thymic iNKT Cells
2.3. Identification of ICOS as a Target Gene of Ets1
2.4. Partial Rescue of the Differentiation of Ets1KO iNKT Cells by PLZF
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Real-Time PCR
4.3. FACS Analysis and Antibodies
4.4. iNKT Cell Enrichment
4.5. RNA Sequencing of Thymic Stage 0 NKT Cells
4.6. Plasmid, Transfection, and Luciferase Assay
4.7. Preparation of Splenic and Liver iNKT Cells
4.8. Bone Marrow Transfer for PLZF and ICOS Restoration
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chuang, Y.-T.; Chuang, W.-C.; Liu, C.-C.; Liu, C.-W.; Huang, Y.-W.; Yang, H.-Y.; Ho, I.-C.; Tai, T.-S. Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF. Int. J. Mol. Sci. 2021, 22, 12199. https://doi.org/10.3390/ijms222212199
Chuang Y-T, Chuang W-C, Liu C-C, Liu C-W, Huang Y-W, Yang H-Y, Ho I-C, Tai T-S. Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF. International Journal of Molecular Sciences. 2021; 22(22):12199. https://doi.org/10.3390/ijms222212199
Chicago/Turabian StyleChuang, Ya-Ting, Wan-Chu Chuang, Chih-Chun Liu, Chia-Wei Liu, Yu-Wen Huang, Huang-Yu Yang, I-Cheng Ho, and Tzong-Shyuan Tai. 2021. "Ets1 Promotes the Differentiation of Post-Selected iNKT Cells through Regulation of the Expression of Vα14Jα18 T Cell Receptor and PLZF" International Journal of Molecular Sciences 22, no. 22: 12199. https://doi.org/10.3390/ijms222212199