IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells
Abstract
:1. Introduction
2. Results
2.1. Characterization of In Vitro Isolated and Expanded MenSCs
2.2. Effect of MenSC Priming on IDO1 Release
2.3. Phenotypic Profile of Surface Markers on MenSCs
2.4. EV Secretion Profile of MenSCs and Phenotypic Analysis of Released Particles
2.5. Effects of IFNγ and TNFα Priming in the miRNome
2.6. miRNA Target Prediction in Inflammatory Response
2.7. Validation of miRNA Analyses by qPCR
2.8. Immunomodulatory Effect of S-MenSCs and S-MenSCs* on In Vitro Activated T Cells
3. Discussion
4. Materials and Methods
4.1. Isolation, Culture, and Characterization of Human MenSCs
4.2. Priming of MenSCs and IDO1 Quantification
4.3. Phenotypic Analysis and Comparison of MenSCs and MenSCs* Surface Markers
4.4. Collection, Concentration, and Quantification of MenSC-Derived Secretomes
4.5. Characterization of Extracellular Vesicles in MenSC-Derived Secretomes
4.6. miRNA Analysis by Next-Generation Sequencing (NGS)
4.7. Validation of Transcriptomics Results by qPCR
4.8. Immunomodulatory Assays on In Vitro Activated Peripheral Blood Lymphocytes
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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de Pedro, M.Á.; Gómez-Serrano, M.; Marinaro, F.; López, E.; Pulido, M.; Preußer, C.; Pogge von Strandmann, E.; Sánchez-Margallo, F.M.; Álvarez, V.; Casado, J.G. IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells. Int. J. Mol. Sci. 2021, 22, 12177. https://doi.org/10.3390/ijms222212177
de Pedro MÁ, Gómez-Serrano M, Marinaro F, López E, Pulido M, Preußer C, Pogge von Strandmann E, Sánchez-Margallo FM, Álvarez V, Casado JG. IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells. International Journal of Molecular Sciences. 2021; 22(22):12177. https://doi.org/10.3390/ijms222212177
Chicago/Turabian Stylede Pedro, María Ángeles, María Gómez-Serrano, Federica Marinaro, Esther López, María Pulido, Christian Preußer, Elke Pogge von Strandmann, Francisco Miguel Sánchez-Margallo, Verónica Álvarez, and Javier G. Casado. 2021. "IFN-Gamma and TNF-Alpha as a Priming Strategy to Enhance the Immunomodulatory Capacity of Secretomes from Menstrual Blood-Derived Stromal Cells" International Journal of Molecular Sciences 22, no. 22: 12177. https://doi.org/10.3390/ijms222212177