Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages
Abstract
:1. Introduction
2. Results
2.1. Identification, Quantification, and Classification of Phosphopeptides
2.2. Identification of Differentially Phosphorylated Proteins
2.3. Functional Enrichment of DEPPs
2.4. Protein Phosphorylation Patterns
2.5. Protein–Protein Interaction (PPI) Network Analysis of DEPPs
3. Discussion
4. Materials and Methods
4.1. Chickens and Parasites
4.2. Protein Extraction
4.3. Trypsin Digestion
4.4. Affinity Enrichment
4.5. LC-MS/MS Analysis
4.6. Database Search
4.7. Bioinformatics Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, X.; Liu, B.; Gong, Z.; Qu, Z.; Cai, J. Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages. Int. J. Mol. Sci. 2021, 22, 12110. https://doi.org/10.3390/ijms222212110
Ma X, Liu B, Gong Z, Qu Z, Cai J. Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages. International Journal of Molecular Sciences. 2021; 22(22):12110. https://doi.org/10.3390/ijms222212110
Chicago/Turabian StyleMa, Xueting, Baohong Liu, Zhenxing Gong, Zigang Qu, and Jianping Cai. 2021. "Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages" International Journal of Molecular Sciences 22, no. 22: 12110. https://doi.org/10.3390/ijms222212110