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Article

Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering

1
Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 75, Nowon-ro, Nowon-gu, Seoul 01812, Korea
2
Program in Biomicro System Technology, Korea University, Innovation Hall, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Korea
3
Department of Biomedical Engineering, Catholic University of Daegu, 13-13, Hayang-ro, Hayang-eup, Gyeongsan-si 38430, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Barbara Zavan, Stefano Sivolella and Alfredo Ronca
Int. J. Mol. Sci. 2021, 22(21), 11600; https://doi.org/10.3390/ijms222111600
Received: 16 September 2021 / Revised: 18 October 2021 / Accepted: 25 October 2021 / Published: 27 October 2021
(This article belongs to the Special Issue 3D Printing and Biomaterials for Biological and Medical Application)
Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks. View Full-Text
Keywords: 3D printing; gelatin biomaterial ink; suture fiber; 3D scaffold; tissue engineering 3D printing; gelatin biomaterial ink; suture fiber; 3D scaffold; tissue engineering
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MDPI and ACS Style

Choi, D.J.; Choi, K.; Park, S.J.; Kim, Y.-J.; Chung, S.; Kim, C.-H. Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering. Int. J. Mol. Sci. 2021, 22, 11600. https://doi.org/10.3390/ijms222111600

AMA Style

Choi DJ, Choi K, Park SJ, Kim Y-J, Chung S, Kim C-H. Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering. International Journal of Molecular Sciences. 2021; 22(21):11600. https://doi.org/10.3390/ijms222111600

Chicago/Turabian Style

Choi, Dong J., Kyoung Choi, Sang J. Park, Young-Jin Kim, Seok Chung, and Chun-Ho Kim. 2021. "Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering" International Journal of Molecular Sciences 22, no. 21: 11600. https://doi.org/10.3390/ijms222111600

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