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Article

Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding

1
Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá DC 111321, Colombia
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PhD Programme in Biomedical and Biological Sciences, Universidad del Rosario, Carrera 24#63C-69, Bogotá DC 111221, Colombia
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Receptor-Ligand Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá DC 111321, Colombia
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Biomathematics Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá DC 111321, Colombia
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Chemistry Department, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50#26-20, Bogotá DC 111321, Colombia
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Animal Science Faculty, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A.), Calle 222#55-37, Bogotá DC 111166, Colombia
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Department of Medical Microbiology and Immunology, University of Wisconsin—Madison, Madison, WI 53706, USA
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Morgridge Institute for Research, Madison, WI 53715, USA
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Animal Disease Research Unit, USDA-ARS, 3003 ADBF, WSU, P.O. Box 647030, Pullman, WA 99164-6630, USA
10
Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogotá DC 111321, Colombia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 714; https://doi.org/10.3390/ijms22020714
Received: 26 November 2020 / Revised: 26 December 2020 / Accepted: 31 December 2020 / Published: 13 January 2021
Apical membrane antigen 1 is a microneme protein which plays an indispensable role during Apicomplexa parasite invasion. The detailed mechanism of AMA-1 molecular interaction with its receptor on bovine erythrocytes has not been completely defined in Babesia bovis. This study was focused on identifying the minimum B. bovis AMA-1-derived regions governing specific and high-affinity binding to its target cells. Different approaches were used for detecting ama-1 locus genetic variability and natural selection signatures. The binding properties of twelve highly conserved 20-residue-long peptides were evaluated using a sensitive and specific binding assay based on radio-iodination. B. bovis AMA-1 ectodomain structure was modelled and refined using molecular modelling software. NetMHCIIpan software was used for calculating B- and T-cell epitopes. The B. bovis ama-1 gene had regions under functional constraint, having the highest negative selective pressure intensity in the Domain I encoding region. Interestingly, B. bovis AMA-1-DI (100YMQKFDIPRNHGSGIYVDLG119 and 120GYESVGSKSYRMPVGKCPVV139) and DII (302CPMHPVRDAIFGKWSGGSCV321)-derived peptides had high specificity interaction with erythrocytes and bound to a chymotrypsin and neuraminidase-treatment sensitive receptor. DI-derived peptides appear to be exposed on the protein’s surface and contain predicted B- and T-cell epitopes. These findings provide data (for the first-time) concerning B. bovis AMA-1 functional subunits which are important for establishing receptor-ligand interactions which could be used in synthetic vaccine development. View Full-Text
Keywords: Babesia bovis; AMA-1; minimum region; adhesion; bovine erythrocyte; synthetic vaccine Babesia bovis; AMA-1; minimum region; adhesion; bovine erythrocyte; synthetic vaccine
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MDPI and ACS Style

Cuy-Chaparro, L.; Bohórquez, M.D.; Arévalo-Pinzón, G.; Castañeda-Ramírez, J.J.; Suárez, C.F.; Pabón, L.; Ordóñez, D.; Gallego-López, G.M.; Suárez, C.E.; Moreno-Pérez, D.A.; Patarroyo, M.A. Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding. Int. J. Mol. Sci. 2021, 22, 714. https://doi.org/10.3390/ijms22020714

AMA Style

Cuy-Chaparro L, Bohórquez MD, Arévalo-Pinzón G, Castañeda-Ramírez JJ, Suárez CF, Pabón L, Ordóñez D, Gallego-López GM, Suárez CE, Moreno-Pérez DA, Patarroyo MA. Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding. International Journal of Molecular Sciences. 2021; 22(2):714. https://doi.org/10.3390/ijms22020714

Chicago/Turabian Style

Cuy-Chaparro, Laura, Michel D. Bohórquez, Gabriela Arévalo-Pinzón, Jeimmy J. Castañeda-Ramírez, Carlos F. Suárez, Laura Pabón, Diego Ordóñez, Gina M. Gallego-López, Carlos E. Suárez, Darwin A. Moreno-Pérez, and Manuel A. Patarroyo 2021. "Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding" International Journal of Molecular Sciences 22, no. 2: 714. https://doi.org/10.3390/ijms22020714

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