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Article

The Acquisition of Colistin Resistance Is Associated to the Amplification of a Large Chromosomal Region in Klebsiella pneumoniae kp52145

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Darwin 3, Cantoblanco, 28049 Madrid, Spain
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Author to whom correspondence should be addressed.
Current address: IMDEA Water Institute, Science and Technology Campus of the University of Alcalá, Avenida Punto Com 2, 28805 Alcalá de Henares, Spain.
Int. J. Mol. Sci. 2021, 22(2), 649; https://doi.org/10.3390/ijms22020649
Received: 18 November 2020 / Revised: 23 December 2020 / Accepted: 29 December 2020 / Published: 11 January 2021
(This article belongs to the Special Issue Antibiotic Resistance: Appearance, Evolution, and Spread)
The appearance of carbapenem-resistant Klebsiella pneumoniae has increased the use of colistin as a last-resort antibiotic for treating infections by this pathogen. A consequence of its use has been the spread of colistin-resistant strains, in several cases carrying colistin resistance genes. In addition, when susceptible strains are confronted with colistin during treatment, mutation is a major cause of the acquisition of resistance. To analyze the mechanisms of resistance that might be selected during colistin treatment, an experimental evolution assay for 30 days using as a model the clinical K. pneumoniae kp52145 isolate in the presence of increasing amounts of colistin was performed. All evolved populations presented a decreased susceptibility to colistin, without showing cross-resistance to antibiotics belonging to other structural families. We did not find any common mutation in the evolved mutants, neither in already known genes, previously known to be associated with the resistance phenotype, nor in new ones. The only common genetic change observed in the strains that evolved in the presence of colistin was the amplification of a 34 Kb sequence, homologous to a prophage (Enterobacteria phage Fels-2). Our data support that gene amplification can be a driving force in the acquisition of colistin resistance by K. pneumoniae. View Full-Text
Keywords: Klebsiella pneumoniae; colistin resistance; gene amplification Klebsiella pneumoniae; colistin resistance; gene amplification
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MDPI and ACS Style

Sánchez, M.B.; Sánchez-Gorostiaga, A.; Cuesta, T.; Martínez, J.L. The Acquisition of Colistin Resistance Is Associated to the Amplification of a Large Chromosomal Region in Klebsiella pneumoniae kp52145. Int. J. Mol. Sci. 2021, 22, 649. https://doi.org/10.3390/ijms22020649

AMA Style

Sánchez MB, Sánchez-Gorostiaga A, Cuesta T, Martínez JL. The Acquisition of Colistin Resistance Is Associated to the Amplification of a Large Chromosomal Region in Klebsiella pneumoniae kp52145. International Journal of Molecular Sciences. 2021; 22(2):649. https://doi.org/10.3390/ijms22020649

Chicago/Turabian Style

Sánchez, María B., Alicia Sánchez-Gorostiaga, Trinidad Cuesta, and José L. Martínez. 2021. "The Acquisition of Colistin Resistance Is Associated to the Amplification of a Large Chromosomal Region in Klebsiella pneumoniae kp52145" International Journal of Molecular Sciences 22, no. 2: 649. https://doi.org/10.3390/ijms22020649

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