Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of the Environmental pH on the Fluorescence Spectra of AOH
2.2. Effects of Acetonitrile and Methanol on the Emission Signal of AOH
2.3. Effects of Detergents on the Emission Signal of AOH
2.4. Effects of Cations on the Emission Signal of AOH
2.5. Investigation of AOH-Mg2+ and AOH-Ca2+ Interactions Employing Molecular Modeling Studies
2.6. Probing the Analytical Application of AOH-Mg2+ Interaction to Increase the Sensitivity of HPLC-FLD Methods
3. Materials and Methods
3.1. Reagents
3.2. Fluorescence Spectroscopic Measurements
3.3. Modeling Studies
3.4. HPLC Analyses
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Complex | K (L/mol) ± SEM Hyperquad | K (L/mol) ± SEM Benesi-Hildebrand |
---|---|---|
AOH-Mg2+ | 5.86 ± 0.62 | 4.11 ± 0.61 |
AOH-Ca2+ | 1.49 ± 0.04 | 1.75 ± 0.03 |
AOH | Interacting Ion | K (L/mol) |
---|---|---|
nonionic | Mg2+ | 5.54 |
monoanionic | Mg2+ | 5.91 |
nonionic | Ca2+ | 1.80 |
monoanionic | Ca2+ | 4.80 |
LOD | LOQ | |||
---|---|---|---|---|
Method | nM | μg/L | nM | μg/L |
C8 without Mg2+ | 50 | 12.9 | 100 | 25.8 |
C8 with Mg2+ | 10 | 2.6 | 20 | 5.2 |
C18 without Mg2+ | 100 | 25.8 | 200 | 51.6 |
C18 with Mg2+ | 20 | 5.2 | 50 | 12.9 |
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Fliszár-Nyúl, E.; Lemli, B.; Kunsági-Máté, S.; Poór, M. Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin. Int. J. Mol. Sci. 2021, 22, 8692. https://doi.org/10.3390/ijms22168692
Fliszár-Nyúl E, Lemli B, Kunsági-Máté S, Poór M. Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin. International Journal of Molecular Sciences. 2021; 22(16):8692. https://doi.org/10.3390/ijms22168692
Chicago/Turabian StyleFliszár-Nyúl, Eszter, Beáta Lemli, Sándor Kunsági-Máté, and Miklós Poór. 2021. "Effects of Microenvironmental Changes on the Fluorescence Signal of Alternariol: Magnesium Induces Strong Enhancement in the Fluorescence of the Mycotoxin" International Journal of Molecular Sciences 22, no. 16: 8692. https://doi.org/10.3390/ijms22168692