Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physicochemical Analysis/Topography of PLLA Films
2.2. X-ray Photoelectron Spectroscopy Analysis
2.3. Cellular Response
3. Materials and Methods
3.1. Materials and Preparation of Samples
3.2. Laser Modification—PLLA Surface Activation
3.3. Incubation of PLLA in SBF
3.4. X-ray Photoelectron Microscopy (XPS)
3.5. Scanning Electron Microscopy (SEM)
3.6. Profilometry
3.7. Water Contact Angle Measurement
3.8. Cell Lines and Culture
3.9. Trypan Blue Exclusion Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | Sa [nm] | Sz [μm] | Ra [nm] | Rz [μm] |
---|---|---|---|---|
PLLA | 49±3 | 0.80±0.06 | 25±3 | 0.16±0.04 |
PLLA_UV_1d | 36±7 | 0.45±0.07 | 21±3 | 0.15±0.02 |
PLLA_UV_SBF_1d | 515±55 | 5.52±0.58 | 588±62 | 3.62±0.45 |
PLLA_UV_7d | 38±4 | 0.83±0.15 | 30±3 | 0.15±0.03 |
PLLA_UV_SBF_7d | 416±29 | 9.72±1.09 | 258±55 | 1.80±0.33 |
C-C/C-H 285.0 eV | C-O-C=O 287.0 eV | O=C-O 289.1 eV | C-OH 286.4 eV | C=C 284.6 eV | O/C | Ca/C | |
---|---|---|---|---|---|---|---|
Theoretical ratio | 33.3 | 33.3 | 33.3 | - | - | 0.67 | - |
PLLA | 63.5 | 16.3 | 16.3 | 3.9 | 0 | 0.25 | - |
PLLA_UV_1d | 41.9 | 25.8 | 25.6 | 0.1 | 6.6 | 0.43 | - |
PLLA_UV_7d | 39.1 | 29.0 | 26.2 | 0.6 | 5.1 | 0.48 | |
PLLA_UV_SBF_1d | 28.9 | 31.8 | 31.0 | 4.1 | 4.2 | 0.53 | 0.005 |
PLLA_UV_SBF_7d | 30.3 | 29.3 | 31.8 | 4.9 | 3.7 | 0.50 | 0.005 |
Samples Description | Laser Treatment | Incubation in Air | Incubation in SBF |
---|---|---|---|
PLLA | − | - | - |
PLLA_UV_1d | + | 1 day (24 h) | - |
PLLA_UV_7d | + | 7 days (168 h) | - |
PLLA_UV_SBF_1d | + | - | 1 day (24 h) |
PLLA_UV_SBF_7d | + | - | 7 days (168 h) |
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Szustakiewicz, K.; Kryszak, B.; Dzienny, P.; Poźniak, B.; Tikhomirov, M.; Hoppe, V.; Szymczyk-Ziółkowska, P.; Tylus, W.; Grzymajło, M.; Gadomska-Gajadhur, A.; et al. Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification. Int. J. Mol. Sci. 2021, 22, 8436. https://doi.org/10.3390/ijms22168436
Szustakiewicz K, Kryszak B, Dzienny P, Poźniak B, Tikhomirov M, Hoppe V, Szymczyk-Ziółkowska P, Tylus W, Grzymajło M, Gadomska-Gajadhur A, et al. Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification. International Journal of Molecular Sciences. 2021; 22(16):8436. https://doi.org/10.3390/ijms22168436
Chicago/Turabian StyleSzustakiewicz, Konrad, Bartłomiej Kryszak, Paulina Dzienny, Błażej Poźniak, Marta Tikhomirov, Viktoria Hoppe, Patrycja Szymczyk-Ziółkowska, Włodzimierz Tylus, Michał Grzymajło, Agnieszka Gadomska-Gajadhur, and et al. 2021. "Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification" International Journal of Molecular Sciences 22, no. 16: 8436. https://doi.org/10.3390/ijms22168436