Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon
Abstract
:1. Introduction
2. Results
2.1. Clinicopathological Characteristics of the Cohort
2.2. Presence of High-Risk HPV Subtypes and EBV by PCR
2.3. Expression Patterns of E6 and LMP1 Oncoproteins of High-Risk HPVs and EBV
2.4. Correlation of Clinicopathological Characteristics with HPV/EBV Positivity
3. Discussion
4. Materials and Methods
4.1. Sample Collection and DNA Extraction
4.2. HPV and EBV Detection by PCR
4.3. Tissue Microarray (TMA)
4.4. Immunohistochemistry (IHC)
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Characteristic | Categories | Number (%) |
---|---|---|
Gender | Male | 37 (34.6) |
Female | 70 (65.4) | |
Age | ≤50 | 32 (29.9) |
>50 | 75 (70.1) | |
Tumor Location | Recto-sigmoid colon | 46 (49) |
Other parts of colon | 48 (51) | |
Tumor Grade φ | Grade 1 (low-grade) | 72 (76.6) |
Grade 2 (high-grade) | 22 (23.4) | |
Tumor Stage (pT) * | pT1 | 6 (6.4) |
pT2 | 5 (5.3) | |
pT3 | 79 (84) | |
pT4 | 4 (4.3) | |
Lymph Node Involvement (pN) | pN0 | 52 (55.3) |
pN1 | 20 (21.3) | |
pN2 | 22 (23.4) | |
pN3 | 0 (0) |
Samples | No. of Cases | High-Risk HPV Types | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
16 | 18 | 31 | 33 | 35 | 45 | 51 | 52 | 58 | ||
EBV (+) | 27 | 15 | 24 | 7 | 4 | 17 | 9 | 11 | 11 | 20 |
EBV (−) | 67 | 22 | 12 | 6 | 0 | 10 | 13 | 14 | 9 | 6 |
Total | 94 | 37 | 36 | 13 | 4 | 27 | 22 | 25 | 20 | 26 |
p-value | 0.07 | <0.0001 *** | 0.07 | 0.006 ** | <0.0001 *** | 0.24 | 0.09 | <0.0001 *** | <0.0001 *** |
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Nagi, K.; Gupta, I.; Jurdi, N.; Yasmeen, A.; Vranic, S.; Batist, G.; Moustafa, A.-E.A. Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon. Int. J. Mol. Sci. 2021, 22, 8118. https://doi.org/10.3390/ijms22158118
Nagi K, Gupta I, Jurdi N, Yasmeen A, Vranic S, Batist G, Moustafa A-EA. Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon. International Journal of Molecular Sciences. 2021; 22(15):8118. https://doi.org/10.3390/ijms22158118
Chicago/Turabian StyleNagi, Karim, Ishita Gupta, Nawaf Jurdi, Amber Yasmeen, Semir Vranic, Gerald Batist, and Ala-Eddin Al Moustafa. 2021. "Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon" International Journal of Molecular Sciences 22, no. 15: 8118. https://doi.org/10.3390/ijms22158118
APA StyleNagi, K., Gupta, I., Jurdi, N., Yasmeen, A., Vranic, S., Batist, G., & Moustafa, A.-E. A. (2021). Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon. International Journal of Molecular Sciences, 22(15), 8118. https://doi.org/10.3390/ijms22158118