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Article

Protein Arginine Methyltransferase 1 Is Essential for the Meiosis of Male Germ Cells

1
School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Korea
2
BioMedical Research Center, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
3
Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Alessandra Ferramosca
Int. J. Mol. Sci. 2021, 22(15), 7951; https://doi.org/10.3390/ijms22157951
Received: 6 July 2021 / Revised: 21 July 2021 / Accepted: 22 July 2021 / Published: 26 July 2021
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Protein arginine methyltransferase 1 (PRMT1) is a major enzyme responsible for the formation of methylarginine in mammalian cells; however, its function in vivo is not well understood due to its early embryonic lethality in null mice exhibiting spontaneous DNA damage, cell cycle delays, and defects in check point activation. Here, we generated germ cell-specific Prmt1 knock-out (KO) mice to evaluate the function of PRMT1 in spermatogenesis. Our findings demonstrate that PRMT1 is vital for male fertility in mice. Spermatogenesis in Prmt1 KO mice was arrested at the zygotene-like stage of the first meiotic division due to an elevated number of DNA double-strand breaks (DSBs). There was a loss of methylation in meiotic recombination 11 (MRE11), the key endonuclease in MRE11/RAD50/NBS 1 (MRN) complex, resulting in the accumulation of SPO11 protein in DSBs. The ATM-mediated negative feedback control over SPO11 was lost and, consequently, the repair pathway of DSBs was highly affected in PRMT1 deficient male germ cells. Our findings provide a novel insight into the role of PRMT1-mediated asymmetric demethylation in mouse spermatogenesis. View Full-Text
Keywords: PRMT1; ADMA; spermatogenesis; meiosis; DNA double strand break repair PRMT1; ADMA; spermatogenesis; meiosis; DNA double strand break repair
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MDPI and ACS Style

Waseem, S.; Kumar, S.; Lee, K.; Yoon, B.-H.; Kim, M.; Kim, H.; Lee, K. Protein Arginine Methyltransferase 1 Is Essential for the Meiosis of Male Germ Cells. Int. J. Mol. Sci. 2021, 22, 7951. https://doi.org/10.3390/ijms22157951

AMA Style

Waseem S, Kumar S, Lee K, Yoon B-H, Kim M, Kim H, Lee K. Protein Arginine Methyltransferase 1 Is Essential for the Meiosis of Male Germ Cells. International Journal of Molecular Sciences. 2021; 22(15):7951. https://doi.org/10.3390/ijms22157951

Chicago/Turabian Style

Waseem, Sahar, Sudeep Kumar, Kanghoon Lee, Byoung-Ha Yoon, Mirang Kim, Hail Kim, and Keesook Lee. 2021. "Protein Arginine Methyltransferase 1 Is Essential for the Meiosis of Male Germ Cells" International Journal of Molecular Sciences 22, no. 15: 7951. https://doi.org/10.3390/ijms22157951

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