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Article

Expression Silencing of Glutathione Peroxidase 4 in Mouse Erythroleukemia Cells Delays In Vitro Erythropoiesis

Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Biochemistry, Charitéplatz 1, 10117 Berlin, Germany
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Academic Editor: Dimitrios Kouretas
Int. J. Mol. Sci. 2021, 22(15), 7795; https://doi.org/10.3390/ijms22157795
Received: 18 March 2021 / Revised: 20 May 2021 / Accepted: 20 July 2021 / Published: 21 July 2021
Among the eight human glutathione peroxidase isoforms, glutathione peroxidase 4 (GPX4) is the only enzyme capable of reducing complex lipid peroxides to the corresponding alcohols. In mice, corruption of the Gpx4 gene leads to embryonic lethality and more detailed expression silencing studies have implicated the enzyme in several physiological processes (e.g., embryonal cerebrogenesis, neuronal function, male fertility). Experiments with conditional knockout mice, in which expression of the Gpx4 gene was silenced in erythroid precursors, indicated a role of Gpx4 in erythropoiesis. To test this hypothesis in a cellular in vitro model we transfected mouse erythroleukemia cells with a Gpx4 siRNA construct and followed the expression kinetics of erythropoietic gene products. Our data indicate that Gpx4 is expressed at high levels in mouse erythroleukemia cells and that expression silencing of the Gpx4 gene delays in vitro erythropoiesis. However, heterozygous expression of a catalytically inactive Gpx4 mutant (Gpx4+/Sec46Ala) did not induce a defective erythropoietic phenotype in different in vivo and ex vivo models. These data suggest that Gpx4 plays a role in erythroid differentiation of mouse erythroleukemia cells but that heterozygous expression of a catalytically inactive Gpx4 is not sufficient to compromise in vivo and ex vivo erythropoiesis. View Full-Text
Keywords: cell differentiation; lipid peroxidation; oxidative stress; eicosanoids cell differentiation; lipid peroxidation; oxidative stress; eicosanoids
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MDPI and ACS Style

Rademacher, M.; Kuhn, H.; Borchert, A. Expression Silencing of Glutathione Peroxidase 4 in Mouse Erythroleukemia Cells Delays In Vitro Erythropoiesis. Int. J. Mol. Sci. 2021, 22, 7795. https://doi.org/10.3390/ijms22157795

AMA Style

Rademacher M, Kuhn H, Borchert A. Expression Silencing of Glutathione Peroxidase 4 in Mouse Erythroleukemia Cells Delays In Vitro Erythropoiesis. International Journal of Molecular Sciences. 2021; 22(15):7795. https://doi.org/10.3390/ijms22157795

Chicago/Turabian Style

Rademacher, Marlena, Hartmut Kuhn, and Astrid Borchert. 2021. "Expression Silencing of Glutathione Peroxidase 4 in Mouse Erythroleukemia Cells Delays In Vitro Erythropoiesis" International Journal of Molecular Sciences 22, no. 15: 7795. https://doi.org/10.3390/ijms22157795

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