Dimethyl Sulfoxide-Free Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells Based on Zwitterionic Betaine and Electroporation
Abstract
:1. Introduction
2. Results
2.1. Cryoprotective Properties and Cytotoxicity of Betaine
2.2. UCMSC Cryopreservation
2.3. Evaluation of UCMSCs Functions
2.4. ROS Level of UCMSCs
2.5. Construction and Cryopreservation of GFP-Fluc-UCMSCs
2.6. The In Vivo Distribution of GFP-Fluc-UCMSCs
2.7. Chemokine Receptor Expression and Cytoskeleton Integrity of UCMSCs
3. Discussion
4. Materials and Method
4.1. Animals
4.2. Cell Preparation
4.3. DSC Test
4.4. Osmotic Regulation Test
4.5. Cytotoxicity
4.6. Influence of Electroporation on Cell Viability
4.7. Cell Cryopreservation
4.8. Cell Viability Assay
4.9. Functional Characterization
4.10. ROS Detection
4.11. BLI of GFP-Fluc-UCMSC Distribution In Vivo
4.12. Immunostaining of Cell Cytoskeleton
4.13. Chemokine Receptor Expression
4.14. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gao, L.; Zhou, Q.; Zhang, Y.; Sun, S.; Lv, L.; Ma, P.; Yang, J.; Liu, M.; Zhang, L.; Wang, X.; et al. Dimethyl Sulfoxide-Free Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells Based on Zwitterionic Betaine and Electroporation. Int. J. Mol. Sci. 2021, 22, 7445. https://doi.org/10.3390/ijms22147445
Gao L, Zhou Q, Zhang Y, Sun S, Lv L, Ma P, Yang J, Liu M, Zhang L, Wang X, et al. Dimethyl Sulfoxide-Free Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells Based on Zwitterionic Betaine and Electroporation. International Journal of Molecular Sciences. 2021; 22(14):7445. https://doi.org/10.3390/ijms22147445
Chicago/Turabian StyleGao, Lei, Qianqian Zhou, Yulong Zhang, Sujing Sun, Liping Lv, Ping Ma, Jing Yang, Min Liu, Lei Zhang, Xiaohui Wang, and et al. 2021. "Dimethyl Sulfoxide-Free Cryopreservation of Human Umbilical Cord Mesenchymal Stem Cells Based on Zwitterionic Betaine and Electroporation" International Journal of Molecular Sciences 22, no. 14: 7445. https://doi.org/10.3390/ijms22147445