Next Article in Journal
Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review
Next Article in Special Issue
3,4-Dehydro-L-proline Induces Programmed Cell Death in the Roots of Brachypodium distachyon
Previous Article in Journal
Comprehensive Molecular Dissection of Dermatophilus congolensis Genome and First Observation of tet(Z) Tetracycline Resistance
Previous Article in Special Issue
Involvement of ABA Responsive SVB Genes in the Regulation of Trichome Formation in Arabidopsis
Article

Comparative Transcriptome Analysis Reveals Genetic Mechanisms of Sugarcane Aphid Resistance in Grain Sorghum

1
United States Department of Agriculture—Agricultural Research Service, U.S. Arid Land Agricultural Research Center, Maricopa, AZ 85138, USA
2
Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68588, USA
3
Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA
4
Department of Entomology, Kansas State University, Hays, KS 67601, USA
5
Agricultural Research Center, Hays, KS 67601, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Robert Hasterok and Alexander Betekhtin
Int. J. Mol. Sci. 2021, 22(13), 7129; https://doi.org/10.3390/ijms22137129
Received: 31 May 2021 / Revised: 25 June 2021 / Accepted: 26 June 2021 / Published: 1 July 2021
(This article belongs to the Special Issue Plant Cell and Organism Development 2.0)
The sugarcane aphid, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae) (SCA), has become a major pest of grain sorghum since its appearance in the USA. Several grain sorghum parental lines are moderately resistant to the SCA. However, the molecular and genetic mechanisms underlying this resistance are poorly understood, which has constrained breeding for improved resistance. RNA-Seq was used to conduct transcriptomics analysis on a moderately resistant genotype (TAM428) and a susceptible genotype (Tx2737) to elucidate the molecular mechanisms underlying resistance. Differential expression analysis revealed differences in transcriptomic profile between the two genotypes at multiple time points after infestation by SCA. Six gene clusters had differential expression during SCA infestation. Gene ontology enrichment and cluster analysis of genes differentially expressed after SCA infestation revealed consistent upregulation of genes controlling protein and lipid binding, cellular catabolic processes, transcription initiation, and autophagy in the resistant genotype. Genes regulating responses to external stimuli and stress, cell communication, and transferase activities, were all upregulated in later stages of infestation. On the other hand, expression of genes controlling cell cycle and nuclear division were reduced after SCA infestation in the resistant genotype. These results indicate that different classes of genes, including stress response genes and transcription factors, are responsible for countering the physiological effects of SCA infestation in resistant sorghum plants. View Full-Text
Keywords: sorghum; sugarcane aphid; RNA-seq; transcriptomics; gene expression sorghum; sugarcane aphid; RNA-seq; transcriptomics; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Serba, D.D.; Meng, X.; Schnable, J.; Bashir, E.; Michaud, J.P.; Vara Prasad, P.V.; Perumal, R. Comparative Transcriptome Analysis Reveals Genetic Mechanisms of Sugarcane Aphid Resistance in Grain Sorghum. Int. J. Mol. Sci. 2021, 22, 7129. https://doi.org/10.3390/ijms22137129

AMA Style

Serba DD, Meng X, Schnable J, Bashir E, Michaud JP, Vara Prasad PV, Perumal R. Comparative Transcriptome Analysis Reveals Genetic Mechanisms of Sugarcane Aphid Resistance in Grain Sorghum. International Journal of Molecular Sciences. 2021; 22(13):7129. https://doi.org/10.3390/ijms22137129

Chicago/Turabian Style

Serba, Desalegn D., Xiaoxi Meng, James Schnable, Elfadil Bashir, J. P. Michaud, P. V. Vara Prasad, and Ramasamy Perumal. 2021. "Comparative Transcriptome Analysis Reveals Genetic Mechanisms of Sugarcane Aphid Resistance in Grain Sorghum" International Journal of Molecular Sciences 22, no. 13: 7129. https://doi.org/10.3390/ijms22137129

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop